Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,392,233	C→G	G270A (GGT→GCT)	nuoM ←	NADH:quinone oxidoreductase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,392,233	0	C	G	100.0%	40.1 / NA	19	G270A (GGT→GCT)	nuoM	NADH:quinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/10);  total (9/10)

GACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGAGTGCGCATCCGGCAGCCAGCCATGCAGCGGAACC  >  NC_000913/2391990‑2392331                                                                                                                                                                                                                                                    |                                                                                                   naCAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGCTAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCAGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGc                                                                                             >  1:47987/2‑251 (MQ=255)                            cccGCGCCCTGGTAGGCCAACTGGCTGCCGGTTTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCTATGTGTAGGCGATCAGACGGTTGATATCGGTCTGGGCGAAGGCCCTCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCATGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAg                                                                  <  1:330281/250‑1 (MQ=255)                            aCCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGTCAGTGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAg                                                                  <  1:330299/251‑1 (MQ=255)                            aCCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAAACAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCTAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAAGTGGCGCGAACTCTGCCGACGCGTTCGTGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCAGCGAg                                                                  <  1:330280/251‑1 (MQ=255)                                  cccTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTc                                                                                   >  1:13538/1‑228 (MQ=255)                                           gCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGCTGTCGGCGCTCAGACGTTTGATATCGGTCTGGGCGCAGGCCATCCACGCACCGTAGAAGATGTCGATAACACCCCGCCACATAGCAATTGGCTCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACcggc                                                   >  1:216120/1‑251 (MQ=255)                                                  ggCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGCTCAGACGTTTGATATCGGACTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATCGCAATTGACGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCggg                                            >  1:392125/1‑250 (MQ=255)                                                         ggTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATCGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTggg                                     >  1:395835/1‑251 (MQ=255)                                                                  aGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGAAAACACCCAGCCACATATCAATGGTCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTGTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGAGTGCGCa                             <  1:446254/250‑1 (MQ=255)                                                                   gCAATCAGCACGAAGCCCCTGTGGGACACCGAGGTGTCGGCGATCAGACGTTTGAAATCGGTCTGGGCGACCGCCATACCCGCACCGTAGCAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGCACAGCGGCCGGCAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCACGATCCCCGCGAGGTCCACGGAACCGGCGGCCGGAGccagagaggaaaagc                            >  1:397725/1‑237 (MQ=255)                                                                                 gCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGGCGGAGCCCGGGGGTGGGCATCCGGCAgccagcca              >  1:150248/1‑250 (MQ=255)                                                                                           gAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGTAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAGCTCTGTCGACGCGTTCGGTAACAGCGGCAGGTAGAGACCCATCAAAGCGTAAGTTGCAGTTTGCAGCAAGATCCCCGCGAGGTCAACGGAACCTTTGGTCGGATCCTGGGAGTGCGCATCCGGCAGCCAGCCATGCAGCGGAac   <  1:221534/251‑1 (MQ=255)                                                                                            aaaCCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGGATGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGCCAACGGAACCGGTGGGCGGATCCAGGTAGTGCTCATCCGGCAGCCAGCCATGCAGCGGAacc  <  1:404897/251‑1 (MQ=255)                                                                                                                                     gCGAAGGCCATCCACGCACCGTAGAAGNTNCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGNTCGGGAACAGNGGCNNGGAGNANCNCAGCAAAGCGTAAGCGGCAGTTTTCAGCANGATCCCCGCGAGGTCAACGGAACNNGCNNNCGNNGCCTg                                       <  2:331654/173‑1 (MQ=255)                                                                                                                                     gCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGAAGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCCGAGACTg                                       >  1:331654/1‑173 (MQ=255)                                                                                                                                                                              cAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGAGCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGAGTGCGCATCCGGCAGCCAGCCATGCAGCGGAac   <  1:158953/168‑1 (MQ=255)                                                                                                                                                                                        gCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAAGCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGAGTgcg                               <  1:386282/130‑1 (MQ=255)                                                                                                                                                                                                                                     gagaANCGCAGNANNGCGTAAGCCGCAGTTTTCANCAAGATCCCCGCGAGNTCAANGGAACngtngn                                                 >  2:180524/1‑63 (MQ=255)                                                                                                                                                                                                                                     gagaAACGCAGCAAAGCGTAAGCCGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACTGAACCGGCTGTCGGAGCCTGGGAGTGCGCATCCGGCAGCCAGCCATGCAg         <  1:180524/107‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                   GACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAACACCCAGCCACATAGCAATTGGCGCGAACTCTGCCGACGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGAGTGCGCATCCGGCAGCCAGCCATGCAGCGGAACC  >  NC_000913/2391990‑2392331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑