Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,953,043	A→G	D187G (GAT→GGT)	ilvE →	branched‑chain‑amino‑acid aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,953,043	0	A	G	100.0%	87.8 / NA	26	D187G (GAT→GGT)	ilvE	branched‑chain‑amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

TGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGC  >  NC_000913/3952801‑3953259                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         gggCAGGGGAGTAACCCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGTTATCGCGCTGGGTGTGAACg                                                                                                                                                                                                                  <  1:342333/250‑1 (MQ=255)                 ccGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAgg                                                                                                                                                                                                  >  1:241941/1‑251 (MQ=255)                                                                                gcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCGACCGTTCACctcctc                                                                                                                                   >  1:68463/1‑251 (MQ=255)                                                                                 cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCtcctcc                                                                                                                                  <  1:356389/251‑1 (MQ=255)                                                                                     aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCcgcgn                                                                                                                              <  1:47074/251‑2 (MQ=255)                                                                                         cTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGACGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCAcccc                                                                                                                                                  >  1:200266/1‑227 (MQ=255)                                                                                                  gggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACGACCTCTCTTCCCTGCTGGTTGGTATCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGt                                                                                                                                                                                                                <  1:447085/156‑1 (MQ=255)                                                                                                        gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTCCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCAcc                                                                                                                                        >  1:451698/1‑222 (MQ=255)                                                                                                                ggTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGc                                                                                                        <  1:122207/246‑1 (MQ=255)                                                                                                                      ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGAGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGCAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAAc                                                                                             >  1:330040/1‑251 (MQ=255)                                                                                                                                          cAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCa                                                                                                                                                      >  1:372525/1‑174 (MQ=255)                                                                                                                                              cacCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGaa                                                                                                                                                                       >  1:11569/1‑153 (MQ=255)                                                                                                                                                              gcAAAAGCCGGGGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGcn                                                                                <  1:39390/224‑2 (MQ=255)                                                                                                                                                                    gCCGGTGGTAACTACCTCTCTTCCCTGCTGGCGGGTAGCGAAGCGCGCCGCCACGGTTATCCGGAAGGTCTCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCATCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTAc                                                                                                                 >  1:447861/1‑185 (MQ=255)                                                                                                                                                                     ccGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTcgcgc                                              >  1:33634/1‑251 (MQ=255)                                                                                                                                                                           gtAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATcc                                        <  1:170394/251‑1 (MQ=255)                                                                                                                                                                                tACCTCTCTGCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAGCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCCCTGAAGGCTCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGGTGTTCACCCCACCTTTCACCTCCTCcgcg                                                                                                                               <  1:365485/159‑1 (MQ=255)                                                                                                                                                                                       ctTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTGATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATAGCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACGGCGCCGTGCACCGCCTCCGCGCTGCCGGGTATGACCCGTGAGGCCGTCATCAAACGGGCGAAAGAGCGTGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTg                               <  1:246105/248‑1 (MQ=255)                                                                                                                                                                                        ttCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACggg                                                                                                                                                                                                                >  1:193110/1‑69 (MQ=255)                                                                                                                                                                                                tggtggGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAgtg                   >  1:421797/1‑251 (MQ=255)                                                                                                                                                                                                    gnnTAGCGANNCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGaa                                                                                                                                                                       <  2:11569/99‑1 (MQ=255)                                                                                                                                                                                                           gAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCgg        <  1:5304/251‑1 (MQ=255)                                                                                                                                                                                                                 cgtcgccACGGTTATCAGGAAGGTATCGCGCTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCACGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTAcggc  <  1:372425/248‑1 (MQ=255)                                                                                                                                                                                                                                               cTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGNCGAANANNCGNTNGANGTGaa                                                                                                                                                                       <  2:200625/56‑1 (MQ=255)                                                                                                                                                                                                                                               cTGGGTGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGTAGTgga                                                                                                                                                                       >  1:200625/1‑54 (MQ=255)                                                                                                                                                                                                                                               cTGGGTGTGAACGGTTATAGCTCTGAAGCCGCAGACGAAAACCTGTTTGAAGTGAAAGGGGGTTTGCTGTGCGCCGGACCGTTCACCGTCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCaaa                                                                                              <  1:418049/129‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         TGGCATGGGAGTAAACCCGCCAGCGGGATACTCAACCGACGTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTATCAGGAAGGTATCGCGCTGGATGTGAACGGTTATATCTCTGAAGGCGCAGGCGAAAACCTGTTTGAAGTGAAAGATGGTGTGCTGTTCACCCCACCGTTCACCTCCTCCGCGCTGCCGGGTATTACCCGTGATGCCATCATCAAACTGGCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGC  >  NC_000913/3952801‑3953259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑