Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,0720AC53.8% ‑0.6 / 21.5 26V8V (GTA→GTC) stfRputative prophage side tail fiber protein StfR
Reads supporting (aligned to +/- strand):  ref base A (6/6);  new base C (9/5);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCACAGCAACACGTCTCTGGCGTGGGGAGCTGGCGTGCAGTTTAACCCGACCGAATCCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTC  >  NC_000913/1428840‑1429318
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
ncacaGCAACACGTCTCTGGCGTGGGGAGCTGGCGTGCAGTTTAACCCGACCGAATCCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAgg                                                                                                                                                                                                                                       >  1:112301/2‑250 (MQ=255)
                                                ggcagcgtccgtcgttgttGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAAcc                                                                                                                                                                                                                                 <  1:103446/191‑1 (MQ=255)
                                                ggcagcgtccgtcgttgttGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAAcc                                                                                                                                                                                                                                 <  1:103437/191‑1 (MQ=255)
                                                                                     cccGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTACCACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGGGTACTGAAAGACGGCACAg                                                                                                                                                                                                                                        >  1:60711/1‑164 (MQ=255)
                                                                                         gCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCAAGGTAACACAGTGTTATGACAGACCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCgg                                                                                                                                              >  1:106199/1‑250 (MQ=255)
                                                                                              ggCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGCTTAGCCAGGTACCACAGAGTTATGACAGCCAGCCGGTTCGGGCGGGCTTTTTTGTGGGGTGAATCTGGCAGTAAAGCTTTCAGGTGTCCTGAAAGACGGCCCAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGAGGTGAACACGCTGGCCACAGAAAAACCGGATGa                                                                                                                                          >  1:319870/1‑249 (MQ=255)
                                                                                               gCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGc                                                                                                                                       <  1:99226/251‑1 (MQ=255)
                                                                                                     gtcgtaCTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGCAAAACAGTGTTATGACAGAAAGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGAAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCgg                                                                                                                                                                                                                               >  1:407075/6‑157 (MQ=255)
                                                                                                             gACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACCCAGCGTTATGACAGCACGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCCGTAAAGATTTCAGGAGTCCTGACAGACgg                                                                                                                                                                                                                                             >  1:291517/1‑135 (MQ=255)
                                                                                                                 gTTTCAGCGTGGGTGTCGGTTATAAGTTCGGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTTAAAGACGTCACAGGAAAACCGTTTCAGAACTGCACAATCCAGCTGAAAGCAAAGCGTAACAGCACCTCGGTGGGGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGa                                                                                                                     <  1:217936/251‑1 (MQ=255)
                                                                                                                        cGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGGAACACAGTGTTATGCCAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATGTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTTCAGACCTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACGGCGGTGGTGGTGAACACGCGGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAg                                                                                                              <  1:138529/251‑1 (MQ=255)
                                                                                                                              ggtcgattagagtggctgtttAGGCAGGTAACACAGTGTTAGGACAGGCCGCCGGTTCAGGCGGGCGTTTTTGTGTGGTGAATATGGCAGTAAAGATTTCAGGTGTATTTGAAGACGGCACAGAAAAACCGGTACAGAACTTCACAAGCCAGCTGAAAGCAAAACGTAACAGCACCATGGTGGGGGGGAACACGCTGGCCTCAGAAAGTCCGGATGAAGCCGTGCGTTACAGCATGGACGTTGAGTACgg                                                                                                         <  1:72721/231‑1 (MQ=255)
                                                                                                                                        aaGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAAGATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACan                                                                                                                                                                                                                          <  1:91420/127‑2 (MQ=255)
                                                                                                                                             cTGATTAGCCAGGTAACACAGTGTTATGACAGCACGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatc                                                                                         >  1:353644/1‑244 (MQ=255)
                                                                                                                                                     ccAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAAcc                                                                                                                                                                                                                                 >  1:374717/1‑107 (MQ=255)
                                                                                                                                                         gTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCNAAANNNNNCann                                                                                                                                                                                      >  2:268111/1‑144 (MQ=255)
                                                                                                                                                         gTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGan                                                                                                              <  1:268111/218‑2 (MQ=255)
                                                                                                                                                         gTAACACAGTGTTATGACAGCCCGCCGGAACCGGCGGGCTTTTTTGTGGGGTGAATATGGGGGTAAAGATATCAGGCGTCCTGAAAGACGGCACAGGaaa                                                                                                                                                                                                                                    >  1:428418/1‑100 (MQ=255)
                                                                                                                                                               ccgtgtTATGTCAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAAACCAGCTGAAAGCAAAACGTAACATCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAATCCGAGCGTTACAGCAGGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGAttn                                                                       <  1:48785/249‑2 (MQ=255)
                                                                                                                                                                                gccggaaccggtgggCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGGCGGCACAGGAAAACCGTTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGGGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCa                                                                                                                         <  1:185238/172‑1 (MQ=255)
                                                                                                                                                                                         ggtgggCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACgg                                                                                                                                                                                                                                             >  2:36680/4‑59 (MQ=255)
                                                                                                                                                                                         ggtgggCTTTTTGGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACgg                                                                                                                                                                                                                                             <  1:36680/56‑1 (MQ=255)
                                                                                                                                                                                           cGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACCGGAAAAACGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGGTGGCCTCCGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCCGTACAGCGTTATTCTGTtggt                                                                                 >  1:235320/1‑213 (MQ=255)
                                                                                                                                                                                                           gTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATtctc                            >  1:24287/1‑250 (MQ=255)
                                                                                                                                                                                                                    gCAGTNACGATNTNNCGAGTCCTGATAGACGGCANAGGAAAACCGGTACANAACTNcaccantc                                                                                                                                                                                                             >  2:165689/1‑59 (MQ=255)
                                                                                                                                                                                                                                      gtACTGAAAGACGGCACAGGAAAACCGGTACAGCACTGCACAATCCAGATGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTtctc  >  1:66252/1‑249 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
CCACAGCAACACGTCTCTGGCGTGGGGAGCTGGCGTGCAGTTTAACCCGACCGAATCCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTC  >  NC_000913/1428840‑1429318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑