Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	57.1 / NA	22	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/11);  total (11/11)

GCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAA  >  NC_000913/3707712‑3708189                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   gCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGCTGCCGTACTAATTAACATCAGTGTAACCGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCACCCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaaaa                                                                                                                                                                                                                                      >  1:368438/1‑250 (MQ=255)                                    cAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCACGGCCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACCGAATGTGAAAACGTCAATACAGCCAACAGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtgtg                                                                                                                                                                                                  >  1:67318/1‑251 (MQ=255)                                                                           cGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACGTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAg                                                                                                                                                           <  1:243840/251‑1 (MQ=255)                                                                                  gTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgn                                                                                                                                                                                                                  <  1:48869/189‑2 (MQ=255)                                                                                                                           gtgtAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCGACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTGCAAACAGCGATTTGCAGCAGAGAAATGTGTGATACTCCAACATGACCAGAACAAGCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTa                                                                                                                                    <  1:231315/226‑1 (MQ=255)                                                                                                                                     gCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAAc                                                                                                                                                                             >  1:177049/1‑175 (MQ=255)                                                                                                                                           cctccATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTTCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATg                                                                                                                                  <  1:288807/212‑1 (MQ=255)                                                                                                                                                                 aCCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGAtt                                                                                                                                            >  1:396649/1‑180 (MQ=255)                                                                                                                                                                            gTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                                                                                                                      <  1:398879/143‑1 (MQ=255)                                                                                                                                                                              naaCAGAATGTGAAAACGTCCATACAGCCCACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCt                                                                                                                                                                                                >  1:109724/2‑115 (MQ=255)                                                                                                                                                                               aaCAGAATGTGAAAACGTCAATACAGCCAACCGGGCTTTACACCAACGGTGCGCATCCAAAAACAAAGATTAACAAAACTTCAAACAGCTATTTgcagc                                                                                                                                                                                                               >  1:388359/1‑99 (MQ=255)                                                                                                                                                                               aaCAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCgg                                                       <  1:74823/251‑1 (MQ=255)                                                                                                                                                                                             aCGTCAATACAGCCAACCGGGATTTACCCCAACGGTGAGAATCCACAAACCCAGATTCAAAAAACTTCAAAAAGCTATTTGCAGCAGCGAAATTTGTGCTACTCCAACATGCCCAGACCAATccgc                                                                                                                                                                      >  1:191403/1‑123 (MQ=255)                                                                                                                                                                                                 cAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCt                                                                                                                                                                                                <  1:19671/96‑1 (MQ=255)                                                                                                                                                                                                 cAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAANNTGTGCt                                                                                                                                                                                                >  2:19671/1‑96 (MQ=255)                                                                                                                                                                                                      cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGcac                                                            <  1:339599/223‑1 (MQ=255)                                                                                                                                                                                                        gCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAg                                                                                         <  1:35438/192‑1 (MQ=255)                                                                                                                                                                                                          cAACCGGGATTTACACCAACGGTGAGAAGCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTGTATGCATTTAAGGAAAAAAATCTGAGGAAAAGTTGGATATCTGATCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAAc                            <  1:219740/251‑1 (MQ=255)                                                                                                                                                                                                               gggATTTGCACCANCNGGGAGAAGCCACAAACAAAGATGAAAAAAACTTCAAACAGCTATTTGCNGCAGTGTAATNTGTNNTACTNCNANATGACGAAAATAATCAGCTTAATATTTAGNAACATTTGGTGATTAAGTTTTATNNCTNNNATNnaaaaa                                                                                                                   <  2:327479/159‑1 (MQ=255)                                                                                                                                                                                                                                   gaATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAatat    >  1:102805/1‑250 (MQ=255)                                                                                                                                                                                                                                    aaTCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGACATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGttt                                                                                                                                      >  1:382264/1‑119 (MQ=255)                                                                                                                                                                                                                                     aTCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATaa  >  1:4070/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   GCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAA  >  NC_000913/3707712‑3708189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑