Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,230,486	C→T	D533D (GAC→GAT)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,230,486	0	C	T	100.0%	73.3 / NA	27	D533D (GAC→GAT)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

GGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGC  >  NC_000913/3230256‑3230719                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ggcaacggcATGCCGGAATATGGCGCGACCCGCGACAACGCACACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGCAACAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGCTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAat                                                                                                                                                                                                                        >  1:389921/1‑250 (MQ=255)  gcaacggcGTGCCGGAATATGGCGCGACCCGCGCCAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGGGAAAAAAGGAGATAAAGAAGAGACGCAGTCTGGGCTGAACAACAACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGGGATGATGCCGCCGGCTTTGGGGTTATCGGTAAAGAACAGTTGGATAAatat                                                                                                                                                                                                                      >  1:324711/1‑251 (MQ=255)               ngAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATggcg                                                                                                                                                                                                         >  1:118126/2‑251 (MQ=255)                               ngcgACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAATAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTg                                                                                                                                                                                         >  1:88275/2‑251 (MQ=255)                                                                        tACGGTAAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGGCAGGaagaacgg                                                                                                                                                 >  1:9799/1‑243 (MQ=255)                                                                          cGGTGAAAAAGGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTAGCGATAAAGAACAGTTGGATAAATATGTCGCTAATggcgg                                                                                                                                                                                                        <  1:157377/193‑1 (MQ=255)                                                                              gAAAAAAGGCGATAAAGAAGAGATGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGCAAACCGCAGTCAGGACGGAACGTTGCTgg                                                                                                                                          >  1:101550/1‑251 (MQ=255)                                                                              gAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTgg                                                                                                                                          >  1:101548/1‑251 (MQ=255)                                                                                aaaaaGGCGATAAAGAAGAGCCGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAAGCGGGGCGGGATGATGCCGCCGTCGTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGGCGCTAATGGCGGCAAACGTAGCGACTTGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGc                                                                                                                                        <  1:291683/251‑1 (MQ=255)                                                                                      gCGATAANGNAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGNCAGTACGACANTCTNNAAATNCNGNCACAGGTTGCTGCATCGTGGGAATCGGGGNGTGATGATGCCGCCGTCTTTGGGNNTANNNATNNAGAAc                                                                                                                                                                                                                                    <  2:335849/153‑1 (MQ=255)                                                                                      gCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGACAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAAc                                                                                                                                                                                                                                    >  1:335849/1‑153 (MQ=255)                                                                                           aaaGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCa                                                                                                                                                               >  1:424284/1‑217 (MQ=255)                                                                                               aagaGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTGATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGGAAACGTAGCGACTTGACGGTGAAATTCGCCGAAAACCTCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAg                                                                                                                         <  1:213628/251‑1 (MQ=255)                                                                                                      gCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAAAGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCgg                                                                                                                  >  1:192701/1‑251 (MQ=255)                                                                                                         gTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCTACTGGACGGTTAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTgg                                                                                                               <  1:307441/251‑1 (MQ=255)                                                                                                            tGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGATGCTGCATCGTGGGCATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGACCGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTgggtc                                                                                                            >  1:436323/1‑248 (MQ=255)                                                                                                                cTGAACCACTACGCCCGCGCGGTGGCGAAAGGCAAGTACGACAGTCTGGAAATTCCGACACAGGTTGATGCATCGTGCGCATAGGGGCGTGATGATGCCGCCGTCCTTGGGTTTATCCATAAAGAACAGTTGGATAAATATGTCGCTCATGGCGGCAATCGTAGCGACTGCACGGTGCAATTCGCAGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCCGTGGATCAGGc                                                                                                        >  1:180552/1‑251 (MQ=255)                                                                                                                  gAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGc                                                                                                        <  1:242667/249‑1 (MQ=255)                                                                                                                            gCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGt                                                                                             >  1:377758/1‑250 (MQ=255)                                                                                                                                                ccNGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCNTGATGATGCNNCCNTCTTTNGNNNTNNNGANAAAGAACAGTTGGATAAATNTGNNGCTAATNGCGGCAAACGTAGCGACTGGACGGTg                                                                                                                                                                                  <  2:96332/145‑1 (MQ=255)                                                                                                                                                ccAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTg                                                                                                                                                                                  >  1:96332/1‑145 (MQ=255)                                                                                                                                                           gTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCACCGTCCTTGGGTTTATCGCTAAAGAACAGTTGGATAAATCTGTCGCTAATGGCGGCACCCGTAGCGCCTGGACGGGGAAATTCGCCGAAAACCCCAGTCAGGACGGAACCTTGCTGGGCTACTCGCTATTGCAGGAGTCGTTGGATCAGGCCAGCTATCTGTACAGCGATAACCATTATCTGGCgtggctggc                                                              >  1:72177/1‑242 (MQ=255)                                                                                                                                                                  aaTTCNNGCNNNGGNNGCTGCATCGTGGGAATCGGGGCGNGATGATGCCGCCGTCATTGGGCTTATCGANANANAACCNNTGGNTCAATATGTTNCTAATGGCGGCAAAAGTAACCACTGGGCGGTGAAACTAGCCGAAAACCNCNACCCGGCCGGAACGTTGCTGTGCTACTCGCCNTTGCCGGAGTAGGGGGAGCCAGCCNGCTANNTGNan                                                                                           >  2:331773/1‑213 (MQ=255)                                                                                                                                                                  aaTTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGTGTTTATCGATAAAGAACAGttn                                                                                                                                                                                                                               <  1:112696/82‑2 (MQ=255)                                                                                                                                                                         gCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTa                                                                             >  1:144118/1‑221 (MQ=255)                                                                                                                                                                             aGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGTCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCa                                           <  1:412585/251‑1 (MQ=255)                                                                                                                                                                                                          gatgatGCCGCCGTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTTGACGGTGAAATTCGCCGAAAACCGCAATCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCATGAGTTGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCTGAGAGTGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTgg               <  1:331773/250‑1 (MQ=255)                                                                                                                                                                                                                      gTCTTTGGGTTTATCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTACCGCCAGTTGGCACAGCAGCTcgc  <  1:161722/251‑1 (MQ=255)                                                                                                                                                                                                                          ntGGGTTNNTCGATAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGa                                                                                                                                                        <  2:9799/96‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          GGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAAGAGACGCAGTCTGGGCTGAACAACTACGCCCGCGTGGTGGAGAAAGGCCAGTACGACAGTCTGGAAATTCCGGCACAGGTTGCTGCATCGTGGGAATCGGGGCGTGATGATGCCGCCGTCTTTGGGTTTATCGACAAAGAACAGTTGGATAAATATGTCGCTAATGGCGGCAAACGTAGCGACTGGACGGTGAAATTCGCCGAAAACCGCAGTCAGGACGGAACGTTGCTGGGCTACTCGCTATTGCAGGAGTCGGTGGATCAGGCCAGCTATATGTACAGCGATAACCATTATCTGGCGGAGATGGCAACCATCCTCGGTAAGCCAGAAGAGGCCAAACGCTATCGCCAGTTGGCACAGCAGCTCGC  >  NC_000913/3230256‑3230719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑