Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,562,125 C→T A38V (GCT→GTT)  glpD → aerobic glycerol 3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,562,1250CT90.9% 42.7 / ‑6.1 22A38V (GCT→GTT) glpDaerobic glycerol 3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/13);  minor base G (0/2);  total (7/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGCTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGTCAGCGAGGCGCTGGCTGAACGTGAAGTGCTGCTGAAAATGGCCCCGCATATCGCCTTCCCGATGCGTTTTCGCCTGCCACATCGTCCGCATCTGCGCCCGGCGTGGATGATTCGCATTGGTCTGTTTATGTACGATCATCTGGGTAAACGCACCAGCTTGC  >  NC_000913/3561883‑3562364
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
gCAGATAAACGCCATAATGGTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGTTTGCGCGa                                                                                                                                                                                                                                         <  1:33615/251‑1 (MQ=255)
  aGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGTTTGCGCGAcc                                                                                                                                                                                                                                       <  1:162734/251‑1 (MQ=255)
                             cACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGTTGGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCAc                                                                                                                                                                                                            <  1:351920/251‑1 (MQ=255)
                                             ttCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCTGTTGCGCGACCTCTTCCGCCAGTTCAAACCTCATTCACGGTGGCCTGCGCTAc                                                                                                                                                                                             <  1:245310/250‑1 (MQ=255)
                                                               gcgATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTTGAGGCGCAGGATCTCGTTTGCGCTACCTCTTCCGCCAGTTCAAAACTCATTGACGGTGGCCTGCGCTACCTTGAGCACTAGTAATTcc                                                                                                                                                                          <  1:376664/251‑1 (MQ=255)
                                                                                                                                         ccAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGCGCTGGTATCGCGGCAGACCACGCTGGCCGCGGCTTATCCGTGATGATGCTGAAGGAGCAGGATATCGTTTGCGTGACCTCTTCCGCCAGTTCAAAACTCATTCAAGGTGGCCTGCGCTCCCTTGAGCACTCTGAAtt                                                                                                                                                                            >  1:211679/1‑175 (MQ=255)
                                                                                                                                           aaaGATCTGAGTGGGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGAGGCTGGAGGCGCAGGATCTCGTTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTgc                                                                                                                                                                                                  <  1:432974/151‑1 (MQ=255)
                                                                                                                                                            aGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGTTTGCCCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAg                                                                                                                                                                                       <  1:289728/145‑1 (MQ=255)
                                                                                                                                                                                cTGGTNNCGNNNCANNCGCCGCTGGACGCGGTTTATCCGNGCTGATGCTGGAGGCGCAGGATCTCGTTTNCNCNACCTNNTCCNCCAGTTCAAANCTCATTCACGGTGGCCTGCGCTACCTTg                                                                                                                                                                                         >  2:328823/1‑123 (MQ=255)
                                                                                                                                                                                cTGGTATCGCGGCAGACGCCGCTGTACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGTGTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTg                                                                                                                                                                                         <  1:328823/123‑1 (MQ=255)
                                                                                                                                                                                        gcgacNNACNNNGCNNGACGCGGTTTATCCGTGCTGATGNTGGAGGCGCAGGATCTCGTTTGCGCGACCNCNTNCGCCNNTTCNAAACTCATTCNCGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGTCAGCGGNGNGCTGGCTGCACGTGAAGTGCTGCTGACAATGNCCCCGCATATc                                                                                                               >  2:338149/5‑189 (MQ=255)
                                                                                                                                                                                        gcgacAGACTCCGCGGGACGCGGTTTAGCCGTGCTGATGCTGGAGGCGCAGTATCTCTTTTGCGCGACCTCTTCCGCCAGTGCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAGCACTAGGAATTCCGCCTGGTCAGCGAGGCGCTGTCTGAACGTGAAGTGCTGCTGAAAATGGCCCCGCATATc                                                                                                               <  1:338149/185‑1 (MQ=255)
                                                                                                                                                                                                cgccgcTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCGCGGTGGCGCGCCCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGGCAGCGAGGCGCTGGCTGAACGTGAATTGCTGCTGAAAATGGCCCCGCATATCGCCTTCCCGAGGCGTTTTCGCCTGCCACATCGTCCGCATCTGCGCCCGGCGTGGATGATTCGCATTGGTc                                         <  1:64472/251‑1 (MQ=255)
                                                                                                                                                                                                            cgGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGTTTGCGCGACATCTTCCGCCCGTTCAAAAc                                                                                                                                                                                                                    >  1:433861/1‑68 (MQ=255)
                                                                                                                                                                                                                   tCCGTNNTGNNNCTNNAGGCGCAGGATCTCGTTTGCGCGNCCTCTTCCGCCAGTTAAAAACTACTTCACNGNGNCCTGNNCTANCTTGAGCACTNTGAATTCCGCCTGGTCAGCGCGGCGCTGGCTGAACGTGAAGTGCTGCTNANCATGGCCCCGCATATCGCCTTCCCGATGCGTNTTGGCCTGCCACATCGTCGGCAGTNGCGCNNgg                                                              >  2:321788/1‑211 (MQ=255)
                                                                                                                                                                                                                          tGATGCTGGNGNNTCAGGATNNCGTTTGNNCGANCTCTTCCGCCAGTTCAAAACTCATTCACGGTGNCCTgng                                                                                                                                                                                                 >  2:364519/1‑73 (MQ=255)
                                                                                                                                                                                                                          tGATGCTGGAGGCGCAGGATCTCGTTTGCGCGACCTCGTCCTCCATTTCAAAACTCATTCACGGTGGCCTgcg                                                                                                                                                                                                 <  1:364519/73‑1 (MQ=255)
                                                                                                                                                                                                                                       gcAGGATCTCGTGTGCGCGACCTCTTCCGCCAGTTCAAAACTCATGCACGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGTCAGCGAGGCGCTGGCTGAACGTGAAGTGCTGCTGAAAATGGCCCCGCATATCGCCTTCCCGATGCGTTTTCGCCTGCCACAGCTTCCGCATCTGCGCCCTGCGTGGATGATTCGCATTGGTCTTTTTATGTACGATCATCTGGGTAAACGCACCAGCTTGc  <  1:321788/251‑1 (MQ=255)
                                                                                                                                                                                                                                           naTCTCGTTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTg                                                                                                                                                                                         >  1:272575/2‑64 (MQ=255)
                                                                                                                                                                                                                                           gATCTCGTTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTg                                                                                                                                                                                         <  2:272575/64‑1 (MQ=255)
                                                                                                                                                                                                                                            aTCTCGTTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGTCATCGAGGCGCTGGCTGAACTGGAAGtgctgc                                                                                                                                   <  1:122456/117‑1 (MQ=255)
                                                                                                                                                                                                                                             tctcGTTTGCGCGCCCTCTTCCGCCAGTTCAAAACTCATGCACGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGTCAGCGAg                                                                                                                                                            <  1:176735/91‑1 (MQ=255)
                                                                                                                                                                                                                                             tctcGNTTGCGNGNNCTCTTCCGCCCGTTCAAAANTCATTCACGGTGGCCNGCGCNACCTTNAGNa                                                                                                                                                                                     >  2:176735/1‑66 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               
GCAGATAAACGCCATAATGTTATACATATCACTCTAAAATGTTTTTTCAATGTTACCTAAAGCGCGATTCTTTGCTAATATGTTCGATAACGAACATTTATGAGCTTTAACGAAAGTGAATGAGGGCAGCATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGCTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAGCACTATGAATTCCGCCTGGTCAGCGAGGCGCTGGCTGAACGTGAAGTGCTGCTGAAAATGGCCCCGCATATCGCCTTCCCGATGCGTTTTCGCCTGCCACATCGTCCGCATCTGCGCCCGGCGTGGATGATTCGCATTGGTCTGTTTATGTACGATCATCTGGGTAAACGCACCAGCTTGC  >  NC_000913/3561883‑3562364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑