Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,630,852 G→A S176N (AGC→AAC)  yfgI → nalidixic acid resistance protein YfgI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,630,8520GA95.8% 64.0 / NA 25S176N (AGC→AAC) yfgInalidixic acid resistance protein YfgI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/10);  minor base C (1/0);  total (14/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATG  >  NC_000913/2630612‑2631077
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 
aaCGCGCTCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCACGCGTTAAGCGAGAAAACGAATTTATCGATCAGGCACTGAAGCACAAAGATGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTttaat                                                                                                                                                                                                                         >  1:232801/1‑251 (MQ=255)
     gATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAACAACTGGCGCTGGCGAAAGAGCAAGCCCGAGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGATGCGCAAACAGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGtt                                                                                                                                                                                                                             >  1:207013/1‑242 (MQ=255)
                         ncTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTAGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATcc                                                                                                                                                                                                >  1:280153/2‑251 (MQ=255)
                                                                              cccGCGTTAAGCGAGAAAACTAATTTATCGAGCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACGGAAGGCGGTTGCGATCTTATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTtta                                                                                                                                                                                                                           <  1:170821/171‑1 (MQ=255)
                                                                                   gTTAAGCGAGCAAACGAATTTATAGATCAGGAACTGAAGAACAAAGCTGCGCAAACAGATGTGGTGAAATCTGAAGCTGCCGCCAACCGAAATATGACTGAAGGCGGACGCGATCTGATGAAAAGCGTGGGCACAGCAGAAGCGAACCAATCGGACAACTGGTTTAATTAATAGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGAAGTAACAGAGTGTTTTGTtcattcat                                                                                                                                        >  1:71390/1‑249 (MQ=255)
                                                                                   gTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATAACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCCAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAAAAGAGGGTTTTGTtccttcat                                                                                                                                        >  1:71371/1‑249 (MQ=255)
                                                                                               aaCGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCACAAACCGATGAGGTCCAATATGCAGCTGACGCCAACAGAAATATTACTGAAGGCGGTCACAATCTGATGAAAAGAGTGGGCAAAGCAGAAGCGACCAAATAGGACACCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATCATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaa                                                                                                                           >  1:171039/1‑250 (MQ=255)
                                                                                                   aaTTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCGGAAGCTGACGCCAACAGAAATGTGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATTGGACATCGGGTTTAATTAATCGATGTTAGTAGCTTCAGTCCTATAATTCTGGAAGAGTAAAAACCCTCTGTAGGATCTGAGGGTTTTGTTCATTCATAGTGCAGGTTCAAATCAt                                                                                                                      <  1:173265/251‑1 (MQ=255)
                                                                                                                                        aaCCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTGTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGtttt                                                                                                                                                  <  1:184910/186‑1 (MQ=255)
                                                                                                                                               gtggtgCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTggcn                                                                                                <  1:51533/229‑2 (MQ=255)
                                                                                                                                                tggtgCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCtnta                                                                                                                                                                                            >  2:274051/1‑136 (MQ=255)
                                                                                                                                                    gCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCAcgc                                                                     <  1:95712/251‑1 (MQ=255)
                                                                                                                                                       aTCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGAGAAAAAACCCTCTGTAGTAAc                                                                                                                                                            <  1:371702/161‑1 (MQ=255)
                                                                                                                                                                                           nnnnnnnnnncTNNNNNAAANCGTAGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAggg                                                                                                                               <  2:280153/144‑1 (MQ=255)
                                                                                                                                                                                                 cnANNTNNTGANAAGCGTGGGCAAAGCAGAAGANNACAAATCGGNCAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGANAAAAAACCCNNTGNAGTAANANNNGNNNTTNTTCATTCATAGTGCAGGGTNAANNCATTCCNACTCAATGGTAGCTGGCGGCTTGCCg                                                                                        <  2:104780/187‑1 (MQ=255)
                                                                                                                                                                                                 cgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTACTTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCg                                                                                        >  1:104780/1‑187 (MQ=255)
                                                                                                                                                                                                  gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCANTCANNNNNCannnnnn                                                                                                                            >  2:267984/1‑144 (MQ=255)
                                                                                                                                                                                                                cGTGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCTATGGTAGTAACTTCAATc                                                                                                                                                                                                 <  1:127740/67‑1 (MQ=255)
                                                                                                                                                                                                                 gTGGGNNAANNNGANNAGAACAAATCGGACAACTGGTTTNATTAATCGATGTTAGTAACTTCAATCCTANANTNCTTGNNGATNAAAAACCCTCNgtagta                                                                                                                                                              >  2:325261/1‑101 (MQ=255)
                                                                                                                                                                                                                  tGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgc                           >  1:139046/1‑231 (MQ=255)
                                                                                                                                                                                                                  tGGGCAAAGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGATAGTAACTTCAATCATATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGTTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACTGAGGAAATCGTAc          >  1:15167/1‑248 (MQ=255)
                                                                                                                                                                                                                       aaaGCAGAAGAGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATg  <  1:145150/251‑1 (MQ=255)
                                                                                                                                                                                                                              cagagATCAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCCCGCGGGAAATACAGTTCACTTCATTGATAATGCGGTTGGACACGCGACCGAGGAAATCGTACGGCAGa    >  1:119131/2‑242 (MQ=255)
                                                                                                                                                                                                                              aagaGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCANTCannnnn                                                                                                        >  2:280624/1‑137 (MQ=255)
                                                                                                                                                                                                                              aagaGAACAAATCGGACAACTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAtn                                                                                  <  1:280624/164‑2 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 
AACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAACTGAAGCACAAAGCTGCGCAAACCGATGTGGTGCAATCTGAAGCTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTACGGCAGATG  >  NC_000913/2630612‑2631077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: