Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,827	C→T	Q281* (CAG→TAG)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,827	0	C	T	100.0%	21.9 / NA	10	Q281* (CAG→TAG)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

CCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGAAAAACGTGGAATCGATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCTAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGT  >  NC_000913/3063604‑3064047                                                                                                                                                                                                                                |                                                                                                                                                                                                                             ccATACCAATGTCGCCATTGTCCGGCATATGTAGGAGAGTGCCCTGCCGATTCTGTGAAGTAAATACGACGAAGGGAAGCCACCGGGTCTAACGTGTAGAGCCCTGGAAAAACGGGGAATCGAAGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCTAACTGCCATTGGTCGTCTACGATAAGTGCGGCAACAACAATCTGTGGAAGGGCTGCGTAAGTACACCTAAGAAGAAGTACTGAATCAcc                                                                                                                                                                                                   <  1:452804/251‑1 (MQ=255)      ccAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCACTGCATATTCTGAGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGAGCACTGGAAAAACGTGGAATCGATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCTAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAAGAAGCAGTACTGAATCACCTGTTc                                                                                                                                                                                              >  1:331317/1‑251 (MQ=255)                                     gagTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGAAAAACGTGGAATCGATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCGAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAATAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCg                                                                                                                                                                              <  1:318475/236‑1 (MQ=255)                                                        cGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGAAAAACGTGGAATCGATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCTCTAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGaa                                                                                                                                                                                     <  1:224857/210‑1 (MQ=255)                                                                                                                          gATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCTAACTGCCAGTGGTCGATTACAACAAGTGCGGCACCAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGCTCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATAttt                                                                          >  1:409484/1‑251 (MQ=255)                                                                                                                                        ngCCATCATCGACTTCAAAACCGCGCTAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAAtt                                                            >  1:112277/2‑251 (MQ=255)                                                                                                                                                      tCAAAACCGCGCTAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCTGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATc                                               <  1:203869/250‑1 (MQ=255)                                                                                                                                                                                      caacaaGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGTAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTgg              >  1:383233/1‑251 (MQ=255)                                                                                                                                                                                                  caacaacaaTCGGTGGAATGGCTGCGTAAGTAGACCGACGAAGAAGTACTGACTCACCTATTCGAGCGTGCAGATTTCGATCGATATTACCGCCAGACGCTTTTAGCGGTCAAAAACACTACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCACCAAAGTGGCGGTCATTGAgg  >  1:63777/1‑250 (MQ=255)                                                                                                                                                                                                                       cttcGTAAGTAGACCGAATAAGAAGTACTGAAGCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCATTGAATTTAGCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATcaaca                    <  1:402386/209‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                             CCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGAAAAACGTGGAATCGATGAGATCTGGCACGCCATCATCGACTTCAAAACCGCGCTAACTGCCAGTGGTCGTTTACAACAAGTGCGGCAACAACAATCGGTGGAATGGCTGCGTAAGCAGACCGAAGAAGAAGTACTGAATCACCTGTTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGT  >  NC_000913/3063604‑3064047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑