Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	303,911	C→T	G91E (GGA→GAA)	ykgJ ←	putative metal‑chelating domain‑containing protein YkgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	303,911	0	C	T	100.0%	29.4 / NA	12	G91E (GGA→GAA)	ykgJ	putative metal‑chelating domain‑containing protein YkgJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

GCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATAGAAGAAGTATCGGGTTTTGTTACCCCTGAAAAACGAAGCCCCGCTATTATCGCTGGCGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTCCGTTCTCACCAGACATGGCGAATTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGG  >  NC_000913/303681‑304079                                                                                                                                                                                                                                       |                                                                                                                                                                         gCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATAGAAGAAGTATCGGGTTTTGTTACCCCTGAAAAACGAAGCCCCGCTATTATCGCTGGCGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATGGc                                                                                                                                                       <  1:257560/250‑1 (MQ=255)          cAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCAGAAGAACGCTAAAGAAGAGAGGCCTTGTTGCTGGCAAATAGAAGACGTATCGGGTTTTGTTACACCTGAAAAAAGAAGCCCCGCTATTATCGCTGGCGGGGCAGGGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCTTCATTGACGACTTCGATCTCACCCGACCTGGCGCATTCTCTg                                                                                                                                             >  1:309115/1‑251 (MQ=255)              gattataTCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATAGAAGAAGTATCGGGTTGTTTTACCCCTGAAAAACTAAGCCCCGCTATTATCGCTGGCGGGTCAGTGCAATTAGTTATTGTATGTGTAGAACTGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGt                                                                                                                                                                       <  1:325879/218‑1 (MQ=255)                                                                             tgCTGGCAAATAGAAGAAGTATCGGGTTTTGTTACCCCTGAAAAACGAAGCCCAGCTATTATCGCCGCGGGGGCAGTGCCCTTAATTATTGCCTGTTAATAACGGCGTAAGACAGTATTTACCCCATGCACGATTGCAAACCTCATTGACGACTTCGCTCTCACAAGACATGGCGACTTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCa                                                                           >  1:239409/1‑250 (MQ=255)                                                                                 ggCAAATAGAAGAAGTATCGGGTTTTGTTACCCCTGAAAAACGAAGCCCCGCTATTATCGCTGGCGGGGCAGGGCAATTACTTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATGGCGAATTCTCTGCCTGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGc                                                                                               >  1:229169/1‑226 (MQ=255)                                                                                         gaagaaGTATCGGGTTTTGTTACCCCTGAAAAACGAAGCCCCGCTATTATCGCTGGCGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATGGCGAATTCTCTGCAGGTGGACGATCTATTTTGATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGt                                                              <  1:393974/251‑1 (MQ=255)                                                                                                   cGGGTTTTGTTACCCCTGAAAAACGAAGCCCCGCTATTATCGCTGGCGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATGGCGAATTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGAt                                                     <  1:236858/250‑1 (MQ=255)                                                                                                                     aaaaaCGAAGCCCCGCTATTATCGCTGGCGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCGCCAGACATGGCGAATTCTCTGCATGTGGACGATCGATTTTTATATAGCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCg                                  <  1:293957/251‑1 (MQ=255)                                                                                                                                                 cGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATGGCGAATTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGAGATTTGTTCAGTGAGc      <  1:345295/251‑1 (MQ=255)                                                                                                                                                     ncAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCAGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGCCTTCGTTCTCACCAGACATGGCGAATTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTAACGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTgg  >  1:42718/2‑251 (MQ=255)                                                                                                                                                                                    ggTGTCAGCCCGTATTTAACCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATGGCGAATTCTCTGGATGTGAACGATCGATTTTta                                                                                                                        >  1:194877/1‑102 (MQ=255)                                                                                                                                                                                             ncGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTTCGTTCTCACCAGACATgg                                                                                                                                                        >  1:80737/2‑61 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                         GCAGATAGTCAGTGAGTATATCGCGCTACTTCAGGATGATGTAGATCCGAAGAACGCTACAGAAGAGAGGCATTGTTGCTGGCAAATAGAAGAAGTATCGGGTTTTGTTACCCCTGAAAAACGAAGCCCCGCTATTATCGCTGGCGGGGCAGTGCAATTAATTATTGTATGTTTATAACGGTGTCAGCCCGTATTTAGCCCTTGCACGATTGCAAGCCTCATTGACGACTCCGTTCTCACCAGACATGGCGAATTCTCTGCATGTGGACGATCGATTTTTATATATCGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGG  >  NC_000913/303681‑304079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑