Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,432,105 C→T L120L (CTG→CTA)  smg ← DUF494 domain‑containing protein Smg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,432,1050CT91.3% 54.8 / NA 23L120L (CTG→CTA) smgDUF494 domain‑containing protein Smg
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/12);  minor base G (0/2);  total (9/14)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAG  >  NC_000913/3431902‑3432317
                                                                                                                                                                                                           |                                                                                                                                                                                                                    
cNGCCCCGCACTTTGGGCAGGACTCCTTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCAt                                                                                                                                                                       >  1:350624/1‑251 (MQ=255)
    cccGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATcc                                                                                                                                                                                                 <  1:239968/221‑1 (MQ=255)
                                                     ttCGCCATAACAACGTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCAGCAGGATCACCCATTTCAGATCATCTAGTGCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCAACTGAATCGGCGCAAGGAAAAGCAGAAAc                                                                                                                   <  1:367514/250‑1 (MQ=255)
                                                               caacTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGGTGTCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGa                                                                                                                                                   <  1:237013/208‑1 (MQ=255)
                                                                 ncTTATGCTGAATTAAAGAAATGATTAATGCAGCATACATTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACCCCATCAGGATCACCCATTTCAGATCATCTCGTTCGAACTCTGCGt                                                                                                                                                                                                      >  1:86037/2‑155 (MQ=255)
                                                                      tGCTGAATTAAAGAAATGATTACTGCCGCATACATTCATTCACTTCAAAGAGTAATTCTTCCATTTGATGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACAATCAGGATCACTCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACt                                                                                                                                                                                     >  1:171309/1‑167 (MQ=255)
                                                                       gCTGAATTAAAGAAATGATTAATGCAGCATCCCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATcc                                                                                                 <  1:364044/250‑1 (MQ=255)
                                                                                     aTGATTAATGCAGCATTCCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTTCGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCCCTATCACCATTTCACGAGTTTCAGGGTTGAGCCCCGGAATCTGCTCAAGGAAAAGCAGAAACCCTCGGCAGCTTGCATCCAGTCGTTCACActct                                                                                  <  1:191088/251‑1 (MQ=255)
                                                                                                  caTACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTCTCCAGCGCGAGCAATCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGa                                                                                                                      >  1:243851/1‑202 (MQ=255)
                                                                                                          nattcaCTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTg                                                                                                                                      >  1:277061/2‑178 (MQ=255)
                                                                                                                  tCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGctg                                                                                                                                                                                <  1:7544/128‑2 (MQ=255)
                                                                                                                                    aTTTGCTGGTACGCATTTGCGCAGCCCGGAATATGGAACAACACCATCAGGATCCCCCAGTTCAGATCATCGAGGTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCAc                                                                                                                                                                  <  1:451861/124‑1 (MQ=255)
                                                                                                                                        gCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCGAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATcn                                 <  1:40078/249‑2 (MQ=255)
                                                                                                                                            gTNNNNNTTTNCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTg                                                                                                                                      <  2:277061/144‑1 (MQ=255)
                                                                                                                                                gCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGAAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATcc                                                 >  1:78120/1‑225 (MQ=255)
                                                                                                                                                  aTTTTCTCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCGAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAAGCTGCTCAAGGAAAAGCAGAAACCCGCGGCAGCTGGCATCCAGTCGTTCACACTCGTTCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCggn                               <  1:86333/241‑2 (MQ=255)
                                                                                                                                                           tcccGGAATATGCAACAACACCATCAGGATCACCCATTTCAGATCATCTAGt                                                                                                                                                                                                                   <  1:257829/51‑1 (MQ=255)
                                                                                                                                                                      tGAACAACACCATCAGGATCACCCATTGCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCACAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTGCTGCCAACCCTGCCTGATAATCAGCAAg  <  1:216396/250‑1 (MQ=255)
                                                                                                                                                                       gAACAACACCATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGt                                                                                           >  1:442923/1‑160 (MQ=255)
                                                                                                                                                                          nancancATCANGATCNCCCATTTCAGATCATNTAGTTCAATCCCGGCGTTGNNCNGGGCGNGCACt                                                                                                                                                                                     <  2:171309/66‑1 (MQ=255)
                                                                                                                                                                           aactccATCAGGATAACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCAttt                                                                                                                                                                     <  1:4375/78‑1 (MQ=255)
                                                                                                                                                                           aacaCCATCAGGATAACCCATTTCCGATCATCTAGTTTGCACTCTGCGTTATCCAGCGCGAGCACTCGCTCTTTCACCAttt                                                                                                                                                                     >  2:4375/1‑82 (MQ=255)
                                                                                                                                                                               ccATCAGGATCACCCATTTCAGATCATCTAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATaa                                                                      >  1:233749/1‑173 (MQ=255)
                                                                                                                                                                                                           |                                                                                                                                                                                                                    
CAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAAAGAAATGATTAATGCAGCATACCTTCATTCACTTCAAAGAGTAATTCTTCCATTTGCTGGTACGCATTTTCGCAGCCCGGAATATTGAACAACACCATCAGGATCACCCATTTCAGATCATCCAGTTCGAACTCTGCGTTATCCAGCGCGAGCACTCGCTCTATCACCATTTCACGAGTTTCAAGGTTGAGCACCTGAATCTGCTCAAGGAAAAGCAGAAACCCACGGCAGCTGGCATCCAGTCGTTCACACTCTTCCGGTGTATAAATACGCATGGAGAGAGGATCCGAGGCCAGTTGCATCGGTTCTGCCAACCCTTCCTGATAATCAGCAAG  >  NC_000913/3431902‑3432317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑