Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,472,406	C→T	R43K (AGG→AAG)	pyrL ←	pyrBI operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,472,406	0	C	T	100.0%	25.7 / NA	11	R43K (AGG→AAG)	pyrL	pyrBI operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/8);  total (3/8)

GATGTTTCGAAAGAGAGGCGGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGC  >  NC_000913/4472208‑4472613                                                                                                                                                                                                       |                                                                                                                                                                                                                gATGTTTCGAAAGAGAGGCGGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCTTGACGCCTgg                                                                                                                                                                                                       <  1:37050/209‑1 (MQ=255)                                                   ggCAATGAATTTGTGCTTAAACCGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTCTCTCTTGACGCCTGGGCAACAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATTAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACACGGTa                                                                                                              >  1:371973/1‑248 (MQ=255)                                                     cNATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGNTATNTTTCNGANATAGCGGATTAGCCANCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGANNGNNNGNGAATgggc                                                                                                                                                                <  2:304975/196‑2 (MQ=255)                                                     cAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCATTCAGTTTCGGAGCTGTCGCCAGCACCAGATTAAGGTAATCGCGAATAAGAACGTTTGTGGAAATGATATGTTTCTGCTATCGCGGATTAGCCATCTTTTATCTCTTGACGACTGGGCCAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgggc                                                                                                                                                                >  1:304975/1‑195 (MQ=255)                                                                                       gTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCg                                                                       <  1:153419/251‑1 (MQ=255)                                                                                                     tCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCgg                                                          <  1:417554/250‑1 (MQ=255)                                                                                                                          gATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc                                    <  1:410724/251‑1 (MQ=255)                                                                                                                                           cTAAGGTCGTTTAGGGAAATGATATGTTTCGGATATAGCGGAGTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACgg                                                                                                                                                       <  1:161080/119‑1 (MQ=255)                                                                                                                                                          gAAATGATATGTTTCTGATATAGCGGATTAGCCCTCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGGCAGCGTCGTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATAc                                                  <  1:305113/205‑1 (MQ=255)                                                                                                                                                            aaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgcgc  <  1:120262/251‑1 (MQ=255)                                                                                                                                                                                   naTTAGCCATCTTTTATCTCTTGACGCCTGGGCAAAAAAAAGCCCCTCGCTTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGc                                                                                                                                  >  1:42678/2‑100 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                GATGTTTCGAAAGAGAGGCGGGTACGGGTAGAGGCTTCGAAGAAACAGCTGGCAATGACTTTGTGCTTCAACAGCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGC  >  NC_000913/4472208‑4472613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑