Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,6580AG65.7% 9.1 / 20.2 36V76V (GTT→GTC) tfaQputative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base A (8/4);  new base G (15/8);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGC  >  NC_000913/1634419‑1634882
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                
gCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAAc                                                                                                                                                                                                                                >  1:2238/1‑242 (MQ=31)
                      ccTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACCGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACATCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTCACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGAACTTCAACGAGATGc                                                                                                                                                                                                 >  1:167023/1‑251 (MQ=9)
                      ccTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCTTCCAGGATCCGGACCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGACGTTCCACTTCTGATCTTCTCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTAATAGACGGTTTTACCCCGATGGTCTTAAACGAGATGc                                                                                                                                                                                                 >  1:293074/1‑251 (MQ=11)
                                 gcAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCACGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAAGCAGGTACAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCgat                                                                                                                                                                                                                  >  1:236763/1‑223 (MQ=11)
                                  naaTATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCAGGAACAGTTTTTCTGCTTCCGTATCATTCACCCAGGCTGTGCCGTTCCACTTCTGCTATTCCCCTCCCGACGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCtc                                                                                                                                                                                      >  1:40980/2‑250 (MQ=11)
                                                   aCCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTGTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGc                                                                                                                                                                    <  1:6768/251‑1 (MQ=11)
                                                                              tcCGCCTCCCGGATCCGGAACAGTATTTCTGCTGCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTggn                                                                                                                                           <  1:114396/249‑2 (MQ=14)
                                                                                cGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGGGCCGGTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGGCCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTg                                                                                                                                       <  1:28640/251‑1 (MQ=14)
                                                                                 nccTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCTTTCCCCCCGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGCAGCCACGTCATAAACCGTTTTACCCCGATGCTCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                                                                                                                                      >  1:82539/2‑251 (MQ=9)
                                                                                     cccGGATCCGGAACAGTTTTTATGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccagc                                                                                                                                                        >  1:102100/1‑229 (MQ=14)
                                                                                            ccGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCCGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAc                                                                                                                           >  1:258774/1‑251 (MQ=11)
                                                                                                    gTTTTTCTGCTTCCGTATCCTTCACCNAGGCTGNNCCNTTCCACTTCTGATATTCCCNTCNNNGNNNTNCCCAGNTANNATTTTCCGGNAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACNGTTTTACCCNNATGGTCTTCAACGAGATGCCCNGATNNCNNANCACNGtn                                                                                                                                                                             >  2:95190/1‑192 (MQ=11)
                                                                                                               tCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTAcc                                                                                                                                                                                                                       <  1:18343/140‑1 (MQ=11)
                                                                                                                         nacncctngnngnnCCGNTCCACTTCTGAAACTCCCCTCNNGTCGAGAACNAGGTACCATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGNAAGCCACGTNNTANACCGGNTNNNCNNNATNATCTTCAACGAGATGCCACNATNNCTCATCNCTGTTGAAAACAGCCACGAagccagc                                                                                                                                                        <  2:102100/185‑1 (MQ=11)
                                                                                                                              cAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAACATTTTCCGGTCACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGAcc                                                                                                           >  1:284034/1‑233 (MQ=11)
                                                                                                                                     tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGCGAACATTTTCTGGTCACGAACCGAGTTCAGAAATACATAAAGCGTCGCCGGAAGCCACGTCATCAACCGTTTTAACCCGATGATCTTCAACGAGATGCCACGCTGACTCATCACTGTTGAAAAAAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAATGTTTGCTGGCAGCCCTGTa                                                                                                       >  1:201672/1‑230 (MQ=11)
                                                                                                                                         gTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACAAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCtt                                                                              >  1:308967/1‑251 (MQ=11)
                                                                                                                                                  ctgatattCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTGTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCAGCACTGTTGAAAACAGCCACAAAGCCAGTCGGAATATCTGGCGGTGCAATAGCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCan                                                                                 <  1:276879/232‑2 (MQ=11)
                                                                                                                                                    gatattCCCCTCCCGGCGATAACCAGGGAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCTGAAGCCACGTCATAGACGTTTTTCCCCCGATGGGCTGCAACGAGATGCCACGATGCCTCAGCACTGTTGAACACAGcc                                                                                                                                                                   <  1:338736/150‑1 (MQ=2)
                                                                                                                                                     atattCCCCTCCCGGCGATAACCAGGTAAAATGTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCAAAGACGGTTTTACCCCGATTGTCGTCAGCGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATg                                                                                                     <  1:237137/212‑1 (MQ=11)
                                                                                                                                                      aaCTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCCGACATAACTAACGCGTCGCCGGCAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCCCGATGACTCATCACTGTTGAACACAGCCACGCAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTa                                                                                                       >  1:227302/1‑213 (MQ=11)
                                                                                                                                                                  ngCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGaaa                                                                                                                                                                         >  1:131113/2‑135 (MQ=18)
                                                                                                                                                                  ggCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGCATATATGCGTCCCCTTCACCAATAAATTCATTAGTTCCGGc                                                     >  1:171716/1‑251 (MQ=11)
                                                                                                                                                                                        ccGGTAACGGACCGAGTTCAGAAAGAAATAACGCGTCGCCGGAAGCCACGTCATATACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATg                               <  1:404807/251‑1 (MQ=11)
                                                                                                                                                                                         nggTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGcc                                                                                                                                                                   >  1:87173/2‑118 (MQ=11)
                                                                                                                                                                                           gTAACGGACCGAGTTCAGAAATAAATACCGCGGCGCCGGAAGCCACGCCATAAACCGTTTTCCCCCGATGATCTTCAACGAGATGCCACAATGACTCATCACTGTTGAAAACAGCCACGAagccagccn                                                                                                                                                      <  1:79453/129‑2 (MQ=11)
                                                                                                                                                                                                 gACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACACAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgt                      >  1:222565/1‑251 (MQ=11)
                                                                                                                                                                                                 gACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgt                      >  1:176322/1‑251 (MQ=11)
                                                                                                                                                                                                   ccGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGgttg                    <  1:95190/251‑1 (MQ=11)
                                                                                                                                                                                                       gTTCAGAAATAAATAACGCGTCGCCGNAAGCCANNTCNTAGACGGTTTTACCCCGATNGTNNNCNNNGNGATGCNACNNTGCCTCATCNCTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTNCTGTTTGccnnc                                                                                                                >  2:105074/1‑151 (MQ=11)
                                                                                                                                                                                                       gTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGc                                                                                                                <  1:105074/155‑1 (MQ=11)
                                                                                                                                                                                                        ttCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCANTATNNNNNCtnn                                                                                                                        >  2:270414/1‑144 (MQ=11)
                                                                                                                                                                                                          naGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGACAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACAAATAAATTCATTAGTTCCGGCCAGCAGATTATaa                                        >  1:41931/2‑224 (MQ=11)
                                                                                                                                                                                                              aataaatcACGCGTCGCCGGAAGCCACGTCATAAAACGTTTTACCCCGATGATCTTCACCGAGATGCCACGATGAATCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCg                                                                                                                               >  1:372417/1‑133 (MQ=11)
                                                                                                                                                                                                                     aaCGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGc  <  1:324205/251‑1 (MQ=31)
                                                                                                                                                                                                                            cGCCGGAANCNNNGNNATAGACGGTTTTACCCCGATGGTCTTCTACGAGATGCCACGATGNCTCATCACTGTTGAANACNGCCANAAANCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc                                                                                                                    >  2:310301/1‑130 (MQ=11)
                                                                                                                                                                                                                            cGCCGGAAGCCACGTCATAGACGGTTTGACCCCGATGGTCTTCTACGAGATGCCACGATGCCTCATCACTGGTGAAAACAGCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc                                                                                                                    <  1:310301/130‑1 (MQ=11)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                
GCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGC  >  NC_000913/1634419‑1634882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑