Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,896,888	C→T	P39S (CCT→TCT)	yieH →	6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,896,888	0	C	T	100.0%	48.8 / NA	18	P39S (CCT→TCT)	yieH	6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

CGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGATTTACTTATACGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTTAAACTGATTAAAAAGGAAAGGCATGTCCCGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCA  >  NC_000913/3896662‑3897082                                                                                                                                                                                                                                   |                                                                                                                                                                                                   cGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGATTTACTTATACGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTTAAACTGATTAAAAAGGAAAGGCATGTCCCGGATAGAAGCGGAATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCCGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTc                                                                                                                                                                             >  1:386572/1‑250 (MQ=255)                  gtgAGAGACTTTTTTATTGGATTTACTTATACGGCAACGATTGCGGTGCGCAAATCGGCTCTACGAAAACGTTAAACTGATTAAAAAGGAAAGGCATGTCCCGGATAGAAGCGGTATGTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCTCGCATATGTAACGATGTGTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTAc                                                                                                                                                           <  1:236672/251‑1 (MQ=255)                                 aTTGGATTTACTTATACGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTCAAACTGATTAAAAAGGAAAGGCATGTCCCGGCTCGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAATTGTAAGAAATTATCGATAtt                                                                                                                                            >  1:254749/1‑251 (MQ=255)                                             tataCGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTTAAACTGATTAAAAAGGAAAGGCATGTCCCGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCttn                                                                                                                                  <  1:280027/249‑2 (MQ=255)                                                aCGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTTAAACTGATTAAAAAGGAAAGGCATGTCCCGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAagag                                                                                                                                                                                            >  1:226386/1‑188 (MQ=255)                                                                           nctctccgcaaaCGTTAAACTGATTAAACAGGAAAGGCATGTCCCGGATAGACGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACg                                                                                                                    >  1:129560/10‑233 (MQ=255)                                                                                           naaCTGATTAAAAAGGAAAGGAATGTCCCGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAg                                                                                                                                                                         >  1:359635/2‑164 (MQ=255)                                                                                                                     cccGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAATGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGAttn                                                         <  1:49867/250‑2 (MQ=255)                                                                                                                     cccGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTc                                                         <  1:1379/250‑1 (MQ=255)                                                                                                                                           cGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACTATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAgggg                                  <  1:386147/251‑1 (MQ=255)                                                                                                                                                 cGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAg                            <  1:315936/251‑1 (MQ=255)                                                                                                                                                       tACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGCTTCAGGAATTTGATATTACACTCGATTCTGAAGAGCTCTTCAAACGTTTCAGAGGTGTAAAACTGTACGCAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGACGCTGAACACGTTTACAGTGCAGAAGTCACTCGGCTGTTAGATTCAGAACTAGAAACCCTCGAAGGGGATGGAGCGCTcc                      >  1:165594/1‑251 (MQ=255)                                                                                                                                                                     gTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAATCGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTGCCCTTGAACATGGTGTTACGTTAGc                                                                                                               <  1:302661/148‑1 (MQ=255)                                                                                                                                                                            cATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTAGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTGAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTAGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGAGATcggcagagca  >  1:192069/1‑241 (MQ=255)                                                                                                                                                                             aTTTGCTCTCGCGCATATGTAACGATGTTTCATGAATTTGGTATGACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACGGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTGCGTTAGCGAAAACAGAAGCTGAACACGTTGACCGTGCAGAAGTCGCGCGGCTGTTCGATTCAGAACTGGAAGCCAGCGAAGGGGCTGGAgcgc                         <  1:332017/226‑1 (MQ=255)                                                                                                                                                                                          cATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAAc                                                                                                         >  1:1313/1‑133 (MQ=255)                                                                                                                                                                                                           cAGGAATTTGGTATTACGCTCGATTCTGAAGAGGTATTCAAACGTTTCACAGGTGTACAACTGTACGAAATTATCGATATTGTTTCCCTTGCACATGGAGTTACGTTAGCGAAAACAGAAACTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTc                                                              >  1:387698/1‑159 (MQ=255)                                                                                                                                                                                                                           cGCTCGATTCTGAAGAGGTATTCAAACGTTTCAAAGGTGAAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTccg                                                       >  1:251357/1‑148 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                   CGACAATAGGCGCGTTTGTGAGAGACTTTTTTATTGGATTTACTTATACGGCAACGATTGCGTTGCGCAAATCGGCTCTACGAAAACGTTAAACTGATTAAAAAGGAAAGGCATGTCCCGGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGACAGTGAAGTCATTTGCTCTCGCGCATATGTAACGATGTTTCAGGAATTTGGTATTACGCTCGATCCTGAAGAGGTATTCAAACGTTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCA  >  NC_000913/3896662‑3897082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑