Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,066	G→A	A15T (GCG→ACG)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,066	0	G	A	96.2%	63.9 / NA	26	A15T (GCG→ACG)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (12/13);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCC  >  NC_000913/3207826‑3208293                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    gtgGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAGCCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATc                                                                                                                                                                                                                             <  1:331649/249‑1 (MQ=255)                     agTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAGAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCgg                                                                                                                                                                                                       <  1:96223/251‑1 (MQ=255)                                                     cACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTGTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGctc                                                                                                                                                                       <  1:233667/251‑1 (MQ=255)                                                             caacCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCGCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCGGACGGTCATCGCCAGTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGc                                                                                                                                                               <  1:210677/251‑1 (MQ=255)                                                                        ccGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTAAATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTg                                                                                                                                                    >  1:315720/1‑251 (MQ=255)                                                                          gAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGCGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAggg                                                                                                                                                                                            >  1:64063/1‑209 (MQ=255)                                                                               cAAAAAGCTGTTCGCCGAACGCCGCGATCCCCTCGTGGACGAGATCTGCGAGCACGTCCATTACATCAAATACCCCTCCCGGAGAGGATCACGCCTCTCCTTTTTCGCCGGCATAACACAATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCGCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGtaat                                                                                                                                             >  1:164189/1‑248 (MQ=255)                                                                                             ncGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGATCAACCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGACTGGTGGCGATATCTTGCGCCGCTGACGGTCATCGc                                                                                                                                                                                                                           >  1:82492/2‑159 (MQ=255)                                                                                                   gccgcGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGATCACCCCTCTCCTTTTTCGCAGGAATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACAGGCGTGATCGTTGGACTGATCCTCGAAc                                                                                                                         >  1:303799/1‑251 (MQ=255)                                                                                                     cgcgATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAAccc                                                                                                                       <  1:391843/251‑1 (MQ=255)                                                                                                      gcgATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGGCGGTCATCGc                                                                                                                                                                                                                           <  1:373341/150‑1 (MQ=255)                                                                                                            ccATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCgg                                                                                                                <  1:156663/251‑1 (MQ=255)                                                                                                                         gaTCTGCGAGCACGTCCATTACATCANATAACCNNCCNGGAGAGGCTCACCCCTCTCNTTNNNCNNNGNCATAANACNNTGAAACCTTNCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCNACTTCCGGTNNCCGCAGGGCTGGAGAACCATANCTGNNTNNANTTTNcc                                                                                                                                                              >  2:101854/1‑192 (MQ=255)                                                                                                                         gaTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGCGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATc                                                                                                                                >  1:106957/1‑222 (MQ=255)                                                                                                                         gaTCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGt                                                                                                                                                            <  1:101854/194‑1 (MQ=255)                                                                                                                                   cACGTCCATTACATCAAATAACCCTCCCCGCGAGTCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCCCTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGAGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGGGGCGATGGTgggg                                                                                         >  1:374871/1‑250 (MQ=255)                                                                                                                                                  aaaTAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCATGCATAACACGATGAAACCTTCCTCTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCAGCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGgaga                                                                                                                                                                                     <  1:187652/144‑1 (MQ=255)                                                                                                                                                                    ggCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGTGTGCCGTGGTGGCGATGGTggg                                                                                          <  1:356055/217‑1 (MQ=255)                                                                                                                                                                     gCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGACATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTg                                                                                                                                                                >  1:202408/1‑146 (MQ=255)                                                                                                                                                                             ctctCCTTTGTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCGTGCGCCGCTGACTGTCATCGCCATGATTGCTCTACTTCCGTTTCCCGCAGGGCTGGAGAACCAGTCCTTGCTCTACTTTGCCGGTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGc                                                                                                   <  1:419127/199‑1 (MQ=255)                                                                                                                                                                                         gCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCCCCCTACTCTCTCCCTGGCTGCTGTTc                                                  >  1:140495/1‑236 (MQ=255)                                                                                                                                                                                            ggCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGtttt                                                                                                                                                         >  1:127264/1‑130 (MQ=255)                                                                                                                                                                                                   cacGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGACCCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGCGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCCTACTCTCTCCCTGGCTGCTGTTCCGCCCggcgatcggcagagcccacg                         >  1:385258/1‑233 (MQ=255)                                                                                                                                                                                                                          ggtggCGATATCTTGCTCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCTCTGCAAAATcc  <  1:374303/251‑1 (MQ=255)                                                                                                                                                                                                                          ggtggCGATATCTTGCGCCGCTGACGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATcc  <  1:374311/251‑1 (MQ=255)                                                                                                                                                                                                                           gtggCGAGATCTTGCGCCGCTGACTGTCATCGCCATTATTGCTCTACTTGCGGTTCCCGCAGGGCTGGAGAACCATTCCTGGCTCTATTTTGCCGTGTTTACTGGCGTGATCGTTGGACTGATCCt                                                                                                                              <  1:409765/126‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    GTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCTCTCCTTTTTCGCAGGCATAACACGATGAAACCTTCCACTGAATGGTGGCGATATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCC  >  NC_000913/3207826‑3208293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑