Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,373	C→T	T103T (ACC→ACT)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,373	0	C	T	100.0%	29.8 / NA	12	T103T (ACC→ACT)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

CGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACG  >  NC_000913/4396199‑4396571                                                                                                                                                                               |                                                                                                                                                                                                       ngATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGcc                                                                                                                                >  1:276068/2‑247 (MQ=255)                      ccATCAAAGCAAACGCCCCGTGGCGCTCGATATCAAACAAACGGGCTTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGGGAAGGCGATTCGCTCTGGAACGCCTAAAGATGAACAAACTCTACGGCGGGTGTTCGATCTTACTGAAAACGTGAAAACCGAGGGGGTGAAGCGGCATAATGGCAGAAATTACACTGTCGCCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTccgc                                                                                                       <  1:449353/251‑1 (MQ=255)                       cATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTccgcc                                                                                                      >  1:34623/1‑251 (MQ=255)                            naGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATACTCTGGTGCAGGCGATTCGCTCTGGAACGCATAAAGCTGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACAATGTCGTCTTTACGATTAACGCCGATGTGCCGACACCGCCTCCTCCGCCGCCCg                                                                                                 >  1:45887/2‑251 (MQ=255)                                  aCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGgcagn                                                                                                                                                              <  1:85723/184‑2 (MQ=255)                                                                 ggCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAAGCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACTGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGc                                                            <  1:423142/251‑1 (MQ=255)                                                                                              nnnnnnnnnnaanNNNNTGANGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGcc                                                                                                                                <  2:276068/144‑1 (MQ=255)                                                                                                      ataatCTGGTGAAGGCGCTTCGCTCTGGAACGCATAAAGATGCACAAACGGTAAGGCTGATGCTCGATCTTACTGAACACGGCAAAACCAAAGCGGTGAAGAGGCAGAATGGCAGCAACTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCAGTGATTGCGAAACGCGTTGAAACGCACGCGGTCATAGCACCGAGCGTAACAGAACCGGCGCGCAATCAGTTTaa                        >  1:181790/1‑250 (MQ=255)                                                                                                              gTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGCTCTTACTGAAAACGGTAAAAACGAAGAGGTGAAGCGGCAGAATGGCAGCAATTACAATGTCGTCTTTACGATTAAAGCCGATGTGCCGACAcc                                                                                                                  >  1:393452/1‑152 (MQ=255)                                                                                                              gTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGaa                 >  1:420638/1‑249 (MQ=255)                                                                                                                      gATTCGCTCTGAAACGCCTAAATATGCACAAACGGTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGTCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTgc                                                                                                                         <  1:411977/137‑1 (MQ=255)                                                                                                                           gCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCGGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGGGGGGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGAGGTGCCGCCACCGCCTCCTCCGCCGCCCGGGGTTGCTAAACGCGTTGAAACGCCGGCGGTTTTCGCGCCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACg  <  1:385301/251‑1 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                       CGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACG  >  NC_000913/4396199‑4396571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑