Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,050	G→A	Q89* (CAG→TAG)	uxaA ←	D‑altronate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,050	0	G	A	100.0%	14.6 / NA	9	Q89* (CAG→TAG)	uxaA	D‑altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

GTACCTTCGGCGTTGTTGGTCTCTTTCAGAAAACGGTTCTGGATCTGCCGCGCGATGCCGTTGACACAGCCCACGGTTGGCAGGATCCACAGCTCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTG‑AACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTC  >  NC_000913/3242854‑3243193                                                                                                                                                                                                     |                                                                                                                                                 gTACCTTCGGCGTTGTTGGTCTCTTTCAGAAAACGGTTCTGGATCTGCCGCGCGATGCCGTTGACACAGCCCACGGTTGGCAGGATCCACAGCTCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACTTCACGATCTTCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTAATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTG‑AACGTGAACACCTGc                                                                                           <  1:196626/251‑1 (MQ=255)                                                      gATGCCGTTGACACAGCCCACGGTTGGCATGATCCACAGCTCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACGTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGAGAGCGATACTAATCCAGAGCGCTCAGATTCGTGCGCGTAGTGTGGGCGTG‑AACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAAGCGGCAGGCCATATTTGATGACATTgg                                      <  1:175114/251‑1 (MQ=255)                                                                acaccgctcACGGTTGGCAGGATCCACAGCTCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTTCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATTGCGATTCTAATCCAGATCGCTCAGATTCGTGCGCTGATTGTGGGC‑TGTAACGTGAACACCTGCCGCAATATCCTCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGc                            <  1:321552/243‑1 (MQ=255)                                                                              tGGCAGGATCCACAGCTCATTACGCACCCAGACCACGACGTTAGCGCGACGATACCTCTGCACTTCACGACCTGCAGCTTGCGCAGGGAGCTCCTGAAAATCCGGTTGATAGCGATACTAATCCAGATCGCTCAGATTCCTGAGCGTCTTGTGGGCGTA‑AACGTGAACACATGCTGCAATATCCGCCAATGCATAACCACTCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACg              >  1:393598/1‑251 (MQ=255)                                                                                 cAGGATCCACAGCTCATTACACACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTAATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTG‑AACGTGAACACCTGCCGCAATATCCGCCAATg                                                                          >  1:362174/1‑188 (MQ=255)                                                                                          caGCTCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTAATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTG‑AACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTc  <  1:249598/251‑1 (MQ=255)                                                                                              tCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCATGTTGATAGCGATACTAATCCAGATCGCt                                                                                                                                      >  1:178202/1‑116 (MQ=255)                                                                                                   aCGCACCCCGACATCGCCGCTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGAGATCCTAATCCCGATCGCTCAGATTCGTGCGCGTATTGCGGGCGTG‑AACGTGAAAACCTGCCGCAATATcc                                                                                 >  1:182787/1‑163 (MQ=255)                                                                                                                                                               cncAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTAATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTG‑AACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGc             >  1:348853/1‑171 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                 GTACCTTCGGCGTTGTTGGTCTCTTTCAGAAAACGGTTCTGGATCTGCCGCGCGATGCCGTTGACACAGCCCACGGTTGGCAGGATCCACAGCTCATTACGCACCCCGACATCGCCGTTAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTG‑AACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTC  >  NC_000913/3242854‑3243193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑