Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,473,392	G→A	intergenic (‑146/‑212)	yfdO ← / → yfdP	protein YdfO/uncharacterized protein YfdP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,473,392	0	G	A	100.0%	34.9 / NA	13	intergenic (‑146/‑212)	yfdO/yfdP	protein YdfO/uncharacterized protein YfdP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

GCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATT‑TT‑ACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGATG  >  NC_000913/2473178‑2473628                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               gCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTCACggg                                                                                                                                                                                                           <  1:102995/251‑1 (MQ=255)                     gCTCGTACAGGGCGATGCTTTTTTTCCAGAGCCACTGTGAGCAGGTCAAATCATCATGCGTTCACCACTGGCGCTTTTTAGGGCTGAATACAACCGCATACGGATGGCGTGTTAAAAAATCCTGTTCCTCCGTCTGCGTGTCCGGTTGCGAAGCGTGCGGACGAGAAGGTTTTTTATCTACCGGAACATGTTTTAATT‑TTTACTGCCGGATCCACGCCAGATTCTGACGGGTGAAAACCCAATTTTTTGcc                                                                                                                                                                                       >  1:293753/1‑251 (MQ=255)                                              ccAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCACACTGGCGCTTTTTCGGGCTGACTACAACCGCATCAGGATGCCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATc                                                                                                                                                                                                                              >  1:188216/1‑187 (MQ=255)                                                           gcgcAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGAtttt                                                                                                                                                  <  1:207423/250‑1 (MQ=255)                                                                             tGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCTAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGAGCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCATATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGAc                                                                                                                                <  1:191701/250‑1 (MQ=255)                                                                               cGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACggg                                                                                                                             <  1:213099/251‑1 (MQ=255)                                                                                           gcgcTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGTGa                                                                                                                                                                                                        >  1:324677/1‑164 (MQ=255)                                                                                           gcgcTTTTTAGGGCTGAANANAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCTTCTGCGTNTCCGGTTGCGNAGCNNCCGGNCNANAAGGTTTTTTATCTGACGGATCATGTTTTNATT‑TTTACTGACGGATCCCCGCCNNATNNNGANNGGTGa                                                                                                                                                                                                        <  2:324677/164‑1 (MQ=255)                                                                                                                         aGGATGACGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTAGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGTTCATGTTTTAATT‑TTTCCAGACGGATCTCCGCCAGCGTCTGACGGGTAAACACCCGATTTTTTGCCAGATTTCGCCGCATCAAATTTTGACTGGTCAGATTTTGATCCGTCGGATTTTGACGGGTCAGAGTCTGAAAGTTGAGAAAATGCCGCTGCCTGAAGCTTc                                                                                   >  1:133806/1‑251 (MQ=255)                                                                                                                                                             cGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCgg                                               <  1:33924/251‑1 (MQ=255)                                                                                                                                                                        ncGNAGCGTNCGGANGAGAAGGTTTTTTATNTGACGGATCATGTTTTAATNNTN‑ACTGTNGGATc                                                                                                                                                                                                                              <  2:188216/64‑1 (MQ=255)                                                                                                                                                                        gCGAAGCGTGCGGACGAGAAGGTGTTGTATCGGACGGATCAGGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTGCGACGAATCAAATGTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCGTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                    <  1:404476/251‑1 (MQ=255)                                                                                                                                                                                           aGGTTTTTTATCTGACGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTGTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCg                 <  1:225881/251‑1 (MQ=255)                                                                                                                                                                                                          cGGATCATGTTTTAATT‑TTTACTGACGGATCCCCGCCAGATTCTGACGGGAGAAAACCCGATTTTTTGCCAGATTTCGAAGCATCAAATTTTGACGGGTCAGATTTTGCTGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAACTGGCTAGCGAACGCAGTACTGAtg  >  1:313777/1‑251 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               GCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATT‑TT‑ACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGATG  >  NC_000913/2473178‑2473628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑