Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,506,393	T→C	D315G (GAC→GGC)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,506,393	0	T	C	100.0%	47.3 / NA	21	D315G (GAC→GGC)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/9);  total (12/9)

AATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATG  >  NC_000913/3506197‑3506638                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aaTTGATGCGAAAAGCGATCCGACCAGCGGCCCCCCTTCCTTCAGCATATGCATGGCGTTACGGAGTTTAGCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCAGATCGAACGAAGAAGCAAGACTTCCGCCGAAATAACCTCTTTGCCTAATACCGCTGCAGCCATCTGATTCGGCTAGAGATAGCCCGCCGCATAATCAAAGGTAAAGCCCGCAACTGCATACTCACCCGTTGCTAAc                                                                                                                                                                                                  <  1:325664/250‑1 (MQ=255)        gcGAAAATCGAACCGACCAGCATCGCCACTTCCATCAGCATATTCATGTCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGAGCGAACGAAGCAGCAAGACTTCCGCCGAAATACCCACGGCGCCTAATACCGCTTCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCggtg                                                                                                                                                                                            <  1:310586/249‑1 (MQ=255)                 gAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGAAGCATTACGCACCGCCGGGTAGCGTCCCAGACATTTGACGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATATCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCa                                                                                                                                                                                  >  1:445984/1‑250 (MQ=255)                                                              cGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAATACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTg                                                                                                                                    <  1:306210/251‑1 (MQ=255)                                                                       tCCGACGCNTNNNGNNCCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCNAGAATTCCGCCGAAANAANCACTNCGCNTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTACAGCCCGCAACTGCATACCCACCCGTTGCTACCGCgg                                                                                                                                                                                              >  2:307451/1‑184 (MQ=255)                                                                       tCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCTAGAGATAGCCCACCGAAGAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCgg                                                                                                                                                                                              <  1:307451/184‑1 (MQ=255)                                                                         cGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCAGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCACCGGCACAAACCCCAGTCCGCGCATAAATTCTGCCag                                                                                                                                             >  1:224810/1‑231 (MQ=255)                                                                                             ggTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTTGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGtt                                                                                                     <  1:414952/251‑1 (MQ=255)                                                                                               tAGCGTCGCAGCCATTTGCAGATCGAACGAAGCAGCAAGACATCCGCCGAAATAACAACTGAGACTAATACCGCTGCAACAATCGGATTCGGCGAGAGATAGCCCCCCGCATAAACACAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGAGTTTGCGATTGTTCCGGCGTTAc                                                                                                   >  1:144756/1‑251 (MQ=255)                                                                                                                   gATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCGACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTtct                                                                               >  1:340634/1‑251 (MQ=255)                                                                                                                      cgaacgaaGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGTCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCa                                                                            <  1:414790/251‑1 (MQ=255)                                                                                                                        aacgaaGCAGCGAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGATCCGCCTGATTAATCAGCGTTTGCTATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAgt                                                                          <  1:257792/251‑1 (MQ=255)                                                                                                                         acgaaGCAGCAAGACTTCCGCCGCAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGc                                                                                                                                                                             >  1:156019/1‑151 (MQ=255)                                                                                                                                  caAGAGTTCCGCCGAAATAGCGCCTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAAc                                                                                                                                                                                                                        >  1:119346/1‑99 (MQ=255)                                                                                                                                  caAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTACCGCgg                                                                                                                                                                                              >  1:70541/1‑125 (MQ=255)                                                                                                                                                               cTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAATTGCATACACCCCCGTTGCTAACGCGGCGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTg                                                                                                                                    <  1:405631/154‑1 (MQ=255)                                                                                                                                                                 aaTACCGCTGCAACCATCGGATTCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTTATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAgtg                                                                         <  1:261815/211‑1 (MQ=255)                                                                                                                                                                                       tCGGCGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTACCGCGGTGGTGGCAATCAACGGCAAAAACACCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTACTCAGCGATTGAGATTGTTCCGGCGTTCCGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCt           >  1:395052/1‑251 (MQ=255)                                                                                                                                                                                          ncGAGAGATAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTGATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTccc        >  1:281871/2‑251 (MQ=255)                                                                                                                                                                                            gagagaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGAGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCAc      >  1:381880/1‑251 (MQ=255)                                                                                                                                                                                                gaTAGCCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACTCCCGTTGCTAACGCGGCGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATg  >  1:338605/1‑251 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AATTGCTGCGAAAATCGAACCGACCAGCAGCGCCACTTCCATCAGCATATTCATGGCGTTACGGATGTTATCCGACGCATTACGCACCGACGGGTAGCGTCCCAGCCATTTGCCGATCGAACGAAGCAGCAAGACTTCCGCCGAAATAACCACTGCGCCTAATACCGCTGCAACCATCGGATTCGGCGAGAGATAGTCCACCGCATAAACAAAGGTAAAGCCCGCAACTGCATACACACCCGTTGCTAACGCGGTGGTGGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATG  >  NC_000913/3506197‑3506638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑