Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,319,078	C→A	D267Y (GAT→TAT)	rcsC ←	histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,319,078	0	C	A	100.0%	50.5 / NA	18	D267Y (GAT→TAT)	rcsC	histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/9);  total (9/9)

CGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTAT  >  NC_000913/2318832‑2319314                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             cGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATGTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGccc                                                                                                                                                                                                                                          <  1:155449/251‑1 (MQ=255)     gCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTa                                                                                                                                                                                                                                       >  1:101450/1‑250 (MQ=255)                gAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTCAACAATGCAGCCCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCCCCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCt                                                                                                                                                                                                                           >  1:203997/1‑251 (MQ=255)                                      cATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGATGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAggg                                                                                                                                                                                                     <  1:32584/251‑1 (MQ=255)                                                                               tCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCTCCTTATCAACCGGCACCGAATACACGATGCTGAGCGATGAGGGTGGCAGATTTGTCTTCAGCACCAGCTCCCGGAACCCGTCCGTATAGCCAAACCAGGAGCTTTCCGGCAGCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTa                                                                                                                                                                                                   <  1:252129/212‑1 (MQ=255)                                                                                                                   gTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATg                                                                                                                           <  1:3467/248‑1 (MQ=255)                                                                                                                       cAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACt                                                                                                                                                                                                                               <  1:197851/144‑1 (MQ=255)                                                                                                                                          aCCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCTCAAGCTCCCGGAACCCTTCAGAATAGACACAACAGGAGCGTTCCTGCATCCAGCGCGAATAGCCCTTAATTTTACTTTCTGGACCGGAAAGCGAAATCAGGGAATGACCATTTTCATCAAGAATGGTAACCCCCATCGGAAACGAACCCGGTAAGAAAAAGTTCTCAATCCGGGTGGTCGGCTCGACACCCAAAAGCGCCTg                                                                                                 >  1:319623/1‑251 (MQ=255)                                                                                                                                                     gATGCTTCGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGCTCGacac                                                                                                              >  1:145265/1‑227 (MQ=255)                                                                                                                                                                                  nctncAGNANNANNTCCNGGAACCCTTCCGTATAGCCAANNCAGGAGCGTNCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAANTCAGGGTATNNCCNTTTTCNTNNNGNNNGGNAACCCCCATCGGCAACGTANCCNNTAAGAANAAGTTCTCCATCCGGATGGTCTGCTc                                                                                                                   <  2:101450/192‑1 (MQ=255)                                                                                                                                                                                                                   ngCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTCGTCCGGAAAGCGAAAACAGGGTAAGAACCTTTTCCTCACGAATCGTACCCCCCATCGGCAACGTCCCCGGTCAGACAAAGTTCTCCATCCCGCTGGTCTGCTCGCCAACCAAAAGCGCCTGCAACCGGTTCGCCAGCTACACTGGCGTCAACGCGCAACAATACCCGCCGCCAGGGCGCGGACCTTCGCTGATc                        >  1:49389/2‑251 (MQ=255)                                                                                                                                                                                                                    gCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGc                                                                                                                     <  1:393524/157‑1 (MQ=255)                                                                                                                                                                                                                    gCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATcc                       >  1:175462/1‑251 (MQ=255)                                                                                                                                                                                                                          ccAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGCTCGacan                                                                                                              <  1:275352/158‑2 (MQ=255)                                                                                                                                                                                                                          ccAGGAGCGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCNCCannn                                                                                                                                >  2:275352/1‑137 (MQ=255)                                                                                                                                                                                                                                gNGTTCCTGCATCCAGCGAGGATAGCCCTTAATTTTCCTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATgg                                                                                                                                                                             >  1:348152/1‑89 (MQ=255)                                                                                                                                                                                                                                    tCCTGCAGCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCGGCGCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTgn        <  1:110484/250‑2 (MQ=255)                                                                                                                                                                                                                                         cATCCAGCGAGGATAGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTat  >  1:7001/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             CGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCAGCACCTTATCAACCGGCACCGAATACACGATGCTTAGCGATGAGGGTGGCAGATTTTTCTTCAGCACCAGCTCCCGGAACCCTTCCGTATAGCCAAACCAGGAGCGTTCCTGCATCCAGCGAGGATCGCCCTTAATTTTACTTTCTGGTCCGGTAAGCGAAATCAGGGTATGACCATTTTCATCAAGAATGGTAACCCCCATCGGCAACGTACCCGGTAAGAAAAAGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTAT  >  NC_000913/2318832‑2319314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑