Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,119,767	C→T	A195T (GCA→ACA)	menA ←	1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,119,767	0	C	T	100.0%	18.3 / NA	9	A195T (GCA→ACA)	menA	1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/7);  total (2/7)

CCATTTCCCGCATCACATAACGGGCTTGCTTCACCAGTAATGGTGCCGCCAGCAGGAACAGCCAGCCCCACAGGCTATGCAGCGAAAAGAGATTAAACAGCGCCAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACGACGCGCGTTCACTTCACCTAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCACCCAGACCGATATAACCATAAGGACGATTGCCCACGGTGTAGGTGATAGCGGCAATGATCGACAACCCGCCAAGAATCAGGAAACCGACAAAATCGGCCAGCG  >  NC_000913/4119527‑4119991                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 ccATTTCCCGCATCACATAACGGGCTTGCTTCACCAGTAATGGTGCCGCCAGCAGGAACAGCCAGCCCCACAGGCTATGCAGCGAAAAGAGATTAAACAGCGCCAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACGACGCGCGTTCACTTCACCTAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGTCGTTGCcan                                                                                                                                                                                                                         <  1:109729/250‑2 (MQ=255)        ccGCATCACATAACGGGCTTGCTTCACCAGTAATGGTGCCGCCAGCAGGAACAGCCAGCCCCACAGGCTATGCAGCGAAAAGAGATTAAACAGCGCCAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACGACGCGCGTTCACTTCACCTAAGCGCACCACCAGCGTGTTTTTGCCATGTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGc                                                                                                                                                                                                                 <  1:230767/251‑1 (MQ=255)                                                                    ccACAGGCTATGCAGCGAAAAGAGATTAAACAGCGCCAGACACACCAGCGAGCCCAGCAGCAGGCAGGCATGATAACGACGCGCGTTCACTTCACCTAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTcc                                                                                                                                                     <  1:74946/251‑1 (MQ=255)                                                                                                                                                                  ccTAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCACCCAGACCGATATAACCATAAGGACGATTGCCCACGGTGTAGGTGATAGc                                                       <  1:409958/251‑1 (MQ=255)                                                                                                                                                                                             ncATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCACCCAGACCGATATAACCATAAGGACGATTGCCCACGGTGTAGGTGATAGCGGCAATGATCGACAACCCGCCAAGAAt                            >  1:45367/2‑251 (MQ=255)                                                                                                                                                                                                     gcgGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCACCCAGACCGATATAACCATAAGGACGATTGCCCACGGTGTAGGTGATAGCGGCAATGATCGACAACCCGCCAAGAATCAGGAAAc                    <  1:420474/251‑1 (MQ=255)                                                                                                                                                                                                              aTTGATATCTCGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACTCTCAACCAGCCAAAGAAAACCAGTACGGAAATATCGCCCACACCGATATAACCATAAGCACGATTGcc                                                                         <  1:344736/189‑1 (MQ=255)                                                                                                                                                                                                                       ccGCAGGTTATTAATATTCAGTACTGTCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCCCCCAGACCGATATAACCATAAGGACGATTGCCCACGGTGTAGGTGATAGCGGCAATGATCGACAACCCGCCAAGAATCAGGAAACCGACAAAATCGGCCAGCg  <  1:368673/250‑1 (MQ=255)                                                                                                                                                                                                                          cAGGTTATTAATATTCAGTACTGTCGTTGCCCGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCCTGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCACCCAGACCGATATAACCATAAGGACGATTGCCCACGGCGTAGGTGATAGCGGCAATGATCGACAACCCGCCAAGAATCAGGAAACCGACAAAATCGGc       >  1:374396/1‑243 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 CCATTTCCCGCATCACATAACGGGCTTGCTTCACCAGTAATGGTGCCGCCAGCAGGAACAGCCAGCCCCACAGGCTATGCAGCGAAAAGAGATTAAACAGCGCCAGACACACCAGCGAGCCCATCAGCAGGCAGGCATGATAACGACGCGCGTTCACTTCACCTAAGCGCACCACCAGCGTGTTTTTGCCATTTTCGCGGTCGCTATTGATATCACGCAGGTTATTAATATTCAGTACTGCCGTTGCCAGCAGGCCGCATGCGGTCGCCGGAAGGATCAGTGCCGGAATCAATGTATGAGCCTGTAAATACCAGCTCCCCATGACACTCAACCAGCCAAAGAAAACCAGTACGGAAATATCACCCAGACCGATATAACCATAAGGACGATTGCCCACGGTGTAGGTGATAGCGGCAATGATCGACAACCCGCCAAGAATCAGGAAACCGACAAAATCGGCCAGCG  >  NC_000913/4119527‑4119991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑