Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,626,067	C→T	G246D (GGT→GAT)	yhhJ ←	ABC transporter family protein YhhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,626,067	0	C	T	100.0%	94.4 / NA	33	G246D (GGT→GAT)	yhhJ	ABC transporter family protein YhhJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

CAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACACCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATC  >  NC_000913/3625822‑3626290                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                cAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTc                                                                                                                                                                                                                            >  1:142043/1‑251 (MQ=255)               ggCATGGTCAGCATAATGTCCTGCACCATCTGAGACATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCAcc                                                                                                                                                                                                                >  1:372277/1‑249 (MQ=255)                                               nggCATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCCCCAGAATCACCAGCAGCCCCAGTTGAGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATaccaccagcaccacc                                                                                                                                                                                  >  1:81832/2‑247 (MQ=255)                                               cGGCATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGcc                                                                                                                                                                              >  1:16268/1‑251 (MQ=255)                                               cGGCATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCACCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGcc                                                                                                                                                                              >  1:3211/1‑251 (MQ=255)                                                                                  gCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATaccaccagca                                                                                                                                                                                       >  1:161917/1‑207 (MQ=255)                                                                                        gCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACCCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCAAGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAg                                                                                                                                                                                                              >  1:67561/1‑178 (MQ=255)                                                                                             ggCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCGATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCCGGCCCAGCATAAACAGCGGGATCGAGCCTTCAAGCGGTACGCCCATTACATCTTTCACCATCAGGACCAGCGAGAATCCCGATACCACCAGCACCACCAGCCCCATCGACTAGATCTTCGCCATCATGATCTCAAACGGCGTTATCgg                                                                                                                                <  1:328201/251‑1 (MQ=255)                                                                                                 ncagcaCCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCg                                                                                                                                                            >  1:115093/2‑219 (MQ=255)                                                                                                       ccAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAGTCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGAt                                                                                                                                                                                                          <  1:182596/167‑1 (MQ=255)                                                                                                                          cAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCTATTGACGTGGTGGCAAACAGACTGATCGCCACGCCCAGCATAAACAGCGTGAGCGAGCCTTCAATCGGTACGCCCATTACATCTTTCACCATCAGCTCCAGCGATAATCCCGATaccaccagcacca                                                                                                                                                                                    <  1:383118/170‑1 (MQ=255)                                                                                                                                                                                  cAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTGCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAACCCCGATTCCTCCAGCACCACCAGCCCCATCTACCAGATCTGCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGGTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATcc                                                                       <  1:232721/223‑1 (MQ=255)                                                                                                                                                                                        gACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCTGATCAGCGCCGATc                                                                        <  1:351674/216‑1 (MQ=255)                                                                                                                                                                                        gACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGGAttgttgttg                                     >  1:320839/1‑251 (MQ=255)                                                                                                                                                                                            gAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAgcgc                                                                             >  1:191920/1‑207 (MQ=255)                                                                                                                                                                                            gAGCGCCACGCCCAGCATAAACAGCGGGAACGAACCTTCAATCGGTACGCCCATTACATCTTTCACCATCAGCACCAGCGATAATCCCTATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGc                                                                                                                                                           <  1:142242/129‑1 (MQ=255)                                                                                                                                                                                                       ccAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAgcgcn                                                                            <  1:79019/197‑2 (MQ=255)                                                                                                                                                                                                        cAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGGTATCGGCATCACCAGTAAGTGTTCCAccgtgccgtg                                                                                                  <  1:135632/174‑1 (MQ=255)                                                                                                                                                                                                           naTAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGcc                                                                                                                                                          >  1:277462/2‑115 (MQ=255)                                                                                                                                                                                                           cATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGcc                                                                                                                                                          <  2:277462/115‑1 (MQ=255)                                                                                                                                                                                                             tAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCATTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCTCCACCAGCCCCATCGACCAGATCTGCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGGAAGTGTGCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCAc                       <  1:298275/244‑1 (MQ=255)                                                                                                                                                                                                                aNAGCGGGATCGAGAATTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGAGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCacac             >  1:350267/1‑248 (MQ=255)                                                                                                                                                                                                                     nggATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGt                                                                                                                                      >  1:278207/2‑125 (MQ=255)                                                                                                                                                                                                                     gggATCGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGt                                                                                                                                      <  2:278207/125‑1 (MQ=255)                                                                                                                                                                                                                          cGAGCCTTCANNCGGTACGCCNAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACNAGCCCCATCNNCCNGATCGNCNNNANNNTGNTCTCAAACGGCGTTATCGGNATNNCCAGTANGTGTTCCACCGTGCCGTGTTCACGCt                                                                                          <  2:105011/164‑1 (MQ=255)                                                                                                                                                                                                                          cGAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCt                                                                                          >  1:105011/1‑164 (MQ=255)                                                                                                                                                                                                                           gacccTTCAATCGGTACGCCCAGTACTTCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCTGCATCCCCAGTAAGTGTTCCACCGTGCCTTGTTCACGCTCGTGGATCATCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATc  <  1:382657/248‑1 (MQ=255)                                                                                                                                                                                                                           gAGCCTTCAATCGGTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCAcccc                    >  1:156777/1‑233 (MQ=255)                                                                                                                                                                                                                               cTTCAATCGGTACTCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATaccaccagcaccac                                                                                                                                                                                   <  1:76134/70‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCGGTACGCCCAGTACATCTTTCACCATCAGCCCCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGGAAg                                                                                                                   <  1:128584/130‑1 (MQ=255)                                                                                                                                                                                                                                       ggTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCa                                                                                                                           <  1:26120/118‑1 (MQ=255)                                                                                                                                                                                                                                       ggTACGCCCAGTACATCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCANNATGATCNNa                                                                                                                                              >  2:26120/1‑99 (MQ=255)                                                                                                                                                                                                                                             cccAGTACATCTGTCACCATCATCACCAGCGATAATCCCGATACCCCCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCTCGGTTCAGCGTCGATCCGGTTAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATc  <  1:319621/233‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                CAAAGTGTGTCGTCGGCATGGTCAGCATAATGTCCTGCACCATCTGCGGCATACTTTCGCGCGGCGTGGAACCACCGGAAAGCATTTGCAGCGGCAGCAGCACCAGAATCACCAGCAGCCCCAGTTGCGGCATTGAACGCGCTATCGTCCCCATAAAAATGCCGATTGACGTGGTGGCAAACAGACTGAGCGCCACGCCCAGCATAAACAGCGGGATCGAGCCTTCAATCGGTACGCCCAGTACACCTTTCACCATCAGCACCAGCGATAATCCCGATACCACCAGCACCACCAGCCCCATCGACCAGATCTTCGCCATCATGATCTCAAACGGCGTTATCGGCATCACCAGTAAGTGTTCCACCGTGCCGTGTTCACGCTCGCGGATCAGCGCCGATCCGGTCAATACAATCGCCAGCATGGTAATGTTGTTGATGATCGCCATCACCCCGCCAAACCACGCGGGATC  >  NC_000913/3625822‑3626290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑