Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 342,413 A→G D430G (GAC→GGC)  yahJ → amidohydrolase family protein YahJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913342,4130AG100.0% 50.0 / NA 22D430G (GAC→GGC) yahJamidohydrolase family protein YahJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

GTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTG  >  NC_000913/342171‑342651
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              
gTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCCCTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGcctcct                                                                                                                                                                                                                                        <  1:2066/251‑1 (MQ=255)
                ntAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGt                                                                                                                                                                                                                        >  1:268207/2‑251 (MQ=255)
                gTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTT‑AGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGTCGGTg                                                                                                                                                                                                                       <  1:105728/251‑1 (MQ=255)
                                gCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGa                                                                                                                                                                                                                              <  1:400494/229‑1 (MQ=255)
                                                                     tCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGACCAGAACCTCTACCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGAGCGCCTCCTGTTCCGCCGAGGCGGGGGCGCGTATCTCGCCGAGCACCGCAACGTTCCATAAAGGGCCACTGGGGTggg                                                                                                                                                                   >  1:245922/1‑251 (MQ=255)
                                                                                   tGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTGGTGCTGGTGGGCGCCTCCTGGTCCGCCGAGGc                                                                                                                                                                                                                           <  1:157406/181‑1 (MQ=255)
                                                                                                   gTACGCGCAGCTCTCTATTCGTCCTANCGACCANNACNTCTCCCGCTCGCTGTTTTTNGCNNNTNNNGNTGTATNGCNNCTGCATGAANAAGGTGAGCGTGTATGGCCAAACGCGCAGGATGCCGCCAGCTTTGTGCTGGTGNGCGCCTCCTNNTCCGCCGAGGCGGTTGCGCGtngccnncnncnaacnccn                                                                                                                                                                                               >  2:97050/1‑175 (MQ=255)
                                                                                                                       gTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGATGAATGCAAGGGGAGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCGGTTCCGCCGAGGCGGCGGCGCGTATCTCGCCGAGCACCGCACAGTTCCATAAAGGGCAACTGGGGTGGGGGGGTGTGGCAGGGTGATGGGCATTTGAAACGACTGCAAAGGTGCATAGt                                                                                                                 >  1:165756/1‑251 (MQ=255)
                                                                                                                            aaCGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGGGAATAGTAag                                                                                                              >  1:157349/1‑249 (MQ=255)
                                                                                                                                   nnnnnntcnnNNNCTCNCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGc                                                                                                                                                                                                          <  2:268207/144‑1 (MQ=255)
                                                                                                                                    gAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGc                                                                                                    <  1:2772/251‑1 (MQ=255)
                                                                                                                                      aCCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCACAAGCGCCGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCCCGTATCTCGCCGAGAACCGCCACGTTCCATAAAGGGCAACTGGCGTGGGGGAGTGTGGCAGGGTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGccc                                                                                                  >  1:222460/1‑251 (MQ=255)
                                                                                                                                                                   ggCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGAGTATGGCCAAAAGAGCAGGATGACGCAAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAAGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGGGTGGGGGCGTGCGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTGGGGCAATCTGAATATGAgtg                                                                     >  1:175064/1‑251 (MQ=255)
                                                                                                                                                                             tGCCGCTGAATTAAAAAGGCTAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGgcgc                                                                                                                                                                                                                                             <  1:253181/73‑1 (MQ=255)
                                                                                                                                                                                cGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTgg                                                                                                                                                                         >  1:396082/1‑138 (MQ=255)
                                                                                                                                                                                        naaaaaGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTgg                                                                                                                                                                    >  1:270327/2‑135 (MQ=255)
                                                                                                                                                                                        gnaAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTgg                                                                                                                                                                    <  2:270327/135‑1 (MQ=255)
                                                                                                                                                                                                            gCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGGAGCCTCCTGTTCCGCCGAGGCGGt                                                                                                                                                                                                                        >  1:451598/1‑63 (MQ=255)
                                                                                                                                                                                                                      cAGGATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGTCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGTAGTGTGGCGGGTTGATGCGCATTTGAAACGCCTGCATCGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTggg                                                                                       <  1:398991/182‑1 (MQ=255)
                                                                                                                                                                                                                        ggATGNCGCCANCNNTGTGATGGTGGGCGCCTCCNGTTCCGCCGAGGCGGNGGCGNGTATCNCGncn                                                                                                                                                                                                        >  2:182302/1‑66 (MQ=255)
                                                                                                                                                                                                                        ggATGACGCCAGCTTTGTGCTGGTGGGCGCCTCCTGTGCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAggg                                                                                                                                                                                <  1:182302/91‑1 (MQ=255)
                                                                                                                                                                                                                                      ttGTGCTGGTGGGCGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAAACGCAACCTTCCCTAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCAGCAACGGTGAATAGTAAGAGATTTAAGCCCCCGGGAGAGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACCATATCTTTGAGGAATATGCCCTTATTGAAGTTGTGTAGATAAATATTTg  >  1:412557/1‑251 (MQ=255)
                                                                                                                                                                                                                                             ggtggGCGCCTCCTGTTCCGCCGAGGCTGTGTCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGtgtg                                                                                                                                                           <  1:258369/91‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              
GTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCTCCTGTTCCGCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTG  >  NC_000913/342171‑342651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑