Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,550	Δ1 bp	coding (865/1206 nt)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,550	0	C	.	100.0%	52.8 / NA	14	coding (865/1206 nt)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (7/7);  total (7/7)

ATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTC  >  NC_000913/2360327‑2360772                                                                                                                                                                                                                                |                                                                                                                                                                                                                               aTCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAgtgtn                                                                                                                                                                                                    <  1:264643/251‑2 (MQ=255)                                                                                                                                   gtccccTGGAGTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGTTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGAGTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTGTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTGCAGTTTAGAGGGCGCGCAAGCGTgg                                                                   <  1:350695/248‑1 (MQ=255)                                                                                                                                      nccTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTcgc                                                                                                                                          >  1:129112/2‑176 (MQ=255)                                                                                                                                                ggCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGGGTGCCGTGGTGCGCACCGCTGGTTACCATCAGTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCAGACTGCTGGGGCGGCAGGCACTCGTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGtag                                                      <  1:428944/250‑3 (MQ=255)                                                                                                                                                          gCAATGTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTGTCAGTTCGCGATAACTTTCATACTGCTGTGGCggcaggc                                                                                                            <  1:68359/186‑1 (MQ=255)                                                                                                                                                              tttCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTGT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAGCTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAgg                                                                                 <  1:250921/209‑1 (MQ=255)                                                                                                                                                               ttCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTggc                                                                                                                   <  1:58758/174‑1 (MQ=255)                                                                                                                                                                     naGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCAt                                >  1:269874/2‑251 (MQ=255)                                                                                                                                                                                 accaccGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCCTCCTTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAg                                                                        >  1:176842/1‑199 (MQ=255)                                                                                                                                                                                              ncAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTc                                                                                                                                                 >  1:116399/2‑113 (MQ=255)                                                                                                                                                                                              ccAACATCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCggcaggca                                                                                                           >  1:341006/1‑151 (MQ=255)                                                                                                                                                                                                    tCCGGCTGCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGAGTGCCGTGGTGCTCCCCGCTGGTGACCATCCTTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAAACCTc  >  1:63205/1‑250 (MQ=255)                                                                                                                                                                                                           gCATAATGTCGATACCGGTTT‑TGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGGTTCATTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCAtt                                                                                             <  1:391363/152‑1 (MQ=255)                                                                                                                                                                                                                      aTACCGGATT‑TGACAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGAGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCca                             >  1:134354/1‑205 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                               ATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGTACGAAAAGATTGCAGTGTGCCGTGGTGCTCACCGCTGGTGACCATCATTCCGACTGGCGCGTTGCGTTTCAGTTCGCGATAACTTTCATACTGCTGTGGCGGCAGGCACTCTTCGATCCATTTCAGGTTATAGGGCGCGCAAGCGTGGGCCAGTTTGGTCGCATAGTTCACGTCCTGACTCATCCAGCAGTCGAGCATTAACCAGAAATCCTC  >  NC_000913/2360327‑2360772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑