Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,586,657	G→A	A56V (GCC→GTC)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,586,657	0	G	A	100.0%	34.4 / NA	14	A56V (GCC→GTC)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

CGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCCCTGCCTGCGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGGCGTCCACAGACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCGCGGCGGCGGCGCTAAAACAACTTCCTGAGAGCAGAGCAGCAATGGCCACCCGGCGTAAAAACGTCGGTTTTATCATCGTTATTCTCCAGAGATTAAGG  >  NC_000913/3586425‑3586845                                                                                                                                                                                                                                         |                                                                                                                                                                                             cGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTACCATAAAACCACCACCGCCCAGATTCACTGCCTGCGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCCTCAACGGCATTCCCGCCCTCCTTGAGACTATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTAcc                                                                                                                                                                             >  1:168097/1‑250 (MQ=255)               caccGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCCCTGCCTGCGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTcgc                                                                                                                                                               <  1:302405/247‑1 (MQ=255)                                                          ncgGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCCCTGCCTGCGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTc                                                                                                                                                                 >  1:281346/2‑205 (MQ=255)                                                               aagcgaaaGCCGTGGTATTACCTTTTTTCGGGCGGTATAACATAAAACCCCCCCCCCCCAGATTCCCTTCCGGCGGATGCATTACCGCCCGCGCATAGCCCACCGCCACGTCGCCATCACCGGCATTCCCGCCCTCCTTGAGAATAGCCACCCCCACCTGAGTGTCAGTGACCTCCCCAGACTCTACCAGTCCCTGTTCCGCGCGTACCGAGGGGAAGACATCTTCCTCCACACCATACGAGACGggcggc                                                                                                              <  1:406853/244‑1 (MQ=255)                                                                                ttGCCATTTTTCGAGCGGATTAACATAAAACCACCCCCGCCCAGATTCCCTGCCTGCGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTcgcgc                                                                                                                                                             <  1:26752/187‑1 (MQ=255)                                                                                                                              cccngccctgcGGATGCGTTCCCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTc                                                                                                                                                                 <  2:281346/131‑1 (MQ=255)                                                                                                                                     ntgcGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCACCGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTCCCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCGCGGCGGCGGCGCTCAAACAACTTCCTGAGAGCAGAGCAGCAATGGCCACCCGGCGTaaa                                         >  1:55270/2‑250 (MQ=255)                                                                                                                                                                  ccACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCCCCTGAGTGGCAGTGACGTCGCCAGACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCGCGGCGGCGGCGCTAAAACAACTTCCTGAGAGCAGAGCAGCAATGGCCACCCGGCGTACAAACGTCGGTTTTCTCATCGTTATTCTcc             >  1:234640/1‑249 (MQ=255)                                                                                                                                                                     ccGCCACGGCGGCATCAACGGCCTTACCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTc                                                                                                                                                                                          >  1:123244/1‑73 (MQ=255)                                                                                                                                                                         cACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTCGCTCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCagg                                                                                                         <  1:383706/150‑1 (MQ=255)                                                                                                                                                                           cggcggCATCAACGGCATTCCCGCCCTCCTTGAGAATAGCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCGCGGCGGCGGCGCTAAAACAACGTCCGGAGAGCAGAGCAGCAATGGCCACCCGGCGTAAAAACGTCGGTTTTATCATCGTTATTCTCCAGAGATTAAgg  <  1:205196/251‑1 (MQ=255)                                                                                                                                                                                  aTCAACGGCGTTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCAACAGACGCTACCATTCCCTGTTTCGCGAGTACCGGGTGGAAGACATCTtcc                                                                                                                                      >  1:137489/1‑112 (MQ=255)                                                                                                                                                                                              cccGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCGCGGCGGCGGCGCTAAAACAACTTCCTGAGAGCAGAGCAGCAATGGCCACCCGGCGTAAAAACGGCGGTTTTatca                         >  1:368777/1‑209 (MQ=255)                                                                                                                                                                                                    ctcctTGAGAATATCCACCCCCACCTGAGTGGCAGTGACGTCCACAGACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCgcggcggc                                                                                             >  1:9461/1‑135 (MQ=255)                                                                                                                                                                                                                                        gnnGTCCACNNACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCgcggcggc                                                                                             <  2:9461/99‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                             CGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGGAAATCGATAGCCGTGGTATTGCCATTTTTCGAGCGGATTAACATAAAACCACCACCGCCCAGATTCCCTGCCTGCGGATGCGTTACCGCCAGCGCGTAGCCCACCGCCACGGCGGCATCAACGGCATTCCCGCCCTCCTTGAGAATATCCACCCCCACCTGAGTGGCAGTGGCGTCCACAGACGCTACCATTCCCTGTTTCGCGCGTACCGGGTGGAAGACATCTTCCTCCACACCATACGAGACGGGCGGCGCAGGAGGCGCGGCGGCGGCGCTAAAACAACTTCCTGAGAGCAGAGCAGCAATGGCCACCCGGCGTAAAAACGTCGGTTTTATCATCGTTATTCTCCAGAGATTAAGG  >  NC_000913/3586425‑3586845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑