Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,327,494	G→A	L120L (CTG→CTA)	rdcA →	regulator of diguanylate cyclase RdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,327,494	0	G	A	100.0%	34.1 / NA	15	L120L (CTG→CTA)	rdcA	regulator of diguanylate cyclase RdcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

GGCTTTACCCTGGAGTCAGCCTAATATCGCCGAACGTCACGCGATGCTGAATAATGAGTTGCGTAAAATTTCCCGACTGGAAATGGTGCTTGCAATTGTCGGTACCATGAAAGCAGGGAAATCAACCACCATTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTGCATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGATATTAAGCATCTGACCGATGTGCTGGAAATAGATAAAGATATGCGTGCGCTTATGCAGCGGATCGAAAATGGCGTCGCTTTCGAAAAATATTATCTGGGTGCCCAGCCTATTTTTCATTGTCTGAAAAGTTT  >  NC_000913/4327260‑4327694                                                                                                                                                                                                                                           |                                                                                                                                                                                                         gacTTTACCCTGGAGTCAGCCTAATATCGCCGAACGTCACGCGATGCTGACTAATGAGTTGCGTAACATTTCCCGACTGGTAATGGTGCTTGCAATTGTCGGTACCATGAAAGCAGGGAAATCAACCACCATTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATAGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTACATTTTTcaca                                                                                                                                                                                               >  1:429379/3‑246 (MQ=255)  gCTTTACCCTGGAGTCAGCCTAATATCGCCGAACGTCACGCGATGCTGACTAATGAGTCGCGTCAAATTTCCCGACTGGAACTGGAGCTTGCAATTGTCGGTACCCTGAAAGCAGGGAAATCAACCACCATTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCACGGGCAAAAGGAACCGGTACTACATTTTTCACATGTcgc                                                                                                                                                                                         >  1:388833/1‑251 (MQ=255)                           tCGCCGAACGTCACGCGATGCTGAATAATGAGTTGCGTAAAATTTCCCGCCTGGAAATGGAGCTTGCAGTTGTCGGTACCATGACAGCAGGGAACTCAACCACCATTACTGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTCCTACATTTTTCACATGTCGCGcc                                                                                                                                                                                      >  1:426198/1‑229 (MQ=255)                                                   ataatGAGTTGCGTAAAATTTCCCGACTGGAAATGGTGCTTGCAATTGTCGGTACCATGAAAGCAGGGAAATCAACCACCATTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCATCAATTACAACAGCGCCTGCGTGATTGc                                                                                                                                        <  1:378220/251‑1 (MQ=255)                                                         ngTTGCGTAAAATTTCCCGACTGGAAATGGTGCTTGCAATTGTAGGTAACATGCAAGCAGGGAAAACAACCAACATTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGCAACCGGTACTACATTTTTCACATGTCGCGCCAATCGCTTGTTTACTTCAACAATTACAAAAGCGCCTGCGt                                                                                                                                              >  1:42911/2‑239 (MQ=255)                                                                                 aaaTGGTGCTTGCAATTGTCAGTACCATGAAAGCAGGGAAATCAACCACCATTAATGCCATTGTGTGTACGGAGGTTCTGTCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCAGACGCCCGGGCAAAAGGAACCGGTACTACATTTTTCACATGt                                                                                                                                                                                            <  1:442324/169‑1 (MQ=255)                                                                                                                                accaTTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGCCGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGATATTAAGCATCTg                                                                                                                         >  1:170071/1‑189 (MQ=255)                                                                                                                                      aaTGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTACATTTTTCACATGTcg                                                                                                                                                                                          <  1:72873/118‑1 (MQ=255)                                                                                                                                                         nnnnnnnnnntannnnnaatngCCCAAAGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGatat                                                                                                                                   <  2:273927/144‑1 (MQ=255)                                                                                                                                                           gTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGCCGCTTATTCGCCATCAGCCCGGGCAAAAGGAACCGGTACTACCTTTTTCACATGTCGCGCCAATAGCTTGTTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGATATTAAGCATCTGACCGATGTGCTGGAAATAGATAAAGATATGCGTGCGCTTATGCAGCGGATCGAAAATGGCGTCGCTTTCGAAAAATATTATCTGGGTGc                                >  1:314425/1‑251 (MQ=255)                                                                                                                                                                               cccAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGCACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCAACACTTACAACAGCGCCTGCGAGATTGCGATATTAAGCATCTGACCGATGTGCTGGAAATAGATAAAGATATGCGTGCGCTTATGCAGCGGATCGAAAATGGCGTCGCTTTCGAAAAATATTATCTGGGAGCCCAGCCTCTTTTTCATTGTc            >  1:320932/1‑251 (MQ=255)                                                                                                                                                                                     gNNTGCGCTNNCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTGTGGAATTCAACAAtt                                                                                                                                                              <  2:37617/99‑1 (MQ=255)                                                                                                                                                                                         gcgcTGCCGACGCTTATTCGCCATACGCCCGGGCAGAAGGAACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGATATTAAGCATCTGACCGATGTGCTGGAAATAGATAAAGATATGCGTGCGCTTATGCAGCGGATCGAAAATGGCGTCGCTTTCGAACAATATTATCTGGGTGCCCAGCCTATTTTTCATTGTCTGAAAAGttt  >  1:342367/1‑251 (MQ=255)                                                                                                                                                                                                cGACGCTTATTCGCCATGCGCCCGGGCAAAAGGAACCGGTACGACATTTTTCACATGTCGCGCTAATCGAGTGGTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGATATTAATCATCGGACCGATGTGCTGGAAATAGATAAAGATATGCGTGCGCTTATGCAGCGGATc                                                                      <  1:206554/176‑1 (MQ=255)                                                                                                                                                                                                            gCCATACGCCCGGGCAAAAGGCACCGGTACTACATTTTTCACATGTCGCGCCAATCGATTATTTACTTCAACACTTACAACAGCGCCTGCGTg                                                                                                                                             >  1:435048/1‑93 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                         GGCTTTACCCTGGAGTCAGCCTAATATCGCCGAACGTCACGCGATGCTGAATAATGAGTTGCGTAAAATTTCCCGACTGGAAATGGTGCTTGCAATTGTCGGTACCATGAAAGCAGGGAAATCAACCACCATTAATGCCATTGTTGGTACGGAGGTTCTGCCTAATCGTAATCGCCCAATGACTGCGCTGCCGACGCTTATTCGCCATACGCCCGGGCAAAAGGAACCGGTACTGCATTTTTCACATGTCGCGCCAATCGATTGTTTAATTCAACAATTACAACAGCGCCTGCGTGATTGCGATATTAAGCATCTGACCGATGTGCTGGAAATAGATAAAGATATGCGTGCGCTTATGCAGCGGATCGAAAATGGCGTCGCTTTCGAAAAATATTATCTGGGTGCCCAGCCTATTTTTCATTGTCTGAAAAGTTT  >  NC_000913/4327260‑4327694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑