Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,233	C→T	P118L (CCC→CTC)	yieE →	putative phosphopantetheinyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,233	0	C	T	100.0%	56.7 / NA	21	P118L (CCC→CTC)	yieE	putative phosphopantetheinyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

GCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGG  >  NC_000913/3893990‑3894472                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                gCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCAAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATaan                                                                                                                                                                                                                                          <  1:279712/251‑2 (MQ=255)                                                                           aTCGTCACCCTGCAGAAAGGTAAACCGGTTTTCAGTGATACAAATTTGAATTCGTTTTGAATTTCGTATGAAGGACAACTGGATGGCGTGGCGTTAACAACCGAAGGAGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGATTCATAGACCACACGCGCTCGATAAc                                                                                                                                                                                                                                          >  1:401430/1‑177 (MQ=255)                                                                              ntCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATg                                                                                                                                                                                                                       >  1:357937/2‑193 (MQ=255)                                                                              gtctcccTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCAGTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCtcatcan                                                                                                                                                             <  1:270565/247‑2 (MQ=255)                                                                              gTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCAc                                                                                                                                                             >  1:180603/1‑251 (MQ=255)                                                                                             ggTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCAGGGACTATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGACCTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGCTCAGTa                                                                                                                                                                                                     >  1:388542/1‑196 (MQ=255)                                                                                                     ggTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAAc                                                                                                                                      <  1:6932/251‑1 (MQ=255)                                                                                                                                gTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTTGCGTTAACAACCGAAGGTGAAGGTGGCCTCGATATGGATCTACAGCGTGCGACGCGCGGGTGTCATAGCCCACACGCGCTCGATAACCACACCTTTTcn                                                                                                                                                                                                                              <  1:48267/136‑2 (MQ=255)                                                                                                                                                           ggTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTg                                                                                <  1:242440/251‑1 (MQ=255)                                                                                                                                                                   tggcgtTAACAACCGAAGGTGAATGTGGCCCCGATATGGAACTACAGCGTGCGACGCGCGGGATTAATAGCCCAAACGCGCTAGATAACAACCCCTTTTCAAACAAAGCATCGCTATGGATCCGTAAACaaaaag                                                                                                                                                                                            >  1:207711/1‑133 (MQ=255)                                                                                                                                                                    ggcgtTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATg                                                                                                                                                                                                             <  1:30254/117‑1 (MQ=255)                                                                                                                                                                                     gTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCACAAACGCGCTCGATAACCACACCTTTTCCAGCAATg                                                                                                                                                                                                                       >  1:417991/1‑90 (MQ=255)                                                                                                                                                                                             ggCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGt                                              <  1:22097/251‑1 (MQ=255)                                                                                                                                                                                                  cGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGaan                                         <  1:275830/251‑2 (MQ=255)                                                                                                                                                                                                       tGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCACACAACCTCTCGATAAAAACAACTGTTATAGTAATCGATCGCTATGGATAAGTAAACAAAACGCTCCTAACCAAGCTCGGGCGCAGCACATAACGCTGCGCCGCAGAGCGCTACAACTAAACGGTGATGTTTTGAATGACGATACGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGACATGTGCTCATGTAAATAATGTAGAAGCGtt                                     >  1:286994/1‑250 (MQ=255)                                                                                                                                                                                                        ggAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTAt                                   >  1:121412/1‑251 (MQ=255)                                                                                                                                                                                                                   gTGCGACGCGCGGGCTTCCTAGCCCACACGAGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTCTGGCTCAGTCTACAACACAATCCTAAAGAAGCGCGGGCGCAGCTCCTCAAGCTGCACCGAAGCCTGCTAAACCTAACCGGTGATGTTTTGAATGACGCTCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGACATGTGCTCATGTACATCATGTAGAAGCGTTATGCGACGCGGa                         >  1:394171/1‑250 (MQ=255)                                                                                                                                                                                                                           cgcgGNNTTNNNAGNNCACACGCGCTCGATAACCACACCNTTTCCAGCAATGAATCGCTATGGCTCAGTNANCNAAACNNTCCNAACGAAGCGCNGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTCACCGGTGANGNTTTGCATGACGATCCGCGCGATCTGCAg                                                                                             >  2:334207/1‑174 (MQ=255)                                                                                                                                                                                                                           cgcgGGTTTCAGTGCCCACACGCGCTCGATAACCACACCTTTGCCAGCAATGAATCGCTATGGATCAGTAAACAAAGCGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCGGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAg                                                                                             <  1:334207/174‑1 (MQ=255)                                                                                                                                                                                                                                gTTTCATAGCCCACACGCGCTCGATAACCACACCTTTGCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGTGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCTGACGCCTGAAATGTGCTCATGTAAATCAGGTAGAAGCGTTATGCGACTCGGAAGACTTGCTGgt             <  1:379682/249‑1 (MQ=255)                                                                                                                                                                                                                                         cccACACGCGCTCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTgg  >  1:227506/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                GCCGTTTTCTGGCGTCCAGAGGTTTACTCGCAGAACTGATGTTCATGCTGTATGGCATTGGCGAATTGCCGGAAATCGTCACCCTGCCGAAAGGTAAACCGGTTTTCAGTGATAAAAATTTGCCTTCGTTTTCCATTTCCTATGCCGGGAATATGGTTGGCGTGGCGTTAACAACCGAAGGTGAATGTGGCCTCGATATGGAACTACAGCGTGCGACGCGCGGGTTTCATAGCCCACACGCGCCCGATAACCACACCTTTTCCAGCAATGAATCGCTATGGATCAGTAAACAAAACGATCCTAACGAAGCGCGGGCGCAGCTCATCACGCTGCGCCGAAGCGTGCTAAAACTAACCGGTGATGTTTTGAATGACGATCCGCGCGATCTGCAGCTGCTGCCCATTGCCGGACGCCTGAAATGTGCTCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGG  >  NC_000913/3893990‑3894472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑