Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,110,415	C→T	S162S (AGC→AGT)	cdh →	CDP‑diacylglycerol diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,110,415	0	C	T	100.0%	38.8 / NA	18	S162S (AGC→AGT)	cdh	CDP‑diacylglycerol diphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/12);  total (6/12)

ATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGCAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGCAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGG  >  NC_000913/4110174‑4110659                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     atcgtatTAACTGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTGGGTTGGCTTGGCAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTgg                                                                                                                                                                                                                                             <  1:195174/251‑1 (MQ=255)                                   cGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGCAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTgg                                                                                                                                                                                                                                             <  1:104034/217‑1 (MQ=255)                                    gATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGCCGGCGCGTGATTTTATGAACAAAACATAAGGACAGCCGGTTCCCGATCACGAGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCCAAAACATTTTCCTCTTCATACCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCAACTGCCAGGTGGTTTGCGCGGGCATGAAt                                                                                                                                                                                                           >  1:448789/1‑250 (MQ=255)                                                                                              naanaTANGNNCNNCCGNTTCCCGATCGCGCGGTTGCTTNNGCGATCAACNCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGGNCTGATGGGCNNAANCAGCTNGNNNANNNTCNGGCGACCATCAGTAGCCGCNGGNNGCCACTNCCAGGTGGTTTGCGCGGGCATGAATa                                                                                                                                                                                                          <  2:106438/192‑1 (MQ=255)                                                                                                       gCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTatga                                                                                                                                                     <  1:7762/237‑1 (MQ=255)                                                                                                                    cGATCGCGCGGTTTCTTTGGCGATCANCTCCCGNNCCNGGAGCACGCACAACCATTTNCANNNTNNNANCTCTTNTANNCGTCCTGATNTGCGCAAACAGCTGGATAACAATCTGGCGAACATCCGTAGCCGCTGGATGCCANTg                                                                                                                                                                                                                                    >  2:97434/1‑145 (MQ=255)                                                                                                                    cGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGGATTCGTCCGGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTg                                                                                                                                                                                                                                    <  1:97434/145‑1 (MQ=255)                                                                                                                                                 cccGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCGGGATAACAATCGGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAAGACCGGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTGATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTggn                                                                                              <  1:283058/250‑2 (MQ=255)                                                                                                                                                           gCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTa                                                                                                                                    >  1:183772/1‑202 (MQ=255)                                                                                                                                                                cGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGCACATCTGGGACGCTACGGGCTGGCGATGGTACGGCAGAGt                                                                              >  1:36866/1‑251 (MQ=255)                                                                                                                                                                                             tGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAAGCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGCCGCTACTGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGacac                                                  <  1:191378/250‑1 (MQ=255)                                                                                                                                                                                                aTTCGTCCTGATGTGCGCAAACAGCTNGATAACNNTCNGGCGAACATCAGTAGCCGCNGGNNNCNNNTNCCAGGNGGNNTGCGCGGGCNTGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATNTATGATGCTNNCAGAAGAAGTACCTGAGGCGCNGGANNANNTNGGANGc                                                                                                         >  2:103491/1‑192 (MQ=255)                                                                                                                                                                                                              gcgcAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACg                                 <  1:16248/250‑1 (MQ=255)                                                                                                                                                                                                                gcAAACAGCTGGATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGTCGCGTCGGGTAACGGAAAGCGAACTGGTGCAACGCAGCCCATTTTTGATGCGGGCAGAATAAGTACCTGAGGGCCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAAc                                                                        <  1:243271/209‑1 (MQ=255)                                                                                                                                                                                                                          ggATAACAATCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGc                      <  1:360352/249‑1 (MQ=255)                                                                                                                                                                                                                          ggATAACAAGCTGGCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGACCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGc                      <  1:260219/249‑1 (MQ=255)                                                                                                                                                                                                                                      ggCGAACATCAGTAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAgg           <  1:103491/248‑1 (MQ=255)                                                                                                                                                                                                                                            cATCAGTAGCCGCTGGTTGCCACTGCCAGATGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGCAATTCAgg  >  1:375638/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     ATCGTATTAACGGTACTGAAAGTCCTTTGTTGACCGATCCTTCAACGCCGAACTTCTTTTGGTTGGCTTGGCAGGCGCGTGATTTTATGAGCAAAAAATACGGCCAGCCGGTTCCCGATCGCGCGGTTTCTTTGGCGATCAACTCCCGCACCGGGCGTACGCAAAACCATTTTCATATTCATATCTCTTGTATTCGTCCTGATGTGCGCAAACAGCTGGATAACAATCTGGCGAACATCAGCAGCCGCTGGTTGCCACTGCCAGGTGGTTTGCGCGGGCATGAATACCTGGCGCGTCGGGTAACGGAAAGCGAACTGGTACAACGCAGCCCATTTATGATGCTGGCAGAAGAAGTACCTGAGGCGCGGGAACATATGGGACGCTACGGGCTGGCGATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGG  >  NC_000913/4110174‑4110659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑