Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 306,474 C→T G115D (GGC→GAC)  ecpD ← fimbrial adhesin EcpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913306,4740CT96.4% 67.1 / ‑3.4 28G115D (GGC→GAC) ecpDfimbrial adhesin EcpD
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (15/12);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01

GCCGCTGCTGGCGTCATAGTTCACAGAGGTCTGGCAGGCATTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGCCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGG  >  NC_000913/306236‑306718
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
gccgcTGCTGGCGTCATAGTTCACAGAGGTCTGGCAGGCATTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAgg                                                                                                                                                                                                                                            >  1:32839/1‑249 (MQ=255)
                     cacaGAGGTCTGGCAGGCATTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACGGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGGCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGGTTGCGGCAGGAGAAGAGTTTTGGTCATATTACGCCCAGCGTACCAGTTGATGCAGGGCAAGCCAGTTAAAGGAGATGAAACTGGTGAATTTTcn                                                                                                                                                                                                                      <  1:89058/250‑2 (MQ=255)
                       caGAGGTCTGGCAGGCATTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGc                                                                                                                                                                                                                   <  1:13972/251‑1 (MQ=255)
                           ggTCTGGCAGGCATTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCcac                                                                                                                                                                                                                >  1:353927/1‑250 (MQ=255)
                                   aggcaTTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGGCACCGTCGCGCCATAAAATCCACGGGGGGCGTTGGTTTGCTGTAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCTCAATTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAAc                                                                                                                                                                                                       <  1:414173/251‑1 (MQ=255)
                                                       aaGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATCCCGTGCATCCACTTCGAGCCGCCGCTGGTCACCGTCGCGCCATACAATCCACTGGCGTCGCTGGTTTGCGGCAGGATAAGACTCGTGGGCATATTACACCCAGCGTACCAGTTGATGCAGAGCAAGTCCGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGcc                                                                                                                                                                                   >  1:446294/1‑251 (MQ=255)
                                                                        cATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCCCTGACGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTAt                                                                                                                                                                  >  1:399297/1‑251 (MQ=255)
                                                                             gttgCATATACTGGTAAAACGCGTCTGACAACATGCCGCGCATCCACTTCGCGCAGCCGATGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGTTGTTGTAACCGTTATCAATg                                                                                                                                                             >  1:331343/1‑251 (MQ=255)
                                                                                                     cTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAACATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTcc                                                                                                                                                                                                                      >  1:71854/1‑170 (MQ=255)
                                                                                                       gACAACATGCCGTGTATCCTCTGCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGGCGGTGGTTGGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGATATGAAACTGGTGAATTTTCCAGCCACATTTCAAACTTCCAGTTGTTATAAAGTCCGGTGTTGTACCCGGTATCAATGTAGCCGAGGCTTTGCTGATAGATg                                                                                                                                     <  1:230112/249‑1 (MQ=255)
                                                                                                         aaacaTGCCCTGAAACCGCTTCGCGCCGCCGCTGGTCCCCGTCGCGCCCTAAAATCCACTGGGGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACCCCCAGCGTGCCAGTTGAAGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGGTATCAATGTCGCCGAGGCTTTGCTGATAGATGGTTc                                                                                                                                 <  1:2082/250‑1 (MQ=255)
                                                                                                                 cGTGCATCCAATTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGAtcg                                                                                                                                        >  1:119536/1‑234 (MQ=255)
                                                                                                                          aCTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAACATCCACTGCCGGCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCAGCGTATCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGCTGAAACTGGTGCATTTTCCAGCCACATATCAAACTTCCAGTTGGTTTAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGGGTAtttt                                                                                                                >  1:219793/1‑250 (MQ=255)
                                                                                                                                       ncTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATTTTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTCAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGcc                                                                                                                                                        >  1:53855/2‑198 (MQ=255)
                                                                                                                                          ggTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCCGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGga                                                                                                                                                                                                                                           >  1:163921/1‑112 (MQ=255)
                                                                                                                                           gTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATCGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGtt                                                                                               >  1:94270/1‑251 (MQ=255)
                                                                                                                                             cACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTAATATTACACCCAGCGTACCAGTTGATGCAGCGCACGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCCGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGt                                                                                                        >  1:304786/1‑240 (MQ=255)
                                                                                                                                                       ccATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAg                                                                                                                                                          <  1:194081/180‑1 (MQ=255)
                                                                                                                                                            aaaTCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGcgcg                                                                              <  1:21076/251‑1 (MQ=255)
                                                                                                                                                                     ggCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAg                                                                       <  1:7587/249‑1 (MQ=255)
                                                                                                                                                                                                aCTGGTGGTCAAATTACACCCACCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATAAAACGGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTGGTACCCGTTATCAAGGGATCCAAGGCTTTGCTGATAGAGGGTCCCTGAACCAGTGTATTGAAACCCGGTCGAGCGATGGGCGCCGGCCAGGCTCGTACCCATCCCCCCGCCAGGAGTGACGAAAAAGTTgt                                          <  1:146177/251‑1 (MQ=255)
                                                                                                                                                                                                           tatTACACCCAGCGGACCAGTGGATGCAACGCAAGTCAGTGAAAGGAGATGAAACTGGTGAAGTCTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAAcc                                                                                                                                                                       <  1:227903/115‑1 (MQ=255)
                                                                                                                                                                                                                   ccAGCGTACCAGTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATc                                                                                                                                                                                                           >  1:74916/1‑71 (MQ=255)
                                                                                                                                                                                                                          aNNAGTTGANNCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTAAAAAGGCCGGTGTTGTAAc                                                                                                                                                                        <  2:32839/99‑1 (MQ=255)
                                                                                                                                                                                                                              gTTGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACt            >  1:136255/1‑251 (MQ=255)
                                                                                                                                                                                                                               ttGATGCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGGGTTGTAACCGGTATCAATGTAGCCGAGGCTTGGCTGATAGAGCGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGgcg                                                                                <  1:226037/182‑1 (MQ=255)
                                                                                                                                                                                                                                   ggCAGCGCAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGTCCGGTGTTGTAACCGTTATCAATGTAGCCGAGTCTTTGCTGATAGATTGTTCCTGAACCAGTGTATTTTAAAcc                                                                                                           <  1:403727/150‑1 (MQ=255)
                                                                                                                                                                                                                                        ngcAAGTCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCgg  >  1:47140/2‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    
GCCGCTGCTGGCGTCATAGTTCACAGAGGTCTGGCAGGCATTGATGGTCATTGTAAAGCTGCTGCCGACGGGCATTTGTTGCATATACTGGTAAAACGCGTCTGACAACATGCCGTGCATCCACTTCGCGCCGCCGCTGGTCACCGTCGCGCCATAAAATCCACTGGCGTCGGTGGTTTGCGGCAGGATAAGACTGGTGGTCATATTACACCCAGCGTACCAGTTGATGCAGCGCAAGCCAGTTAAAGGAGATGAAACTGGTGAATTTTCCAGCCACATATCAAACTTCCAGTTGGTATAAAGGCCGGTGTTGTAACCGTTATCAATGTAGCCGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGG  >  NC_000913/306236‑306718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: