Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,997	G→A	V357V (GTC→GTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,997	0	G	A	100.0%	41.6 / NA	15	V357V (GTC→GTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/9);  total (6/9)

GTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCC  >  NC_000913/4392800‑4393189                                                                                                                                                                                                      |                                                                                                                                                                                                 gTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGAAGATTTTATTCGTCTTGAATTGCAATGCATTTTTATTTTTATTCACAAGATGTGGATGAATCAGGAGTCCCGCCGTACCCCTTTTTCAATCACCCGCACCAGCCGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCCATCGCCCACGCTAtgtg                                                                                                                                             >  1:424565/1‑251 (MQ=255)             ncTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCgaga                                                                                                                                   >  1:273067/2‑249 (MQ=255)                  tGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTggg                                                                                                                             <  1:368842/250‑1 (MQ=255)                                                  tGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTGTTTCAATCGCCCGAACCAGACGCTGTGTGTTCGGCAATTGCACTTCAACTATGCACGCAGTTCGTCGCTCTATTTGCTGCTCAATCGGCCACTCTATGTTCCCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCaa                                                                                             <  1:243803/250‑1 (MQ=255)                                                           gaTCACTTGACGATTTTATTCGTCTTNAATTGCNNTGNGTTTTTATTTTTATTCACANGCNNNGNNNGNCTCAGNCGNNACGCGGTAANCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACNATGCACGCANNTCGTCTCTCTATTTGCTGCGCNATCNNCNNCNCTAngtn                                                                                                                                             >  2:98618/1‑192 (MQ=255)                                                           gaTCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTAtt                                                                                                                                                                       <  1:15547/167‑1 (MQ=255)                                                                          ttATTCTTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGATAAGTGGGTGCTCACCTTTACGACTTTCCAGCTCTGTCAAAATCGTTTCATCCCAATGTGCAtt                                                                     <  1:356209/250‑1 (MQ=255)                                                                            aTTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTTCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCATCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGcc                                                                  <  1:374491/251‑1 (MQ=255)                                                                                                   ttttatttttatTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGc                                            <  1:98618/250‑1 (MQ=255)                                                                                                                                               gTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGNNCAATCGnnc                                                                                                                                                       >  2:36220/1‑99 (MQ=255)                                                                                                                                               gTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTg                              <  1:36220/220‑1 (MQ=255)                                                                                                                                                 aaNNNNNTTTNAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTcc                                                                                                        <  2:273067/144‑1 (MQ=255)                                                                                                                                                        ttgcAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTGCGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTcc  <  1:2616/236‑1 (MQ=255)                                                                                                                                                                   cGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATacga                    >  1:103748/1‑210 (MQ=255)                                                                                                                                                                                                  cTTCAACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAAt                                                                                                                                                            >  1:119745/1‑43 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                 GTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCC  >  NC_000913/4392800‑4393189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑