Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,6760AG66.7% 7.8 / 21.0 34D70D (GAT→GAC) tfaQputative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base G (14/8);  total (21/13)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGC  >  NC_000913/1634441‑1634882
                                                                                                                                                                                                                                           |                                                                                                                                                                                                              
ccTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACCGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACATCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTCACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGAACTTCAACGAGATGc                                                                                                                                                                                                 >  1:167023/1‑251 (MQ=9)
ccTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCTTCCAGGATCCGGACCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGACGTTCCACTTCTGATCTTCTCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTAATAGACGGTTTTACCCCGATGGTCTTAAACGAGATGc                                                                                                                                                                                                 >  1:293074/1‑251 (MQ=11)
            naaTATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCAGGAACAGTTTTTCTGCTTCCGTATCATTCACCCAGGCTGTGCCGTTCCACTTCTGCTATTCCCCTCCCGACGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCtc                                                                                                                                                                                      >  1:40980/2‑250 (MQ=11)
                             aCCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTGTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGc                                                                                                                                                                    <  1:6768/251‑1 (MQ=11)
                                                        tcCGCCTCCCGGATCCGGAACAGTATTTCTGCTGCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTggn                                                                                                                                           <  1:114396/249‑2 (MQ=14)
                                                          cGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGGGCCGGTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGGCCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTg                                                                                                                                       <  1:28640/251‑1 (MQ=14)
                                                           nccTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCTTTCCCCCCGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGCAGCCACGTCATAAACCGTTTTACCCCGATGCTCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                                                                                                                                      >  1:82539/2‑251 (MQ=9)
                                                               cccGGATCCGGAACAGTTTTTATGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccagc                                                                                                                                                        >  1:102100/1‑229 (MQ=14)
                                                                      ccGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCCGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAc                                                                                                                           >  1:258774/1‑251 (MQ=11)
                                                                              gTTTTTCTGCTTCCGTATCCTTCACCNAGGCTGNNCCNTTCCACTTCTGATATTCCCNTCNNNGNNNTNCCCAGNTANNATTTTCCGGNAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACNGTTTTACCCNNATGGTCTTCAACGAGATGCCCNGATNNCNNANCACNGtn                                                                                                                                                                             >  2:95190/1‑192 (MQ=11)
                                                                                                   nacncctngnngnnCCGNTCCACTTCTGAAACTCCCCTCNNGTCGAGAACNAGGTACCATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGNAAGCCACGTNNTANACCGGNTNNNCNNNATNATCTTCAACGAGATGCCACNATNNCTCATCNCTGTTGAAAACAGCCACGAagccagc                                                                                                                                                        <  2:102100/185‑1 (MQ=11)
                                                                                                        cAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAACATTTTCCGGTCACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAcc                                                                                                           >  1:284034/1‑233 (MQ=11)
                                                                                                               tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGCGAACATTTTCTGGTCACGAACCGAGTTCAGAAATACATAAAGCGTCGCCGGAAGCCACGTCATCAACCGTTTTAACCCGATGATCTTCAACGAGATGCCACGCTGACTCATCACTGTTGAAAAAAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAATGTTTGCTGGCAGCCCTGTa                                                                                                       >  1:201672/1‑230 (MQ=11)
                                                                                                                   gTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACAAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCtt                                                                              >  1:308967/1‑251 (MQ=11)
                                                                                                                            ctgatattCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTGTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCAGCACTGTTGAAAACAGCCACAAAGCCAGTCGGAATATCTGGCGGTGCAATAGCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCan                                                                                 <  1:276879/232‑2 (MQ=11)
                                                                                                                              gatattCCCCTCCCGGCGATAACCAGGGAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCTGAAGCCACGTCATAGACGTTTTTCCCCCGATGGGCTGCAACGAGATGCCACGATGCCTCAGCACTGTTGAACACAGcc                                                                                                                                                                   <  1:338736/150‑1 (MQ=2)
                                                                                                                               atattCCCCTCCCGGCGATAACCAGGTAAAATGTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCAAAGACGGTTTTACCCCGATTGTCGTCAGCGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATg                                                                                                     <  1:237137/212‑1 (MQ=11)
                                                                                                                                aaCTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCCGACATAACTAACGCGTCGCCGGCAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCCCGATGACTCATCACTGTTGAACACAGCCACGCAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTa                                                                                                       >  1:227302/1‑213 (MQ=11)
                                                                                                                                            ngCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGaaa                                                                                                                                                                         >  1:131113/2‑135 (MQ=18)
                                                                                                                                            ggCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGCATATATGCGTCCCCTTCACCAATAAATTCATTAGTTCCGGc                                                     >  1:171716/1‑251 (MQ=11)
                                                                                                                                                                  ccGGTAACGGACCGAGTTCAGAAAGAAATAACGCGTCGCCGGAAGCCACGTCATATACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATg                               <  1:404807/251‑1 (MQ=11)
                                                                                                                                                                   nggTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGcc                                                                                                                                                                   >  1:87173/2‑118 (MQ=11)
                                                                                                                                                                     gTAACGGACCGAGTTCAGAAATAAATACCGCGGCGCCGGAAGCCACGCCATAAACCGTTTTCCCCCGATGATCTTCAACGAGATGCCACAATGACTCATCACTGTTGAAAACAGCCACGAagccagccn                                                                                                                                                      <  1:79453/129‑2 (MQ=11)
                                                                                                                                                                           gACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACACAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgt                      >  1:222565/1‑251 (MQ=11)
                                                                                                                                                                           gACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgt                      >  1:176322/1‑251 (MQ=11)
                                                                                                                                                                             ccGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgttg                    <  1:95190/251‑1 (MQ=11)
                                                                                                                                                                                 gTTCAGAAATAAATAACGCGTCGCCGNAAGCCANNTCNTAGACGGTTTTACCCCGATNGTNNNCNNNGNGATGCNACNNTGCCTCATCNCTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTNCTGTTTGccnnc                                                                                                                >  2:105074/1‑151 (MQ=11)
                                                                                                                                                                                 gTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGc                                                                                                                <  1:105074/155‑1 (MQ=11)
                                                                                                                                                                                  ttCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCANTATNNNNNCtnn                                                                                                                        >  2:270414/1‑144 (MQ=11)
                                                                                                                                                                                    naGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGACAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACAAATAAATTCATTAGTTCCGGCCAGCAGATTATaa                                        >  1:41931/2‑224 (MQ=11)
                                                                                                                                                                                        aataaatcACGCGTCGCCGGAAGCCACGTCATAAAACGTTTTACCCCGATGATCTTCACCGAGATGCCACGATGAATCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCg                                                                                                                               >  1:372417/1‑133 (MQ=11)
                                                                                                                                                                                               aaCGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGc  <  1:324205/251‑1 (MQ=31)
                                                                                                                                                                                                      cGCCGGAANCNNNGNNATAGACGGTTTTACCCCGATGGTCTTCTACGAGATGCCACGATGNCTCATCACTGTTGAANACNGCCANAAANCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc                                                                                                                    >  2:310301/1‑130 (MQ=11)
                                                                                                                                                                                                      cGCCGGAAGCCACGTCATAGACGGTTTGACCCCGATGGTCTTCTACGAGATGCCACGATGCCTCATCACTGGTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc                                                                                                                    <  1:310301/130‑1 (MQ=11)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                              
CCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGC  >  NC_000913/1634441‑1634882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑