Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	13.9 / NA	7	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

GACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCC‑ACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCG  >  NC_000913/3682309‑3682717                                                                                                                                                                                                               |                                                                                                                                                                                                            gACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGcc                                                                                                                                                                 >  1:20908/1‑251 (MQ=255)                                     cccGATACCGGAAAATGGCCCCCCGCGTAGACGGTCGCCGCCAGCACGATAAAGCCACTACCCGTTCCCCCGGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTGGGTGGACGATACCCATCTGATTGTGAGTGGCTTGGGCGAGAAACCCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCC‑ACCACCGATTTACGGCa                                                                                                                            <  1:256914/251‑1 (MQ=255)                                               gaaaaTGGCCACCCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTCCCCTTTCCCCCTGCCCCCCCTTGAGAAGAAAGCACCAACACGATTAACAGCGTGATTGGGTGGACGATATCCATCTGTCGGTTATGGGCCTGGGCGATAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCTCC‑ACCACCTATTTACGGCAGCCGAGTTtc                                                                                                                  <  1:136973/249‑1 (MQ=255)                                                cAAATGGCCAACAGCAGAGAGCGTCGCAGCCAGCACGATAAGGCCACTACCCAATACACTAGCAGCCGGTTTAGACGAAAGCAGATATACGAATACCAGCGTGATGTGGTGTACGGTATCAATCTGACTGTCCGTGGCCTGGGCGATAAGCACCGCAGCTATTGTAAGGTATCTCGAAGCGCCATCAAGGTTAAACGAGTAGCCTGTCGGGCTGACCAGCCCC‑ACCACCGATTTACGGCCGCCCAGTTtct                                                                                                                 >  1:258414/1‑251 (MQ=255)                                                                         gccgccAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGCACGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGCTAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCC‑ACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGc                                                                                          >  1:226383/1‑249 (MQ=255)                                                                                                                         aaaaaaagcaaaaacgaaaTTAACCGCGTTATTGGGTGGGGGATATCCATTGGACGGTTAGTGGCCTGGAAGAGAAACGCCGCCGCTATTGTCAGGTATATCGATGTGCCTTCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCC‑ACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACg                                        <  1:200837/233‑1 (MQ=255)                                                                                                                                                               naCGATATCCATCTGACTGTTAGGGGCCTGGGCGATAAACACAGCCGCTCTTGTCAAGTATATAGATGTGCCATCAAGGTTAACCGAGAAGCCCGTCGGGATGACCAGCCCCCACC‑CAGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGACGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCg  >  1:48768/2‑251 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                            GACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCC‑ACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCG  >  NC_000913/3682309‑3682717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑