Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,416,177 C→T G39D (GGT→GAT)  yjfN ← protease activator YjfN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,416,1770CT94.4% 45.9 / NA 18G39D (GGT→GAT) yjfNprotease activator YjfN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/8);  minor base G (0/1);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGT  >  NC_000913/4415951‑4416380
                                                                                                                                                                                                                                  |                                                                                                                                                                                                           
gAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCCGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACg                                                                                                                                                                                      <  1:22112/250‑1 (MQ=255)
            ccGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACCGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTgg                                                                                                                                                                         >  1:311158/1‑251 (MQ=255)
                 cAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCATGCTGCTGGTCTTCGGACATGGTAATAATGCGGTACCAGGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCGCCTGAAATATTGCTAACTGAGATCTGAGCTAGTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAAt                                                                                                                                                                    <  1:200307/251‑1 (MQ=255)
                     gcgcTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTg                                                                                                                                                                <  1:223542/251‑1 (MQ=255)
                                    ccGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCGGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTg                                                                                                                                                  <  1:396321/250‑1 (MQ=255)
                                                         ngATGACACTAATGCATACAGTATCGCCTGTACGCGCCAGATATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTATGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACt                                                                                                                             >  1:280939/2‑250 (MQ=255)
                                                                             gTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGCAATCCTGAGGTACCATGACGCACCTTGTTGATCGGCCTTCAACGCCACGATACGTTCCACGCCCTGCGGATCCGCTGAACTATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCa                                                                                                                                                              >  1:425654/1‑197 (MQ=255)
                                                                                                     cAGGCTGCTGGTCTTTGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATGGTTTCATCGTCAGCTcc                                                                                <  1:361512/251‑1 (MQ=255)
                                                                                                              ggTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGGTTCATCGTCAGCTCCATTTTTCg                                                                        <  1:355525/250‑1 (MQ=255)
                                                                                                                                     cGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATGTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGt                                                                                                     <  1:165540/198‑1 (MQ=255)
                                                                                                                                         aCCATGACGCACCTTGTTCCTCGGCCTTTAACGCCACGATACGTTCCGCGGCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTAATCTTGTTCATTCAGGcnc                                                                                                                                                                                            <  1:349387/107‑1 (MQ=255)
                                                                                                                                                 gCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCAACGTCCTGCGGATCCCCTGAAATATTGCTCACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAg                                                                                                           >  1:410272/1‑180 (MQ=255)
                                                                                                                                                     cTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGACATATTGCTAACTGAGATCTGATCTATTTCCTTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTg                                                                                                                                                  >  1:452539/1‑137 (MQ=255)
                                                                                                                                                                      aaCGCCACGATACGTTCCACGTCCTGCGGACCCACTGAAATATTGCTAACCGAGATCTGATCTATTTAATTCAGGACCGTCAAGCAGTCAGAACTGGCGAATTATGCAGACTGTGACGTAGCGCTCGCAGGATAAGTCACTAATTGAAGCGAGGGTGAGGCAAGTAATTGCTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATc                                           >  1:382593/1‑223 (MQ=255)
                                                                                                                                                                         gCCACGATACGTTCCAAGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGt                                      >  1:365610/1‑225 (MQ=255)
                                                                                                                                                                                   gTTCCACGTCCTGCGGATCCCCTGCAATATTGCTAACTGAGATCTGATATATTTCATTCAGGCCCGTCCCGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTACAGCCGGTAATTTATtgt  >  1:373349/1‑251 (MQ=255)
                                                                                                                                                                                       cANNNNNTGCNGATCCCCTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGc                                                                                                         <  2:280939/144‑1 (MQ=255)
                                                                                                                                                                                                     ccccTGAAATATTGCTAACTGAGATCTGATCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCCGACTGTGCCGTGGCGCTCGCAGGCTACGTCACTACTTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACg                              >  1:283610/1‑205 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                           
GAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGT  >  NC_000913/4415951‑4416380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑