Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	38,145	G→A	T324I (ACC→ATC)	caiB ←	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	38,145	0	G	A	100.0%	53.0 / NA	21	T324I (ACC→ATC)	caiB	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/9);  total (12/9)

AGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCTGAGTGCCTTCCGGGATTTCTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGC  >  NC_000913/37900‑38385                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGTGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGGTGTTCGGCCCTTTGCAGGTGCGTTCATCCATCTTGTGCCACTGAGTGATTGATTCGCGAGTCATATACTGTGGATTGCTTTCCAGTTCCGGTACGATCacc                                                                                                                                                                                                                                             <  1:421004/251‑3 (MQ=255)                              aCCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGAGTCTAAGCCCTGTGAGGGCATTCCGCGACAGATTTGTCCGGGGTTATTTTTGACTTTCGGCATGATGTTCGGCCCTTTGCAGGCGCGACCATTCATCGTTTGCCACTGAGTGCTTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTCCGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGc                                                                                                                                                                                                                >  1:449355/1‑251 (MQ=255)                                     ccTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGat                                                                                                                                                                                                                            >  1:289274/1‑232 (MQ=255)                                                                                               atgTGAGTGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCTTGAGGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCATTTCCGGTACGAGCATCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTGTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCtt                                                                                                                                                     <  1:434182/245‑1 (MQ=255)                                                                                                      ggCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGATCATCGCTGTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCGGCTACTTCCGCGATGGTATGTGTCGCCATCCAGGCATCGAGTTTCTCTTCACCCAGTgggac                                                                                                                                        <  1:415099/251‑3 (MQ=255)                                                                                                                         gTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCCTCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGcgcgtaatgcgt                                                                                                                     >  1:337659/1‑240 (MQ=255)                                                                                                                                         gAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGTCCATCCATCGTTTGCCACGGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGATGAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGAGAAGCTGAGTGc                                                                                                     <  1:62648/251‑1 (MQ=255)                                                                                                                                                            ggCCCTTTGCAGGTGCGACCATCCATCTTGTGCCACTGAGTGATTGAGTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGGACGATCAGCACTTTGACGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTc                                                                                                                                                                                              <  1:293831/143‑1 (MQ=255)                                                                                                                                                                                 tccatcGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTGCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTGCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGGTTCTCTTCAACCATTGGTCCGTAAg                                                                                                                                   <  1:141262/181‑1 (MQ=255)                                                                                                                                                                                                         gattCGCGAGCCACATACTGTGGATTGCTTTACAGTTCCGGTACGATCAGCACTTTGGCGCAGAAGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATa                                                                                                               >  1:151167/1‑177 (MQ=255)                                                                                                                                                                                                         gattCGCGAGCAACATACTGTGGATTGCTTTCCAGTTACGGTACGATCCGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTACGCGATGGTATGTGTAGACAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCt                                                                                                           >  1:328623/1‑181 (MQ=255)                                                                                                                                                                                                              ncgAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGAGTTCTTTTACTTCAGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCTGAGTGCCTTCCggg                                                                                             >  1:42217/2‑190 (MQ=255)                                                                                                                                                                                                                 aGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGAAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCTGAGTGCCTTCCGGGATTTCTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTa                              >  1:305112/1‑250 (MQ=255)                                                                                                                                                                                                                    cacaTACTGTGGATTGCTTTCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCTGAGTGCCTTCCggg                                                                                             >  1:123241/1‑184 (MQ=255)                                                                                                                                                                                                                               gATTGCTTTCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCACAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAAc                                                                                                                                                 >  1:172791/1‑121 (MQ=255)                                                                                                                                                                                                                                aTTGCTTTCCAGTGCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAgg                                                                                                                                  <  1:283669/135‑1 (MQ=255)                                                                                                                                                                                                                                       tCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCANNCAGGCan                                                                                                                                                                 >  2:8047/1‑96 (MQ=255)                                                                                                                                                                                                                                       tCCAGTTCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAg                                                                                                                                   <  1:8047/127‑1 (MQ=255)                                                                                                                                                                                                                                           nttCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTAATTCCGCGATGGTATGTGTCGCCCGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCTGAGTGCCTTCCGGGATTTCTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGt     >  1:39686/2‑249 (MQ=255)                                                                                                                                                                                                                                             tCCGGTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGAGACGGTGGATAAGCTGAGTGCCTTCCGGGATTTCTTGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGc  <  1:307420/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gTACGATCAGCACTTTGGCGCAGACGATATTCAGTTCAGCAAAGCGTTATTTTACTTCcgcgctg                                                                                                                                                                                       >  1:209713/1‑62 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 AGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAATTTCGGCATGATGTTCGGCCCTTTGCAGGTGCGACCATCCATCGTTTGCCACTGAGTGATTGATTCGCGAGCCACATACTGTGGATTGCTTTCCAGTTCCGGTACGGTCAGCACTTTGGCGCAGGCGATATTCAGTTCAGCAAAGCGTTCTTTTACTTCCGCGATGGTATGTGTCGCCAGCCAGGCATCGAGTTTCTCTTCAACCAGTGGGCCGTAAGGGCATTCGATACGGTGGATAAGCTGAGTGCCTTCCGGGATTTCTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGC  >  NC_000913/37900‑38385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑