| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,814,459 | Δ1 bp | coding (275/516 nt) | luxS ← | S‑ribosylhomocysteine lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,814,459 | 0 | A | . | 92.9% | 44.5 / ‑1.6 | 14 | coding (275/516 nt) | luxS | S‑ribosylhomocysteine lyase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (6/7); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGC > NC_000913/2814270‑2814686 | ntGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGata > 1:276790/2‑233 (MQ=255) gATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGc > 1:222835/1‑208 (MQ=255) naCGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCCTTGGCGAGATATCGATAATCTCTac > 1:275317/2‑231 (MQ=255) cGTTCCAGAAGGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCGTGTAAGTGCCACACTGGTAGATGTTCAGTTCCGGGATCTGATTCTGATCCTGCACGTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCGCAGCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGc < 1:210411/209‑1 (MQ=255) cAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGCAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGT‑CCCATTAGACTCATATAAAAACCGGTGCGGCAGCCAATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCAt > 1:223994/1‑251 (MQ=255) aCGCGCACTATCCTGCGCTTCCTGCAACGAGTGCATCTGGAAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGc > 1:156062/1‑251 (MQ=255) tCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGATATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAggg < 1:231092/249‑1 (MQ=255) naGTGCATCTGGTGAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACg > 1:110053/2‑211 (MQ=255) gTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑T‑AGACTCATATAAAAACAGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCActt > 1:301269/1‑251 (MQ=255) nnnnnnnnnngANNNNNAGTNCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGata < 2:276790/144‑1 (MQ=255) nnnnnnnnnnggNNNNNATTNTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTac < 2:275317/144‑1 (MQ=255) tCCATTTCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCTCAGATCGAACACGGTGATTGCGTCGCCATGCGGGgtg < 1:332832/248‑1 (MQ=255) aTTGTCGCTTGCCAGGCATCAGCGACACGCTGGTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAAtc < 1:213772/101‑1 (MQ=255) acacGCTGCGCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGgc < 1:404250/251‑1 (MQ=255) | CTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGC > NC_000913/2814270‑2814686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |