Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,563,255	C→T	R415C (CGT→TGT)	glpD →	aerobic glycerol 3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,563,255	0	C	T	100.0%	62.8 / NA	23	R415C (CGT→TGT)	glpD	aerobic glycerol 3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

GAAAATGGCAAAGCACCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGC  >  NC_000913/3563033‑3563441                                                                                                                                                                                                                               |                                                                                                                                                                                           gaAAATGGCAAAGCACCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGCCGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGgcagca                                                                                                                                                                 >  1:103827/1‑250 (MQ=255)    aaTGGCAAAGCACCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGTGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGc                                                                                                                                                                              <  1:330438/234‑1 (MQ=255)                                    nggTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTa                                                                                                                              >  1:129404/2‑250 (MQ=255)                                                  ncTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTCACGCCGTATTATCAGGGTATTGGCCCGGCCTGGAAGACAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACAGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGgcag                                                                                                                                                                   >  1:50242/2‑199 (MQ=255)                                                             cTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCAGGCATGGACGCAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTCTCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCTCACTTACGGCAGCAACCGCGAGCTGCTGCTCGACACTGCGGGAACGGTCACCGCTCTCGGGGACGATTTCGGTc                                                                                                     >  1:308182/1‑250 (MQ=255)                                                                  ggAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTAcn                                                                                                                                                                                   <  1:267549/167‑2 (MQ=255)                                                                   gAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCgg                                                                                                                                      <  1:330125/211‑1 (MQ=255)                                                                            cTGGAAAAACTAACGCCGTATTATCAGGGTATGGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCg                                                                                                                                                                               <  1:259698/161‑1 (MQ=255)                                                                                                              gCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGAtt                                                                                                           >  1:399484/1‑195 (MQ=255)                                                                                                                  ggCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCg                                                                                                                                                                               <  1:362093/123‑1 (MQ=255)                                                                                                                                        cTACCGGGTGGCGCCATTGACGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGAGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCg                         >  1:376650/1‑251 (MQ=255)                                                                                                                                                   cgcCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGACGTGTCATTACGCTCGCACTTACGGCAGAAACAGCGAGCTGCTGCTCGGCACTGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCCCGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACaaacc              >  1:401424/1‑249 (MQ=255)                                                                                                                                                    gcCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAAt                                                                                                                                          >  1:316284/1‑126 (MQ=255)                                                                                                                                                    gcCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAAc                                                                                                                                  >  1:94376/1‑134 (MQ=255)                                                                                                                                                    gcCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCNCCGNCGCCNCTNTCCGTTCCTGACTGANTCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGANNTNNNGNTCGGCAAt                                                                                                                                          <  2:316284/126‑1 (MQ=255)                                                                                                                                                         tGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCTCCGCCGCGATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCCCGCACTTACGGCAGCAACan                                                                                                                                                             <  1:80529/102‑2 (MQ=255)                                                                                                                                                                               gCCGCTCGCCTGCGCCGCCGCTCTCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACtt                                                                                                                                                                          >  1:153608/1‑67 (MQ=255)                                                                                                                                                                                         tGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCTAGCTGCTGCTCGGCAATGCGGTAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAg                                                                                                <  1:297855/131‑1 (MQ=255)                                                                                                                                                                                          gcgcCNACGCTNTNNGTTCCTGACTGAATCGCTGNCGTGACATTACGCTCNCACTNACGgcngcncn                                                                                                                                                               >  2:193597/1‑64 (MQ=255)                                                                                                                                                                                          gcgcCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGTCAGCAACAGCGAGCTGCTGGTCGTCAATGCTGGAACGGTAAGCGATCTCGGGGAAGATTTc                                                                                                         <  1:193597/121‑1 (MQ=255)                                                                                                                                                                                                ccgcTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATgn                                                                                                                                        <  1:88107/84‑2 (MQ=255)                                                                                                                                                                                                ccgcTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGc  <  1:5616/218‑1 (MQ=255)                                                                                                                                                                                                ccgcTATCCGTTCCTGACTGAATCGCTGGCGTGTCATTACGCCCGCACTTACGGCAGCAACAGCGAGCTGCTGCGCGGCAATGCGGGANNGGTAAg                                                                                                                            >  2:5616/1‑96 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                           GAAAATGGCAAAGCACCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAACTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGGACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCTCGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACTTACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGC  >  NC_000913/3563033‑3563441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑