Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,0990A.65.6% 16.8 / 50.4 33intergenic (‑206/+85)borD/ybcVprophage lipoprotein BorD/DUF1398 domain‑containing protein YbcV
Reads supporting (aligned to +/- strand):  ref base A (6/5);  new base . (11/10);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-02
Rejected as consensus: Frequency below/above cutoff threshold.

GTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTG  >  NC_000913/578850‑579337
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
gTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTaaaaa                                                                                                                                                                                                                                                >  1:191627/1‑250 (MQ=255)
            gCCAGCGCAGTATCGAGTAGCATTTTTTTCATGGTGTTAGTCCCGATGCTTTGTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTTTATTCCCTGATTAACGATTTCCACAGCCCTGACGGGTAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAggg                                                                                                                                                                                                                                  <  1:395352/251‑1 (MQ=255)
            gCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAggg                                                                                                                                                                                                                                  >  1:29770/1‑251 (MQ=255)
                         ngAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAg                                                                                                                                                                                                                             >  1:358939/2‑243 (MQ=255)
                             tAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTAATAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATAAACTATGTCCACAGCCCTTACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAgg                                                                                                                                                                                                                                   <  1:220409/233‑1 (MQ=255)
                                         cATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGc                                                                                                                                                                                                      >  1:9627/1‑251 (MQ=255)
                                          aTGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATAGTGTTAATATTTAATAATGTATGTCAGGTGCGATTAATCGTCATTGGATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCTTACACGTATTGCATTATGCCa                                                                                                                                                                                                    <  1:442774/251‑1 (MQ=255)
                                                           gCTTTTTGAAGTACGCAGAATCGTAGGTTTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAAATTCATATTGTGAATATTTATTAAGGTATGTCAGGGGCGATGAATCGTCATTGTATTCCCGGATTAACTATTTCCACAGCCCTTTGGGGGAACTTCTCTTCGGGAGTGTCCTGTAAAAATTAAAA‑CGATGCAAGCAGGGTTAAGCGCGTGCGCGTATTGCATTATGCCAACGCCCCGGTGCTg                                                                                                                                                                                      <  1:395353/248‑1 (MQ=255)
                                                                         gCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATTTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCgg                                                                                                                                                                      >  1:405546/1‑250 (MQ=255)
                                                                                ngTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTAAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCt                                                                                                                                                                                       >  1:80389/2‑226 (MQ=255)
                                                                                          gAAAATTAAACAAACCCTAAACACTGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACg                                                                                                                                                                                 >  1:398070/1‑222 (MQ=255)
                                                                                           aaaaTTAAACAACCCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCGGACGGGGAACTTCTCTTCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCAGTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTgg                                                                                                                                                   <  1:62363/251‑1 (MQ=255)
                                                                                                            aaaCAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGGTATGATTTAGCGTGGAAAGATGTGTGTAgtg                                                                                                                                   <  1:342826/250‑1 (MQ=255)
                                                                                                                     gTTGAAATTTCATATTGTTAATCTTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAAt                                                                                                                           >  1:36478/1‑249 (MQ=255)
                                                                                                                        gAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctn                                                                                                                       <  1:87026/251‑2 (MQ=255)
                                                                                                                                    tGTTAATATTTATTAATGTATGTCAGNTGCGATNNATNGTCATTGTATTCCCGGATTNACNNNGNNNANCGCCCNGANNGGGAACTTCNCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAgcg                                                                                                                                                                                                                           >  2:102040/1‑138 (MQ=255)
                                                                                                                                    tGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAgcg                                                                                                                                                                                                                           <  1:102040/138‑1 (MQ=255)
                                                                                                                                                                 cGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGACCTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGgaag                                                                                                                                                                             >  1:78212/1‑155 (MQ=255)
                                                                                                                                                                         cGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTtg                                                                                                                                          >  1:355217/1‑182 (MQ=255)
                                                                                                                                                                             aTTGTATTCCAGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTATGCGGGAGTGTCAGGGAATAATTCAAACCGATGCAAAAAGGGGTTAGCGAGCACATGTATCGTATTATGCCAACACACCGGTGCTGACACGGCAGAAACCGGACGTTATGAtt                                                                                                                                                           >  1:406221/1‑162 (MQ=255)
                                                                                                                                                                                gTATTCCCGGATGAACTATTACCACAGCCCTGACGGGGAACTTCTCTGAGGGAGGGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTAGTATGCCAACACCCCGTTGCTGACACGGAAGAAACCGGACGTTATGATTTATCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGGAATGAATTATCAACGGTATAGTAATATCTTTTATGTTCGTgta                                                                <  1:19596/250‑3 (MQ=255)
                                                                                                                                                                                 tATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGct                                                                                                                        <  1:165207/192‑1 (MQ=255)
                                                                                                                                                                                     cccGGATTAACTATGTCCACAGCCCGGACGGGGAACTTCTCTGCGGGAGTGTCCTGGAATAAGTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAac                                                                                                                                                                                                  <  1:211113/115‑1 (MQ=255)
                                                                                                                                                                                        ggATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTACGGGAATACTTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACACCGGTGCTGACACGGAAGCAACCGGACGTTATGATTTAGCGTGGAAAGATTtg                                                                                                                                          >  1:388970/1‑168 (MQ=255)
                                                                                                                                                                                                       acaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTNCGGGAATAANNAANAACGANGNNNANNNGGNTTAGCGCGTACATGTATTGNATNNTGCCAANACCCCGGTGCTGACACGGAAGAAAcc                                                                                                                                                                        <  2:102527/123‑1 (MQ=255)
                                                                                                                                                                                                       acaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAAcc                                                                                                                                                                        >  1:102527/1‑123 (MQ=255)
                                                                                                                                                                                                          ncccTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATCATTAAAAACGATGCACCAAGGGTTTAGCCCGTACATGTATTGTATTATGCCAACACCCCGGCGCTGACACGGAAGAAACCGGACGTTCTGATTTAGCGTGGAACGTTTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTg                                                                  >  1:38657/2‑222 (MQ=38)
                                                                                                                                                                                                          gCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATACTTAAAA‑CGCTGCACACAGGGTTTAGCGCGTCCACGTATTGCATTATGCCAACGCCCCg                                                                                                                                                                                            >  1:295327/1‑99 (MQ=255)
                                                                                                                                                                                                          gCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATACACAAAGGGTTTAgc                                                                                                                                                                                                                            >  1:328776/1‑68 (MQ=255)
                                                                                                                                                                                                          gCCCTGACGGGGAACTTCTCTGCGGGAGNGTCCGGGAATAATTAAAAACGATNNACNNNGGNNTTAgc                                                                                                                                                                                                                            <  2:328776/68‑1 (MQ=255)
                                                                                                                                                                                                               gACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCAGTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCttt                                                                            <  1:385864/206‑1 (MQ=38)
                                                                                                                                                                                                                    ggAACTTATCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGATGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCa                             >  1:362897/1‑249 (MQ=255)
                                                                                                                                                                                                                               gCGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTGGCAGTAAATAGTAATGAATTATCGAATGTATAGTAATATCTTTGATGTGCATTGATATTTGTAGCCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTg                 <  1:328578/249‑1 (MQ=255)
                                                                                                                                                                                                                                cGNGAGTGTCCGGGAATAATTANAA‑NNATGCACNCAGGGTTTAGCGCGTACAcgtattgc                                                                                                                                                                                                             <  2:95453/60‑2 (MQ=255)
                                                                                                                                                                                                                                cGGGAGTGTCCGGGAATAATTAAAA‑CGATGCACACAGGGTTTAGCGCGTACAcgtattgc                                                                                                                                                                                                             >  1:95453/1‑59 (MQ=255)
                                                                                                                                                                                                                                                    ttaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAAc                                                                                                                                                                         <  1:28311/75‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAAc                                                                                                                                                                         >  2:28311/3‑77 (MQ=255)
                                                                                                                                                                                                                                                      nanaCNATGCANACAGNGTTTAGCGCGTACATNTATTGTATGATGCCAACACNNCNGTGCTNACACg                                                                                                                                                                                 <  2:191627/66‑1 (MQ=255)
                                                                                                                                                                                                                                                       aaaCGATGCACACAGGGTTTAACGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCATGGAAAGATTTGTGTAGTGTTCGGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTACGTTCATGGAGATTGGCAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAtgtg  <  1:434888/241‑1 (MQ=25)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
GTAATAAGCAGGGCCAGCGCAGTAGCGAGTAGCATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGAAAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTG  >  NC_000913/578850‑579337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑