Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,270,596	G→A	G120E (GGG→GAG)	yjbQ →	PF01894 family protein YjbQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,270,596	0	G	A	100.0%	37.6 / NA	16	G120E (GGG→GAG)	yjbQ	PF01894 family protein YjbQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/2);  total (14/2)

ATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGCCCGGAGCTGGCGGAGCTGC  >  NC_000913/4270404‑4270835                                                                                                                                                                                                 |                                                                                                                                                                                                                                                aTCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTa                                                                                                                                                                                        >  1:163258/1‑250 (MQ=255)                                 ttCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCACGACGGATCGCGTCGCATCATCGCGACACTCCAAGGGGAGTAAAAAATGCCCATTTCGGAGATGCTACAATATTg                                                                                                                                                       >  1:212596/1‑251 (MQ=255)                                    ctccGCACCGTACCCAACAACGGAACTTATGCGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGACCATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATAGCATCCACGGCGGATCGCGTCGCATCATCGCGac                                                                                                                                                                                                       >  1:356652/1‑200 (MQ=255)                                         cACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCa                                                                                                                                                >  1:159806/1‑250 (MQ=255)                                                                  gAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCACGGCGGATc                                                                                                                                                                                                                         >  1:75527/1‑152 (MQ=255)                                                                             tGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATAGCTTGTATTTCCGGTGCATAACGGCCGTCTTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCCCGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAACATGACCATTTCGGAGTTGCTACAATATTGCATGGCACAACCAGGCGCCGAACAGAGCGTGCATAATGACTgg                                                                                                            >  1:170902/1‑250 (MQ=255)                                                                                               gCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAgg                                                                                                                                        >  1:179847/1‑204 (MQ=255)                                                                                                                 cTCAATGCTGGGAACATCGCTTGTATTGCCGGAGCATAAAGGGCGTATTAAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATACACGGCGGATCGCGTCGCATCATCGCGACACTAAAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGCCTGGAAAGAGACGCAGATCAAAGTGGAAGATGTACTGTTTg                                                                       >  1:381948/1‑251 (MQ=255)                                                                                                                                                   naTAAAGGGCGTATTCAGACCGGCACCTGGCACGGCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGt                                     >  1:114366/2‑251 (MQ=255)                                                                                                                                                      aaaGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGATGGTGCATAAGGACTGGAAAGCGACGCAGATCAAAGTTGAGGATGTACTGTTTGCGATGGTGAAAGAATTAGAAAATCGCCCAGCTGttt                                   <  1:252763/250‑1 (MQ=255)                                                                                                                                                                                    ggCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCCTCGCGACACTACAAGGTGAGTAAAACATGACCATTTCGGAGTTGCTACAATAttt                                                                                                                                                       >  2:277975/1‑103 (MQ=255)                                                                                                                                                                                    ggCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAACGTGGaa                                                                                    >  1:9760/1‑171 (MQ=255)                                                                                                                                                                                    ggCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGGAAAAAATGACCATTTCGGAGTTGCTACAATAttn                                                                                                                                                       <  1:277975/104‑2 (MQ=255)                                                                                                                                                                                     gCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGc                                        >  1:427730/1‑214 (MQ=255)                                                                                                                                                                                     gCATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCAACGCGCCACTACAAGGGGAGTAAAAAATGCCCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACCGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGAGGACGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGATTCGCTGAACACCAGCCCGGAGCTGGCGGAgctg   >  1:207665/1‑251 (MQ=255)                                                                                                                                                                                      cATTTGGCTGGAGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGCTGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGCCCGGAGCTGGCGGAgctgc  >  1:402472/1‑251 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                                                                ATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCCGGTGCATAAAGGGCGTATTCAGACCGGCACCTGGCAAGGCATTTGGCTGGGGGAACATCGCATCCACGGCGGATCGCGTCGCATCATCGCGACACTACAAGGGGAGTAAAAAATGACCATTTCGGAGTTGCTACAATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGCCCGGAGCTGGCGGAGCTGC  >  NC_000913/4270404‑4270835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑