Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,492,293	A→G	F2S (TTC→TCC)	ppiA ←	peptidyl‑prolyl cis‑trans isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,492,293	0	A	G	100.0%	35.4 / NA	13	F2S (TTC→TCC)	ppiA	peptidyl‑prolyl cis‑trans isomerase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/5);  total (8/5)

GAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCG  >  NC_000913/3492060‑3492514                                                                                                                                                                                                                                          |                                                                                                                                                                                                                              gAATCATAAAGCCAGGAATGACGCGGTGAAAGGTCGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGTTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTa                                                                                                                                                                                                               >  1:179555/1‑250 (MQ=255)                                              aaaaaCCGCTGTTCACATAATCGACAAAGTTGTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATGCGTGCGGGTCCCCTTGCGCTGGCATGGCGGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCTCCAGGGTCGATTTGGACATGAGATCTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCt                                                                                                                                                                 <  1:261935/251‑1 (MQ=255)                                                                                          ggagctggataaacaaaaagcgccAGTTCGATGTTACAAGCTGAGGTTGTCAACGATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGACAGAGCAGAAAGAGCGAAAACAGCAGCCATAGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGaa                                                                                                                      >  1:388916/23‑250 (MQ=255)                                                                                                      ttATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACATCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTTAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATAGGTAAcc                                                                                                          <  1:380322/250‑1 (MQ=255)                                                                                                                                 gatGAGGTTGTCAACAATACGTGCGTGTCCCCTTTCGCTGCCATTGCGCCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCGCCAGGGTCGCTTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGATATCCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGAc                                                                                                      <  1:322505/225‑1 (MQ=255)                                                                                                                                                           gTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGATCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGATCAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAAttt                                                       >  1:433806/1‑248 (MQ=255)                                                                                                                                                                   tCGCTNNCANNNCTNNGGGAGAAAGAGCAGAAAGAGCGANAACAGCAGCCATCGCCGCCAGGGTCGATTNGnan                                                                                                                                                                                                                             >  2:323542/1‑73 (MQ=255)                                                                                                                                                                                 ncGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAACAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACtt                                                                                              >  1:43289/2‑187 (MQ=255)                                                                                                                                                                                              cagaaagagcGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACACGGCCATCCCTTTACCTNATTNNNNNTannnnnnnnnn                                                                                                                  >  2:273254/1‑144 (MQ=255)                                                                                                                                                                                              cagaaagagcGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATcaca                  >  1:427337/1‑250 (MQ=255)                                                                                                                                                                                                    gagcGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCAGCCCGTTACCTAATTTTACGTAGCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCAc           <  1:67779/251‑1 (MQ=255)                                                                                                                                                                                                    gagcGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCATTCa                                                                                                                                                              >  1:147041/1‑104 (MQ=255)                                                                                                                                                                                                        gAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCACGTGAAGAGAtt                                                                                                                                                                            >  1:382507/1‑86 (MQ=255)                                                                                                                                                                                                             cagcagCCATCGCCGCCAGGGTCGATTTGGACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATcn  <  1:273254/251‑2 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                              GAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGCTGTTCACATAATCGACAAAGTTTTGCACAGACACTGGCGCTTTTTGTTTATCCAGCTCCAGTTCGATGTTACCAGCTGAGGTTGTCAACAATACGTGCGGGTCCCCTTTCGCTGCCATTGCTGCGGGAGAAAGAGCAGAAAGAGCGAAAACAGCAGCCATCGCCGCCAGGGTCGATTTGAACATGAGATTTCCTTAACAAACAGAGCAGAAAAAAAGCAAGTGAAGAGATTCTAAATAGCCTTCAAGAACAAGGCCATCCCTTTACCTAATTTTACGTATCTGAAATATATGTAACCTGACGATCACTTTTTATTCCGTGATCAAAATCACCTCTTAAAATGCAATTTAGCAACCGATTGCAATAAAACATTTAAACAGATCACAAAATCACCTAAAATCG  >  NC_000913/3492060‑3492514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑