Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,1330CT94.7% 43.2 / ‑3.5 19S211F (TCT→TTT) nrdFribonucleoside‑diphosphate reductase 2 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (7/11);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

GCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCC  >  NC_000913/2803894‑2804364
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       
gCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGCTGATCCGCTGAAAAAGAAAATCGCCAGAGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATTCCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAAc                                                                                                                                                                                                                              >  1:434227/1‑251 (MQ=255)
 cAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACg                                                                                                                                                                                                                             >  1:343231/1‑251 (MQ=255)
      aaaaTCTCAGATTATTCAGCAACATTATCGCGGTTATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTGTTTGTTCTATTCCTGTTTCTTGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGCCCTGATCCGTCTTATTATCCGCGATGAAGCAGTCCACGGTTTCTACATAGTCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGTGACAACTtgaag                                                                                                                                                                                                                        <  1:343515/251‑1 (MQ=255)
                                                gCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGCGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTAcn                                                                                                                                                                              <  1:87703/251‑2 (MQ=255)
                                                                                                                     gTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCGATAAAGATCAGAAAAACATGGAAAATATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCg                                                                                                                                                                                                         <  1:286890/155‑1 (MQ=255)
                                                                                                                           ttCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAGCATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCAACGACAACTAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGt                                                                                                    <  1:435469/250‑1 (MQ=255)
                                                                                                                             ccAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGAGGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCGGGGACAACg                                                                                                                                                                                                                             <  1:364288/127‑1 (MQ=255)
                                                                                                                                 ccGCTGAAAGCTGACCAATACCGCTGACCTTATCCGTCTGATTATCCGCGATGAAGCAGGCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACg                                                                                                                                                                                                                             <  1:305861/123‑1 (MQ=255)
                                                                                                                                 ccGCGGAANGNNNANNAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTNCACGGTTACTACATANGCNATAANTATNAGAAAAACATGGAAAAGATATTTCTGGGACAACg                                                                                                                                                                                                                             >  2:305861/1‑123 (MQ=255)
                                                                                                                                   gcgGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATttgttg                                                                                                                                                                                           >  1:32495/1‑155 (MQ=255)
                                                                                                                                             gACCAATACCGCTGACCTGATCCGTCTGATTATCCGCTATGAAGCAGTCCACGGTGACTACATATGCTATAAAGATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATttg                                                                                                                                                                                              <  1:226806/142‑1 (MQ=255)
                                                                                                                                                                             gccGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTGCGATGTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGACTCTGGTCTACGAACCGGTATTTccc                                                 <  1:380260/250‑1 (MQ=255)
                                                                                                                                                                              ccGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGAGTTGTTGCTGGAACTCTACGACAGCGAGTTGCAATACACCGAGGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCGCTAAAGCTTTGATGAATCTGGGCTACGAACCGTTATTTccc                                                 <  1:380262/250‑1 (MQ=255)
                                                                                                                                                                                     gAAGCAGTCCACGGGTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTTGGCTACGAACCGTTATTTCCCGCAGAAAt                                         >  1:158603/1‑251 (MQ=255)
                                                                                                                                                                                                      ntacATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGc                                                                                                                             >  1:130352/2‑150 (MQ=255)
                                                                                                                                                                                                                          aGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTGTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCATTACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCg                                     <  1:35371/218‑1 (MQ=255)
                                                                                                                                                                                                                            aaaaaCATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTTAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAAGACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTTAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGGGACGGACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGcn  <  1:44146/251‑2 (MQ=255)
                                                                                                                                                                                                                                     gAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAAt                                                                                                                                                               >  1:356155/1‑85 (MQ=255)
                                                                                                                                                                                                                                          gATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTcgc              >  1:357035/1‑225 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                       
GCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCC  >  NC_000913/2803894‑2804364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑