Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,783,983	G→A	S49F (TCC→TTC)	secB ←	protein export chaperone SecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,783,983	0	G	A	100.0%	87.3 / NA	29	S49F (TCC→TTC)	secB	protein export chaperone SecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/18);  total (11/18)

CATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATCC  >  NC_000913/3783778‑3784209                                                                                                                                                                                                              |                                                                                                                                                                                                                                   cATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTg                                                                                                                                                                                               <  1:245683/243‑1 (MQ=255)              cACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTg                                                                                                                                                                          <  1:25947/251‑1 (MQ=255)                  cACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTgcgg                                                                                                                                                                       <  1:334177/250‑1 (MQ=255)                             gggAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTGCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGTAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGTCGCGTTCGgcgc                                                                                                                                                           <  1:107253/251‑1 (MQ=255)                                                   aTGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGTTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACGTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCAGGTTGCCAATCTTTCTGGAAAACGTgc                                                                                                                                                                         <  1:306852/215‑1 (MQ=255)                                                                     ccATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCCTCGCCCAAAGAGGCCGTTCCGGTAACACGCAGTACCCCTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATc                                                                                                                                                                                                                 <  1:397589/157‑1 (MQ=255)                                                                      cATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGAAACGCGGTTTCTTCGCCCAACGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTgg                                                                                                                   >  1:239037/1‑250 (MQ=255)                                                                               gCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGa                                                                                                                        <  1:17342/236‑1 (MQ=255)                                                                                ctttCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACGTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCACTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGGTGCCAATCTTTCTGGAAAACGTgcgg                                                                                                                                                                       <  1:321424/186‑1 (MQ=255)                                                                                 cTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTc                                                                                                        >  1:402281/1‑250 (MQ=255)                                                                                     gATACCCGCGATGGAGACAATACCGCCCTGCTGCACTTCACACAGGAACGCGGTTTCCTAGCCTAAAGAGGAAGTTACGCTAACACGCAGTACCACTTCGTATACCTCTTCTGCCACTTGGAAAGATGCCGTATCCAGATCAAGTTTATCTTCTGGTTGCCAATATTTCTGGAAAACGTGAGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATACATAAGTTGGATCTGGAAAGACATTTCAgtgt                                                                                                   >  1:437115/1‑251 (MQ=255)                                                                                         cccGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTAGCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGcgcg                                                                                                                                                                   <  1:8087/183‑1 (MQ=255)                                                                                                       aaTACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGCGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAg                                                                                                                                                                                                              >  1:151930/1‑126 (MQ=255)                                                                                                              cccTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCCCTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGat                                                                                                                                               >  1:204057/1‑182 (MQ=255)                                                                                                                ntgctgAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGAc                                                                                     >  1:274537/2‑238 (MQ=255)                                                                                                                  gctgAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAg                                                                                                                                                                                                                                 <  1:8036/96‑1 (MQ=255)                                                                                                                  gctgAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGNNGGAAAg                                                                                                                                                                                                                                 >  2:8036/1‑96 (MQ=255)                                                                                                                           cacacaGGAACTCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCGTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAgtgt                                                              <  1:170463/250‑1 (MQ=255)                                                                                                                            acacaGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGtgttg                                                            >  1:324343/1‑251 (MQ=255)                                                                                                                             cacaGGAACGCGGTTTCTGCGCCGAAAGAGGCCGTTACGGTAACACGCAGTACCGCTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTACTTTAAATCGCGCGGCGCGTTCGGCGCTTCGAAAGAGAGAGCCGTGTTATAAATACGTAAGATCTGGAAAGTGATTTCAGTGTTTTTTTGTTCTGACATGTGTAGAAAACCCTTAAGtgttgt                                                           <  1:74500/251‑1 (MQ=255)                                                                                                                                     cgcgGTTTCTTCGCCCAAAGAGGCCGTTAAGGTAACACGCAGTACCACTTCGTATCCGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCtt                                                                                                                                                                                       >  1:135494/1‑119 (MQ=255)                                                                                                                                       cgGTTTCTTCGCCCAAAGATGCCGGTACGGTAACACTCAGTGCCACTTCGTATTCGTCATCTGCCAGTTGGAAAGATTCCTTATCCAGATCAAGTTTAACTTCTGGTTTCCAATCTTTCTGTAAAACTTGCGTCGCGTTCGGCTCTTCGAAAGAGTTATTCTTGGTATAAATACGTTTGAACTCGAAAGGCTTTTCATTGTTGTTTTGTTCGTTCATGTATAGAAAACCCTTAAGTGGTGTCCTTAAATAc                                                 <  1:176143/251‑1 (MQ=255)                                                                                                                                            tcttcGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAATCCCTTAAGTGTTGTCCTTAAATACTgcn                                             <  1:108371/250‑2 (MQ=255)                                                                                                                                                                          ngCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGAc                                                                                     >  1:275944/2‑180 (MQ=255)                                                                                                                                                                          cGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAg                                                                 >  1:327791/1‑200 (MQ=255)                                                                                                                                                                          cGCAGGACCACTTCGTATACGTNATCTGCCAGTTGGAAAGATGCCGTATCCAGANCNAGTGTAACGTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCNGCGCTTCGAANGAGNNATCCNTNGNATAAATACGTTGGATCTGGAAAGTCATTTNAGTGTTGTTTTGTTCTGACATGTNNAGNNNACNNTTAAg                                                                 <  2:327791/200‑1 (MQ=255)                                                                                                                                                                                 ccACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGGGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAggg       <  1:237217/251‑1 (MQ=255)                                                                                                                                                                                      tCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAggg       <  1:256159/246‑1 (MQ=255)                                                                                                                                                                                       cGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATcn  <  1:275739/250‑2 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                   CATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCCTTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATCC  >  NC_000913/3783778‑3784209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑