Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,127,435 G→T S196* (TCG→TAG)  glcD ← glycolate dehydrogenase, putative FAD‑linked subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,127,4350GT94.4% 47.2 / ‑5.9 18S196* (TCG→TAG) glcDglycolate dehydrogenase, putative FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (10/7);  total (10/8)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGATTTGTGAGGAAGGGTCCGGTGCGTAGTAGAGATTATGCGGTGCAACGGCCTGGGAGATCGCCAGGTTACGCACGCCTGGCTGCACGCGC  >  NC_000913/3127211‑3127656
                                                                                                                                                                                                                                |                                                                                                                                                                                                                             
aCAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGGCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTcc                                                                                                                                                                                                     <  1:127731/251‑1 (MQ=255)
         ncatcatCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGCTGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGc                                                                                                                                                                                            >  1:45942/2‑251 (MQ=255)
                              ggATAATGCCATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGcc                                                                                                                                                                                                              <  1:399748/212‑1 (MQ=255)
                                                                  gTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCATGCAGTGGAcgccg                                                                                                                                   <  1:135073/251‑1 (MQ=255)
                                                                        cTTTTTCTACCGAGTCAAAGCTGGCTAACAGCACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGCGCATACCTTCCGATCCCGTGAACAGCGCCCGCAGGTCAAAACCCGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGCGCCTCGCCGTCCAGCGCTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGc                                                                                                                              >  1:182624/1‑250 (MQ=255)
                                                                                                      gAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAAc                                                                                                 >  1:139773/1‑249 (MQ=255)
                                                                                                        aCCCGCTCCACGAGCGGCGTCGGCAGCAGTTTGACCGTCACTTCGGTGGTCACGCCTACCATACCTGCCGAACCGGGGAACAGCGCCACCAGGTCAAAACCAGGTGACTCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTcc                                                                                                                                                                                                     <  1:309314/147‑1 (MQ=255)
                                                                                                        aCCCGCGCNANNNGNNGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCNTACCTTCCGATCCGGNGANCAGCNCCANCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTcc                                                                                                                                                                                                     >  2:309314/1‑147 (MQ=255)
                                                                                                           cgcgCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCCTACCTTCAGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGAt                                                                                          >  1:252573/1‑251 (MQ=255)
                                                                                                                  cGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCNTccnn                                                                                                                                                                                                   >  2:271428/1‑137 (MQ=255)
                                                                                                                  cGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCan                                                                                                                                                                                                   <  1:271428/139‑2 (MQ=255)
                                                                                                                        gcTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGATTTGTGAGGAAggg                                                                             >  1:102283/1‑251 (MQ=255)
                                                                                                                             ngcagcaGTTTTACCGTCACTTCGGTGGTCACGCCGAGTATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGATTTGTGAGGAAGGGTCCgg                                                                        >  1:114345/2‑251 (MQ=255)
                                                                                                                                                                  gCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTGCAGCAGGTTATGTACGGTCAGACCATATTTCAGTCAGTTGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGATTTGTGAGGAAggg                                                                             <  1:427620/209‑1 (MQ=255)
                                                                                                                                                                        cTTCCGATCCGGTGAACAGCGCCATCAGGTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCTTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAAtt                                                                                                                                                                                   <  1:376008/101‑1 (MQ=255)
                                                                                                                                                                                                   tgccAAAACCAGGTGAATCCAGCGCGGCCTAGCCAAGCGTCAGTGCCTCGCCGTcc                                                                                                                                                                                                     <  1:141508/53‑1 (MQ=255)
                                                                                                                                                                                                   ggTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCAAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGATTTGTGAGGAAGGGTCCGGTGCGTAGTAGAGATTATGCGGTGCAACGGCCTGGGAGATCGCCAGGTTACGCACGCCTGGCTGCAcgcgc  >  1:295957/1‑251 (MQ=255)
                                                                                                                                                                                                    gTCAAAACCAGGTGAATCCAGCGCGTCCTATCCAAGCGTCAGTGCCTCGCCGTCCAGGGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACAATCTTTCAGGCCGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCCATGGAACAGGCGATTTGTGAGGAAGGGTCCGGTGCGTAGTAGAGATTATGCGGTGCAACGGCCTGGGAGATCGCCAg                           >  1:334156/1‑225 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                             
ACAGGTTATCCATCATCTCCAGCCCGCCGGGGATAATGCCATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGATCCAAGCGTCAGTGCCTCGCCGTCCAGCGTTTGCACTTCAATTTTCAGCAGGTTATGTACGGTCAGACCATATTTCAGGCAGTGGACGCCGCCGGCATTTTCAGCCACATTGCCGCCAATGGAACAGGCGATTTGTGAGGAAGGGTCCGGTGCGTAGTAGAGATTATGCGGTGCAACGGCCTGGGAGATCGCCAGGTTACGCACGCCTGGCTGCACGCGC  >  NC_000913/3127211‑3127656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: