Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,737,584	A→G	intergenic (‑39/‑260)	cydH ← / → purR	cytochrome bd‑I accessory subunit CydH/DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,737,584	0	A	G	100.0%	43.2 / NA	14	intergenic (‑39/‑260)	cydH/purR	cytochrome bd‑I accessory subunit CydH/DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/4);  total (10/4)

CGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACA‑GGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATATTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTG  >  NC_000913/1737339‑1737801                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          cGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACA‑GGACGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACg                                                                                                                                                                                                                      >  1:113482/1‑251 (MQ=255)       cATGACAGCAATCACCAAAAAATGAAAATAACAAAAACAGAAAACACTTTTGCAATTTTGCTA‑CAAAAACGGACGGCGATGCGCGGGAGCACGCGCTCCCTCGAGAGGAAATCCGTGCAGCGCGGCAGTCAAACCCACGGATACGATATAACCGAGGACGATACTCGTTGTAACCAGTGAAAATTTAAGGTCGGTGCTCATCACGTTTTCTCCTTTTTTATTACCACACAAACAGTGATGTTACGCAtttt                                                                                                                                                                                                                >  1:285651/1‑251 (MQ=255)        aTGACAGCAATCACAAAAAAATGAAAATAGCAAAAAGAGCACACACTTTTGCCATTTTGCTAATACACA‑GGAAGGAGCTGCGAGGGCGAACGCGCTCACTCGAGAGGAAATCAGTGCAGCGACGCAGACAAACCCACAGCTACGATCAAACCGAGGACGCTAATCGTTGATACAAGTGCAACTTTAAGGCCGGAGCTCATCAAGTTTTCTACTTTTTTATTACCACACAAAAAGTGATGTTACACAttttt                                                                                                                                                                                                               >  1:123992/1‑251 (MQ=255)                 aTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACA‑GGAAGGAGATGCGAGGGAGAACGCGCTCACTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACtgt                                                                                                                                                                                                      >  1:278279/1‑251 (MQ=255)                 aTCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTCTTGCCATTTTGCTAACAAACA‑GGAAGGAGATACGAGGGAGAACGCGCTCCCTCGAGAGGAAACAAGAGCAGCACGGCAGTCAAACCCACGGCTACGATCAAACCCAGGACGATAATCGTTGTTACCAGAGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACAACACAAAAAGTGATGTTACGCATTTTTACACACtgt                                                                                                                                                                                                      >  1:115370/1‑251 (MQ=255)                                       aaaaaGAGAAAACACTTTTGCCATTTTGCTAACAAACA‑GGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCACGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCAtt                                                                                                                                                                                >  1:161017/1‑251 (MQ=255)                                                                     aacaaaca‑GGAAGGAGCTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATACGTGTTTTTACCACTTcccc                                                                                                                                                  >  1:243570/1‑251 (MQ=255)                                                                                          naGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCa                                                                                                                               >  1:74160/2‑250 (MQ=255)                                                                                                                            gCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAAGCGCCCGTCATTGATAATGATAAGTGTTGTTACCACTTCCCCTTTACGTCAAGATCGGCCAAAATTc                                                                                                                         <  1:275573/222‑1 (MQ=255)                                                                                                                                         cAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTg                                                                               >  1:121775/1‑251 (MQ=255)                                                                                                                                                               ccGAGGACGATAATCGTTGTTACCATTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATTATAATTGTTTGTACGACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACTCTTACACGATTTGCGTACGGGCTATTGACCCCGTCCTGACGCTCCGTGTCGTTTTTCCgg                                                         <  1:188199/251‑1 (MQ=255)                                                                                                                                                                                        gTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTGTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGcn                                <  1:32601/251‑2 (MQ=255)                                                                                                                                                                                                           gCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATGTTACGCATTTTTACACACTGTGATGAAAAAAtctc                                                                                                                                                                                        >  1:153001/1‑80 (MQ=255)                                                                                                                                                                                                                       ttCTCCTTTTTTATTACCACACAAAAAGTGGTGTTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCAGTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTGCCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTg  <  1:254395/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                          CGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACA‑GGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGATATTACGCATTTTTACACACTGTGATGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTG  >  NC_000913/1737339‑1737801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑