Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	39.7 / NA	15	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/4);  total (11/4)

CCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTG  >  NC_000913/3329512‑3329982                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                ncTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGGGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCggg                                                                                                                                                                                                                              >  1:268367/2‑251 (MQ=255)                      ngcgTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTCCGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGTGGCAAGGCGCCCTGAACCGCTTTACCCTGCCGGGATGCCTGCCACAACGTCATGAGCCGCTCCCGTTGCCTTTTGCCGTGCAGGATGGAGCCACCTATGCCGGTGCATTTCTGAAATATGAGTTAAACAAGGCGGGAATCACCTGGACGGGAACAct                                                                                                                                                                                                         >  1:43493/2‑251 (MQ=255)                                                                                gTTCTGCAGAAGCGCAATACTGAGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGGGGGGAAAAATGCTGCGCCAGACTCCGGTTAACGAAc                                                                                                                                                                               >  1:161764/1‑219 (MQ=255)                                                                                                                              ccTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGATCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGGGCGGGACACTGTTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTTAGATTATGCTGAAAAAGGCGGacaac                                                                                                  >  1:450090/1‑250 (MQ=255)                                                                                                                                 nnccnNNNNTACNCTGACGGGATGCCTGCCACAACGTTCTGGGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACActgc                                                                                                                                                                                                       <  2:268367/143‑1 (MQ=255)                                                                                                                                                    ggATGCCTGCCACACCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTACAACAGGCGGGTGTCAACTGGCGCGGAACACTTCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTTCCAGTAAACCGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAACATCGGGCCACATGATCGCCGATACGGTTTTCCg                                                                           >  1:334756/1‑251 (MQ=255)                                                                                                                                                                                              gCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTCTCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCg                                 >  1:383662/1‑251 (MQ=255)                                                                                                                                                                                               ccGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGAATCCACTGGAGCGGAACACTGCTGCGCCCGACTCAGGTTAAc                                                                                                                                                                                   >  1:182306/1‑104 (MQ=255)                                                                                                                                                                                                 gTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACACTGCTGCGCCCGACTCAgg                                                                                                                                                                                        >  1:226005/1‑97 (MQ=255)                                                                                                                                                                                                    cAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCTCCTGGAGCGGAACACGGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGcc                                                                                                                                               <  1:422468/135‑1 (MQ=255)                                                                                                                                                                                                      ggATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCCGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTCAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATg                                                                                                                    >  1:135766/1‑160 (MQ=255)                                                                                                                                                                                                             gCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCCCTGGAGCGGAACAATGCTGCGCCAGACTCCGGTTAACGAACCTGGACCGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTACGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATACGCGCTTCAAtg                                                 >  1:185248/1‑220 (MQ=255)                                                                                                                                                                                                             gCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACt                                                                                                                                                                                            <  1:4458/81‑1 (MQ=255)                                                                                                                                                                                                                           tGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCCACTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAAAGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCAAGATCTGCTTCAGATTATGCTGAAAAAGTCGGACAAAATGATCGCCGATACGGTTTTTCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACAAGGCGGGCCGGGGAGGACGCCGATCGTCAGAttc    >  1:402086/1‑249 (MQ=255)                                                                                                                                                                                                                             cAATTCTGAAATATGAGTTAAAACAGGCGGGTATCTCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCtn  <  1:129609/251‑2 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                CCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCTG  >  NC_000913/3329512‑3329982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑