Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,060	C→T	A55T (GCC→ACC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,060	0	C	T	100.0%	51.6 / NA	21	A55T (GCC→ACC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/8);  total (13/8)

GCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACAC  >  NC_000913/4116842‑4117300                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 gctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                                                                                                                                                  >  1:127897/1‑251 (MQ=255)           taATCTTCTAAACCGTCTCCTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGG‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAGTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTc                                                                                                                                                                                                                                         <  1:218349/218‑1 (MQ=255)                         gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                                                                                                                                                                           >  1:206720/1‑250 (MQ=255)                         gTCACGGTTTTAATGCTCGCAGATTTCTGCGGCACGCCGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTc                                                                                                                                                                                                                          >  1:373645/1‑219 (MQ=255)                              gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGtt                                                                                                                                                                                                                           <  1:297703/213‑1 (MQ=255)                                           gCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATGGGCTTGCCGGTTTCTTTTTCCCAGAAAATCGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTGGCCAGCACTTCTACCAGCGAGGAGCTTTGGGGGGTCCAGATTTCCATTGGGTCGTGTTCTACCCCACCGGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                                                                                                                                        >  1:231451/1‑251 (MQ=255)                                                                gCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATGGGGTCGTGTTCTACCCAACCTGGTGTTGTGTATATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGAt                                                                                                                                                   <  1:217600/251‑1 (MQ=255)                                                                                  nttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGC‑GGTTTCAAGCTGGTTAGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGCCTTTCGCAAGCACTTATCCCAGCAGGGAGCTTTGGGAGGTCCAGATTTCAATTGGGTCGTGTTCTACCCCACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCACATGATATTGGCATCGTGATCCATTACGAACGCGCggg                                                                                                                                 >  1:56191/2‑251 (MQ=255)                                                                                                                     cAATANN‑GGNNNCCNNCTGGGTCGTAATACCGATAGCTGNAATTTGATCGGAACAGATATCGGCTTTCGNCNGNCCTTNNACCNGCGTGGAGCTNTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                                                                                                                                              >  2:332374/1‑139 (MQ=255)                                                                                                                     cAATAGTCGGTTT‑ACGTTGGTTCGTAATACCGATATCTGCAATTTGATCGGAACTGAGATCTGCTTTCGCCTGCACTTCGTCCAGCGTGTAGCTGTGGGGGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                                                                                                                                              <  1:332374/139‑1 (MQ=255)                                                                                                                                        ggTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGTCTTGCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCAGTGTGTCGTGTTCTACCCATCCTGGTTTTGGGTATATTTGCTCAAATTCGCGCTGCGacac                                                                                                                                                                          <  1:102132/157‑1 (MQ=255)                                                                                                                                        gattCGTAACACCGATAACTGCAATTNGATCGGNNCTNATCTCGGCTTTCGCCCGCCNTTNNNCNNNCNTGGCGNTTNNGGTGGTCCANATTTCCATTGTGTCGTGTTCTACCCAACCTGGTTTTGGGAAGATTTGCTCAAANTCGCGCTGCNncac                                                                                                                                                                          >  2:102132/3‑157 (MQ=255)                                                                                                                                                                 ttGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACan                                                    <  1:263546/250‑2 (MQ=255)                                                                                                                                                                    aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTAGGTGGTCCAGATTTCTATTGGGTCGTGTTCTACCCCACCTGGTTTTGGGTAGATTTGCTCAACTTCGCGCTGCGACACGCTCATGATATTGGCATCGTGATCCATTACGAc                                                                                                                                         >  1:424816/1‑162 (MQ=255)                                                                                                                                                                                          gCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTTCCCAACCTGGGTTTGGGTAGATTTGCTCAACTTCGCGCTGCGACACGCTACTGATATTGGCATCGTGCTCCATTACGACCGCGCGGGCGCTGGTGGGGCCCTGGTCGAGCGCAACGATATATTTTTTTTCCGTCATGTTTAATTGTTCCGTAGTCATATTACAGCGCAGCTTTTTGTTc                                     >  1:249389/1‑240 (MQ=255)                                                                                                                                                                                            cAGCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGTTTTTGGCATCTTGATCCCTTACGACCGCGCGGGGGCTGGTGTGGCCCTGGTCGAGCGCAACGATATTTTTTTTTTCAGCCATGTTTATTTTTCCCGTAGTctttttaaagcg                                                  >  1:22464/1‑214 (MQ=255)                                                                                                                                                                                              ncncgtCTACCAGCGTGGAGCTTTGGGTGGTCNAGATTTCCATTGGGTCGTGTTCTNNCCNNNCTNNTTTTg                                                                                                                                                                                                         <  2:331953/67‑1 (MQ=255)                                                                                                                                                                                              gCACTTCTACCAGCGTGGAGCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACACAACCTGGgtttt                                                                                                                                                                                                         >  1:331953/1‑71 (MQ=255)                                                                                                                                                                                                                 gCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                                                                                                                                                                                                <  1:13768/46‑1 (MQ=255)                                                                                                                                                                                                                 gCTTTGGGTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                                                                                                                                                                                                >  2:13768/1‑46 (MQ=255)                                                                                                                                                                                                                      gggTGGTCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGGGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAAGAGTTGTGGTTTCCTTTTCTTCCacaacaa  >  1:368846/1‑246 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 GCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGT‑GGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACAC  >  NC_000913/4116842‑4117300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑