Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,076,531	G→A	T309M (ACG→ATG)	yggP ←	putative zinc‑binding dehydrogenase YggP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,076,531	0	G	A	100.0%	43.9 / NA	17	T309M (ACG→ATG)	yggP	putative zinc‑binding dehydrogenase YggP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/6);  total (11/6)

GCCTTTTTCGGCAAAATCGGCAATGGCAGTGAGCGGCATGGTCACGCCGTTATAAATGAGTTTTTTACCGCCAGGGATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGG  >  NC_000913/3076305‑3076724                                                                                                                                                                                                                                   |                                                                                                                                                                                                  ttccgatcgggCAAAGTCGGCAATGGCAGTGAGCGGCATGGTCACGCCGTTATAAATGAGTTTTTTACCGCCAGGGATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCGGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAg                                                                                                                                                                            <  1:135492/241‑1 (MQ=255)  ccTTTTTCGGCAAAATCGGCAATGGCAGTGAGCGGCATGGTCACGCCGTTATAAATGAGTTTTTTACCGCCAGGGATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGa                                                                                                                                                                           >  1:260647/1‑250 (MQ=255)  ccTTTTTCGGCAAAATCGGCAATGGCAGTGAGCGGCATGGTCACGCCGTTATAAATGAGTTTTTTACCGCCAGGGATATCCGGCAGATTAAGCAAGGTTTCTGGAACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGa                                                                                                                                                                           >  1:298223/1‑250 (MQ=255)                                       tGGTCACGCCGTTATAAATGAGTTTTTTACCGCCAGGGATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCg                                                                                                                                     >  1:200182/1‑251 (MQ=255)                                                                    ccGCCAGGGATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGGTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGc                                                                                                        <  1:186229/251‑1 (MQ=255)                                                                          gggATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATACACGACGGATGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCCTGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCAGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTa                                                                                                   >  1:329428/1‑250 (MQ=255)                                                                                                         gCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCAcc                                                                   >  1:9362/1‑251 (MQ=255)                                                                                                                ntCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAAATGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTAACGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAg                                                                                                     >  1:80630/2‑210 (MQ=255)                                                                                                                   cAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATaa                                                          >  1:13948/1‑250 (MQ=255)                                                                                                                         aCCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAGTCGCCTCTTTCGTGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTCGACATTAAACGGCACTTTGTCGTTTTTATCCGTAGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACaaaa                                                    >  1:447662/1‑250 (MQ=255)                                                                                                                                tGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTAATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGAACTTTGAAGTTTTTATc                                                                                                                                               >  1:36280/1‑152 (MQ=255)                                                                                                                                                   gCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTc                         <  1:377339/251‑1 (MQ=255)                                                                                                                                                   gCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTGTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTc                         <  1:25209/251‑1 (MQ=255)                                                                                                                                                                     cgcaaaGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGGCGACGTGCATGCGGTGGTAATGGACGTTGTAGAAATTAAACAGCACTTTTAAGTTTTTATCCGGCTGCCCGGCAAAGAAGTCCAGACAGCCTTCTTCCGCCAGGAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCg                                           <  1:236931/211‑1 (MQ=255)                                                                                                                                                                       cTAAGGGCAATCGCATCTTTCATGTAGTCCGTTCAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGACGTTTTTATCCGTc                                                                                                                                           >  1:121470/1‑117 (MQ=255)                                                                                                                                                                          aGGGCAATCTCCTCTTTCATGTCGTCCGTTGAACCGCCAAATGTACCGACGACGTGCATGCTGTTGTAATGGACGGTGTAGAAATTAAACGGCACTTTGAAGATTTAATCCGTCGGCCCGGCAAAGAAGTTCAGACAACCATCTTCCGCCATTAATTGATCAGCCATCTCAACGACAGCTGGCGCCGCCGCATAAACAAAAATGTCATCGATCCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAgg  <  1:432081/251‑1 (MQ=255)                                                                                                                                                                                                 gtcCGTTGAACCGCCAGATGTACCGACGACGTGCATGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATc          >  1:137681/1‑220 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                  GCCTTTTTCGGCAAAATCGGCAATGGCAGTGAGCGGCATGGTCACGCCGTTATAAATGAGTTTTTTACCGCCAGGGATATCCGGCAGATTGAGCACGGTTTCTGGCACCGCATCCAGGCCACCGATATGGGTCACCATAAACGACGGCTGTAACTGCCCAGTGGCGCTAAGGGCAATCGCCTCTTTCATGTCGTCCGTTGAACCGCCAGATGTACCGACGACGTGCGTGCTGTTGTAATGGACGTTGTAGAAATTAAACGGCACTTTGAAGTTTTTATCCGTCGGCCCGGCAAAGAAGTTCAGACAGCCATCTTCCGCCAGTAATTCATCAGCCATCTCAACGACAGCAGGCACCGCCGCATAAACAAAAATGTCATCGAACCCGGCATCTCCTGTCAGCGCCCGCAGCATCTGGACAGG  >  NC_000913/3076305‑3076724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑