Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,085,516	G→A	H103Y (CAC→TAC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,085,516	0	G	A	100.0%	20.2 / NA	10	H103Y (CAC→TAC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

ACTCGACGCGGAAGCACACAGCATCCCGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGC  >  NC_000913/4085313‑4085759                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    aCTCGACGCGGAAGCACACAGCATCCCGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTTGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCGGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTAGATGAAAGCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGt                                                                                                                                                                                                      <  1:239617/251‑1 (MQ=255)                                            ttAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCATTTTGGCAATGCGATCAAACGCCTCTTCCCAACTGATTTGATGCCATTTATACGAACCTGGCGAACGGTATTCCGGCAACTTCAGACGGCTTTCGGAGAAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGc                                                                                                                                                           >  1:440477/1‑251 (MQ=255)                                                                     gcgcAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGACGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGTCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCg                                                                                                                                  >  1:397539/1‑251 (MQ=255)                                                                                            tcttctTTCCTCGGTTTGGCGATGCGNTCAAACNNCTNTTCCCAACTGATTTGCTGCNATNNNTNNNANCATGGNGCNNGGTCTTCCGNAAACTTCAGACGGCTTTCGGAGTAGATGAAATCCACCAGGCCCGCGCCTTTGGNACAAAGTGCNNCGCGGTTGACCGGGTGATCCGNATCNNCNNCNATANggn                                                                                                                                                                     >  2:104260/1‑192 (MQ=255)                                                                                            tcttctTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGc                                                                                                                                                           <  1:104260/203‑1 (MQ=255)                                                                                                                               ctctTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                                                                                                                                             >  1:34704/1‑118 (MQ=255)                                                                                                                                tctTCCCAACTGCTTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGCTAGATGCTTTGGCGTTTTTTGCTcc                                                                                                                                         >  1:160396/1‑185 (MQ=255)                                                                                                                                                   nncATTTANNAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                                                                                                                                                                                             <  2:34704/96‑1 (MQ=255)                                                                                                                                                                                tCCGGAAACTTCAGACGGCTTTCGGAGTAGCTGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCt                       >  1:371694/1‑251 (MQ=255)                                                                                                                                                                                                      cGGAGTAGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGc  <  1:104658/250‑1 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ACTCGACGCGGAAGCACACAGCATCCCGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATATGGAAGATAGATGCTTTGGCGTTTTTTGCTCCGTCACCGAGGCTGTACATCAACAGCCCACAGCCTACGGAACAATAGGTGCAGGTATTACGGGTTTCGCGGGTGCGCAGCAGTTTATACTGCCGGGTTTCCGCGAGTGCTACGCTGGGTGCAAAACCCAGTGCCGC  >  NC_000913/4085313‑4085759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑