Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	337,418	G→A	G214D (GGC→GAC)	yahF →	putative acyl‑CoA synthetase YahF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	337,418	0	G	A	100.0%	28.3 / NA	13	G214D (GGC→GAC)	yahF	putative acyl‑CoA synthetase YahF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/9);  total (4/9)

TCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGC  >  NC_000913/337207‑337654                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             tCCGATAACTTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGTTTCTGATGAGGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCTTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTGTCTCAACTGATTGACACCGGCGGGCGCGAca                                                                                                                                                                                                        <  1:137491/250‑2 (MQ=255)          gTCTCAGTTGAAGATGAAATGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTTTCGCAACTAATTGGCACCGGCGGGCGCGACCTGAGCgcga                                                                                                                                                                                               >  1:156414/1‑247 (MQ=255)                              ggCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGCCTGTGGCACGGCGATTATCAACGACGCGGCCCTCTGTTTTGATCACGCCGTGCGTCGAGGCAACATCGGTCTTCTTGGCGCATCCGCCACCGGCAGTCAGGAGTTGAGCGTCCGCCTTCATGCATTTGGCGACGGCGTTTCGCAACTGATTAGCACCGGCGGGCGCGACCTTAGCGGGAAAATCGGCGGCCCGatgatg                                                                                                                                                                           >  1:211757/1‑250 (MQ=255)                                                                 tgatgaTGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATTAATTTGGCGACGGCTTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATcggcgg                                                                                                                                                                                     <  1:65723/205‑1 (MQ=255)                                                                                        aCGGCGATTATCAACGGCGCGTCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGGTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCAGTCATGAATTTGGCGACGGCGTTTCGCAACGGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATTCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTAtctn                                                                                                                <  1:92846/251‑2 (MQ=255)                                                                                                                 tctGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCggg                                                                                                                                                            <  1:356091/182‑1 (MQ=255)                                                                                                                   tGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGAGGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGACCCGCAAACGGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGccn                                                                                      <  1:115716/250‑2 (MQ=255)                                                                                                                             ccGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCTCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTTATAATGCTCGACGCCATCGGGATGCTTTAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCtt                                                                           <  1:385774/250‑2 (MQ=255)                                                                                                                                                     gTATTGTGGGCGCATCCGGCACGGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTGGGCGACGGTGTTTCGCAACGGATTGGGCCCGGCGGGCGCGACCAGAGCGAGAAAATCGGCGGCCTGATGAGGCTCGACGCCATCGGGGTGAGGGAAACCGTCCCGCAAACTGAAATCATTGCGCTTAGCTCCAATCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAg                                                   <  1:443659/251‑1 (MQ=255)                                                                                                                                                              ncgcATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGAACTGAGAGAGAAAATCGGGGGCCTGATGATGCTCGACGCCCTCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATTTCCCAAACGCCTGCGCCTGCGGGGGCCCg                                                                                     >  1:84085/2‑208 (MQ=255)                                                                                                                                                                    ccggcaccggcaGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTTTCGAAACTGATTGGCACCGGCGGGCCCGACCTGCGGGCGAAAATCGGCGGCCTGATGATGc                                                                                                                                                                          >  1:250863/1‑117 (MQ=255)                                                                                                                                                                                       gTTGAGCGTCCGCATTCATGAATTTGGCGACGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCGACCCCATCGGGATGCTGGAAAACGACCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGtn                                                                    <  1:42539/200‑2 (MQ=255)                                                                                                                                                                                                       cATGAATTGGGCGACGGCGTTCCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGGGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCCCTTATCTCCAACCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc  <  1:24500/250‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             TCCGATAACGTCTCAGTTGAAGATGAACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCGACGCCATCGGGATGCTGGAAAACGATCCGCAAACTGAAATCATTGCGCTTATCTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGC  >  NC_000913/337207‑337654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑