Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,420,399	G→A	Q140Q (CAG→CAA)	ulaA →	L‑ascorbate specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,420,399	0	G	A	100.0%	75.3 / NA	25	Q140Q (CAG→CAA)	ulaA	L‑ascorbate specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

GGCATCCTCACCAGCACCTTCAAACCGGTGGTGGCGAAAATGTCCGAAGTCTACGGCATTAACGGCGCAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAGGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGTTTCTCCATCGGTCACCAGCAGCAGTTTGCATCATGGATTGCCTATAAAGTCGCGCCGTTCCTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTC  >  NC_000913/4420157‑4420639                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ggCATCCTCACCAGCAACTTCAAACAGGTGGTGGCGAAAATGTCCGAAGTCTACGGCATTAACGGCGCAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGAGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTAAGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGtt                                                                                                                                                                                                                                          >  1:296029/1‑251 (MQ=255)         cACCAGCACCTTCAAACCGGTGGTGGCGAAAATGTCCGAAGTCTACGGCATTAACGGCGCAATTTCCGATTCCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATGACCGGCATTCGCGCAATCATGTTTACCGGCCGCATCATGTTCCAGCAAGCCGGGTTGATTGc                                                                                                                                                                                                                                    <  1:177549/249‑1 (MQ=255)                              ggtggCGAAAATGTCCGAAGTCTACGGCATTAACGGCGCAATTTCCGAGACCTACGCTTCAATGATGGCAACCATCGACCGCATCGGCGATGCCTATAGCTGTGTGGTTTACGCCGTATCGTTAGCGCTGGCGCTGAACATCTGTTTCTTTCTGTTGCGTCGCATTACCGGTATTCGCACAATCATGTGGACCGGGTACATCTTGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCt                                                                                                                                                                                                             <  1:73814/251‑1 (MQ=255)                                            ccGAAGTCTACGGCATTAACGGCGCAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGacn                                                                                                                                                                                               <  1:129276/251‑2 (MQ=255)                                                 ntCTACGGCATTAACGGCGCAATTTCCGATACCTACGATTCATTGATGGAAACCATCGAACGAATGGGCGATGCCTATAGCTGGGAGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACAGGCATACGCACAATCATGTTGACCAGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCTGCTACTCCATGTGGACCACCAt                                                                                                                                                                                          >  1:84908/2‑251 (MQ=255)                                                 gTCTACGGCATTAACGGCGCAATTTCCGATACCTTCGCTTCAATGATGGCAACCAACGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGGTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCg                                                                                                                                                                                                                <  1:302825/229‑1 (MQ=255)                                                            tAACGGCGCAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACcg                                                                                                                                                                               >  1:235453/1‑251 (MQ=255)                                                                ggCGCAATTTCCGATCCCTACGCTTAAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCgg                                                                                                                                                                                                                                             >  1:353379/1‑185 (MQ=255)                                                                  cgcAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCTTGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTc                                                                                                                                                                         >  1:450053/1‑251 (MQ=255)                                                                    cAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTg                                                                                                                                                                       <  1:288586/251‑1 (MQ=255)                                                                         tCCGATACCTACGCTGCAATGATGGCAGCCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCTCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCgg                                                                                                                                                                                                                                             <  1:437905/176‑1 (MQ=255)                                                                          ccGATACCTACGCTTCAATGATGGCAACCATCGGCCGCATGGGCGATGACCCTGGCGGGGTGGGTTTCGCCGTCTTGTTAGCGCTGGCGCTGAAAATCTGTTACGTCCTGTTGCGTCGCATGACTGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTGCGCTGTTTATCTTAGGCTAATCCATGTGGACCACCCTTATCTGTAACCCGATTCTGGTTTCg                                                                                                                                                                 >  1:406085/1‑251 (MQ=255)                                                                                                ngCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGAt                                                                                                                                                                           >  1:53244/2‑219 (MQ=255)                                                                                                   aaCCAGCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTAGTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAATCCTAGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTAcc                                                                                                                                                                                <  1:423970/211‑1 (MQ=255)                                                                                                     ccATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCg                                                                                                                                                                                                                >  1:238809/1‑177 (MQ=255)                                                                                                         ngACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGTCATcgttgcactgggtgtac                                                                                                                                  >  1:80340/2‑235 (MQ=255)                                                                                                                                             cGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGctct                                                                                                                                                             >  1:22396/1‑188 (MQ=255)                                                                                                                                                               ggcgctgAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATCACTTCCAACATGAAGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGTTTCTCCATCGGTCACcagc                                                                            >  1:37226/1‑251 (MQ=255)                                                                                                                                                                                                             cGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGTTTTCGCTCTACGGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGAGGTCGGTGGTTTCTCCATCGGTCACCAGCAGCAGTTGGCATCAGGGATTGCCTATAAAGTCGCGCCGTTCCTCg                               <  1:183505/250‑1 (MQ=255)                                                                                                                                                                                                              gCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCCCCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAgn                                                                                                                   <  1:280685/165‑2 (MQ=255)                                                                                                                                                                                                              gCACAATCATGTTGACCGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATNACTNNNNNCannnnnnnnnn                                                                                                                              >  2:280685/1‑144 (MQ=255)                                                                                                                                                                                                                             ccGGCCACATCATGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATCACt                                                                                                                                               >  1:62215/1‑122 (MQ=255)                                                                                                                                                                                                                                         tGTTCCNNCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGctct                                                                                                                                                             <  2:22396/96‑1 (MQ=255)                                                                                                                                                                                                                                         tGTTCCAGCAAGCCGGGTTGATTGCCGTTACGCTGCTTATCTTCGGCTAATCCATGTGGACCACCATTATCTGTCCCGCGATTCTGGTTTAGCTCTACTGGGGCATCACTTCCAACATGATGTCCAAGCCGACTCAGGAAGTGACGGATGGCTGTGGTTTCTCCATCGGTCACCAGCAGCAGTTTGCATCATGGATTGCCTATAAAGTCGCGCCGTTCCTCGGCAACAAAGAGGAGAGCGTTGAAGACCTc  >  1:174176/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccAGCAAGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGTTTCTCCATCGGTCACCAGCAGCAGTTTGCATCATGGAtt                                                        >  1:74587/1‑193 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GGCATCCTCACCAGCACCTTCAAACCGGTGGTGGCGAAAATGTCCGAAGTCTACGGCATTAACGGCGCAATTTCCGATACCTACGCTTCAATGATGGCAACCATCGACCGCATGGGCGATGCCTATAGCTGGGTGGGTTACGCCGTATTGTTAGCGCTGGCGCTGAACATCTGTTACGTGCTGTTGCGTCGCATTACCGGCATTCGCACAATCATGTTGACCGGCCACATCATGTTCCAGCAGGCCGGGTTGATTGCCGTTACGCTGTTTATCTTCGGCTACTCCATGTGGACCACCATTATCTGTACCGCGATTCTGGTTTCGCTCTACTGGGGCATCACTTCCAACATGATGTACAAGCCGACTCAGGAAGTGACGGATGGCTGTGGTTTCTCCATCGGTCACCAGCAGCAGTTTGCATCATGGATTGCCTATAAAGTCGCGCCGTTCCTCGGCAAAAAAGAGGAGAGCGTTGAAGACCTC  >  NC_000913/4420157‑4420639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑