Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,208,132	0	G	A	56.0%	0.7 / 27.9	25	intergenic (‑600/‑15)	purH/rrsE	bifunctional AICAR transformylase/IMP cyclohydrolase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (6/5);  new base A (6/8);  total (12/13)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGC  >  NC_000913/4207897‑4208360                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                     cAGCCGGTTCGGTTGAAGAGAAAAATCCTGACATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATCAAACtttt                                                                                                                                                                                                                        >  1:120750/1‑250 (MQ=255)            gttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCTCCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGAaga                                                                                                                                                                                                              <  1:11056/249‑1 (MQ=255)                                          ttaCTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGCCAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggcgg                                                                                                                                                                              >  1:410728/3‑251 (MQ=25)                                                   aaGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTATTTAACAATTTATCAGACAATCTGCGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGCGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGAAGCGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTa                                                                                                                                                                      >  1:242764/1‑250 (MQ=255)                                                             cGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTGTAATTCTTTAAGCATCAAACTTTTAAATTGAAGAGGTTGATCATGGCTCAGATTTAACGCTGGCGGCAGGCCTAACACATGCAAg                                                                                                                                                           <  1:189364/251‑1 (MQ=255)                                                                       gCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAAGACCAAGTCTCAAGAGTTAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATc                                                                                                                                                                                                                                <  1:380041/172‑1 (MQ=255)                                                                                      tGCGCTAAAGCGCGTCGCAACTGCTCATTAACAATTTATCAGACAATCGGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTGAAGCATCAACCTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggc                                                                                                                                                                                <  1:307018/205‑1 (MQ=255)                                                                                          cTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCACACTATCTGTGAGGGCAGTCGAAGATACGCACTCTTAACATCGCAAGCAGACACATGAATACCAAGTCTCAAGAGTGAACCCGCAATTCATTACGACGTTTAATTCTTTAAGAATCaaa                                                                                                                                                                                                                             >  1:196550/1‑156 (MQ=2)                                                                                                                   aaCAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGATTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGCAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTg                                                                                                     >  1:2004/1‑251 (MQ=14)                                                                                                                   aaCAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAAAACCAAGTCTCAAGAGTGAACCCGTACTTCATTACGAAGTTTAATTCTTGGAGCATCAAACTTTTAAATTGAAGCGTTTGATCATGGCTCAGATTGAACGCTGGCGGCCGCCCTAACACATGCAAGTCGACCGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACggg                                                                                                       >  1:232692/1‑249 (MQ=11)                                                                                                                                                ntCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAg                                                                                                   >  1:114975/2‑224 (MQ=14)                                                                                                                                                                cTTAACGTCGCAAGACGAAAAATGAATACCACGTATCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTgg                                                         >  1:159722/1‑250 (MQ=14)                                                                                                                                                                cTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGTTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTg                                                                              <  1:141807/229‑1 (MQ=14)                                                                                                                                                                      gTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGt                                                  <  1:352552/251‑1 (MQ=31)                                                                                                                                                                        cGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAggggg                                                                    >  1:182297/1‑231 (MQ=31)                                                                                                                                                                        cGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGt                                                  >  1:392314/1‑249 (MQ=31)                                                                                                                                                                         gCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTAAGCAGCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTANc                                               <  1:347007/251‑1 (MQ=14)                                                                                                                                                                             gACGAAAAATGAATACCAAGTCTCATGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGCTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGGAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTaa                                            >  1:24057/1‑250 (MQ=14)                                                                                                                                                                               cGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTggn                                                                                                              <  1:43921/182‑2 (MQ=17)                                                                                                                                                                                gAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAggn                                                                      <  1:274770/221‑2 (MQ=14)                                                                                                                                                                                                    cAAGAGTGAACACTTAATTCATTACGAAGTTTAATTCTTTATGCAGCAAACTTTTAAATTGAAGAGTTTGATCATGGCGCAGATTGAACGCTGGCGGCAGGCCTGACACATGCTAGTCTAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTTGCGGAGTTGTGAGGAATTTCTGGGAAATTTCCTGAGGGTGGGGGATAACCATTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGAc                       <  1:326601/248‑1 (MQ=11)                                                                                                                                                                                                               acGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGTCCAAAGAGGGGGAcc         <  1:357062/251‑1 (MQ=14)                                                                                                                                                                                                                      tCATTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCggg   <  1:124270/250‑1 (MQ=14)                                                                                                                                                                                                                       cATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAAGTGAAGAGTTTGATCATGGCGCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCGGATGGAGGGGGATAACGACTGGAAACGGTAGCTAATACCTCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGc  <  1:204645/250‑1 (MQ=9)                                                                                                                                                                                                                        aTTACGAAGTTTAATTCTTTAAGCATCAAACTTTTAAATTGCAGAGTTTGATCATGGCTCAGATTGACCGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAggg              >  1:255325/1‑237 (MQ=9)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                     CAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGC  >  NC_000913/4207897‑4208360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑