Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,837,362	G→A	G137D (GGC→GAC)	setC →	putative arabinose exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,837,362	0	G	A	95.5%	57.4 / ‑5.7	22	G137D (GGC→GAC)	setC	putative arabinose exporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (9/12);  total (10/12)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTAGCGCTATTATGGGAATTCTGGTCAGTCAATTTCTGGCAAGGCACTCCGATAAACAAGGCGACCGTAAATTACTGATTCTGCTATGTTGCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGG  >  NC_000913/3837122‑3837597                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            ccGGTAGCGCTATTATGGGAATTCTGGTCAGTCAATTTCTGGCAAGGCACTCCGATAAACAAGGCGACCGTAAATTACTGATTCTGCTATGTTGCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAACCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGAcn                                                                                                                                                                                                                                   <  1:42013/251‑2 (MQ=255)                          ggTCAGTCAATTTCTGGCAAGGCACTCCGATAAACAAGGCGACCGTAAATTACTGATTCTGCTATGTTGCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCGCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAAAAGACCGTGAGACGGTCATGTTCAGTACATTTTTAcn                                                                                                                                                                                                            <  1:279330/249‑2 (MQ=255)                               gtcaATTTCTGGCAAGGCACTCCGATAAACAAGGCGACCGTAAATTACTGATTCTGCTATGTTGCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTc                                                                                                                                                                                                       >  1:5350/1‑249 (MQ=255)                                                     cGATAAACAAGGCGACCGTAAATTACTGATTCTGCTATGTTGCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTgg                                                                                                                                                                                      <  1:314954/244‑1 (MQ=255)                                                                                              gCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGTCGTACGTCTGAGTAGTTTTGCTTCCTCCGCAATCCCGCAAATGTTCGCCCTCGGCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGAtc                                                                                                                                                                                                  <  1:302007/191‑1 (MQ=255)                                                                                                           gCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCTTGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTAtc                                                                                                                         >  1:436757/1‑251 (MQ=255)                                                                                                                ccTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACAttttt                                                                                                                                                                                                               <  1:10280/160‑1 (MQ=255)                                                                                                                             tGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTggg                                                                                                                                                                                     <  1:37540/173‑1 (MQ=255)                                                                                                                                                      ctcctcTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCAGCAAATGTTCGCACTAGCCCGTGAACACGCCGAAAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATATCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATATCAACGCTGCCATCGCATTTGTTGTTTGCGGACTGCTAGTCTg                                                                              >  1:324153/1‑251 (MQ=255)                                                                                                                                                                cGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                    <  1:190544/251‑1 (MQ=255)                                                                                                                                                                       aCTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTan                                                                                                                                          <  1:117269/174‑2 (MQ=255)                                                                                                                                                                                           ccACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACTGTCATGTTCAGTACATTTTTACGTGCTCAGAtc                                                                                                                                                                                                  <  1:391211/98‑1 (MQ=255)                                                                                                                                                                                             nccGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCCGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTAt                                                                                                                                                              >  1:55919/2‑132 (MQ=255)                                                                                                                                                                                             aCCGCAAACCCGCAAATGTGCGCCCGCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTGCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTg                                                                                                                                                                                       <  1:143290/107‑1 (MQ=255)                                                                                                                                                                                              ccGCAAACCCGCAAATGTTAGCCCTCGCCCGTGCACACGCCGACAGAACAGACCGCGCGCCGGTCTTGTCCAGTACATTTTTACGTGCTGCGATATCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGCTATAGTTTTAAAGTGATGTATCTCACCGCCGCACTCGCATTTGTTGTTTGCGGACTGATAGTATGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGtt                                      >  1:403502/1‑251 (MQ=255)                                                                                                                                                                                              ccGCAAACCCGCAAATGTCCGCCCTCGCCCGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTGACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGTGATTTAGTTTTAAAGTGATGTATCTCACCTCTGCTATCGCATTTGTTGTTTGCTGACTGATAGTCTGGTTGTTTTTGCCATCAAt                                                            <  1:393087/229‑1 (MQ=255)                                                                                                                                                                                                           aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGACAGTGAGACGGACATGATCAGTACATTTTTACGTGCTAAGATCTCGCTTGCCTGGGTTATCGGGCCAACGCTCGATTATGAACTGGCAATGGGATTTAGTTTTAAAGAGATGTATCTCACCGCTGCCTTCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                           >  1:212975/1‑249 (MQ=255)                                                                                                                                                                                                                         cccGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCCCCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCATAGAAATTTTACCCTcc           >  1:61635/1‑251 (MQ=255)                                                                                                                                                                                                                         cccGTGAACACGCCGACAGAACAGACCGTGAGACGGTCATGATCAGTACCTTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGACACCGCTCGCTTATGAACTGGCACTGGGATTTAGATTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGCCTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCCACCCGTAGAAATTTTACCCTcc           >  1:302027/1‑251 (MQ=255)                                                                                                                                                                                                                                ccacGCCGACAGAACAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAACTTTTACCCTccacccat     >  1:121698/2‑249 (MQ=255)                                                                                                                                                                                                                                   cGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTCTTACGTGCTCAGATCTAGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCACTGGGCTTTAGTTCTAAAGCGCTGTCTCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTAATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAgg  >  1:70907/1‑250 (MQ=255)                                                                                                                                                                                                                                            aaCAGACCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATTGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGc                                                                                            <  1:191799/151‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            CCGGTAGCGCTATTATGGGAATTCTGGTCAGTCAATTTCTGGCAAGGCACTCCGATAAACAAGGCGACCGTAAATTACTGATTCTGCTATGTTGCTTATTTGGAGTGCTGGCCTGCACGCTTTTTGCGTGGAATCGCAACTACTTCATTCTCCTCTCAACGGGCGTACTTCTGAGTAGTTTTGCTTCCACCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGG  >  NC_000913/3837122‑3837597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑